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Literature summary extracted from
- Tomato gamma-glutamylhydrolases: expression, characterization, and evidence for heterodimer formation (2008), Plant Physiol., 148, 775-785.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.4.19.9 | when coexpressed in Escherichia coli, homodimer is catalytically inactive. LeGGH3 forms heterodimers with LeGGH1 or LeGGH2 that has one-half the activity of the matching homodimer | Solanum lycopersicum |
| 3.4.19.9 | when coexpressed in Escherichia coli, LeGGH1 forms heterodimers with an intermediate bond cleavage preference | Solanum lycopersicum |
| 3.4.19.9 | when coexpressed in Escherichia coli, LeGGH2 forms heterodimers with an intermediate bond cleavage preference | Solanum lycopersicum |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.9 | 0.00098 | - |
p-aminobenzoylpentaglutamate | - |
Solanum lycopersicum |  |
| 3.4.19.9 | 0.0012 | - |
folic acid pentaglutamate | - |
Solanum lycopersicum | |
| 3.4.19.9 | 0.00131 | - |
p-aminobenzoylpentaglutamate | - |
Solanum lycopersicum | |
| 3.4.19.9 | 0.00138 | - |
folic acid pentaglutamate | - |
Solanum lycopersicum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.4.19.9 | 36300 | - |
calculated from sequence | Solanum lycopersicum |
| 3.4.19.9 | 36400 | - |
calculated from sequence | Solanum lycopersicum |
| 3.4.19.9 | 36700 | - |
calculated from sequence | Solanum lycopersicum |
| 3.4.19.9 | 39000 | - |
SDS-PAGE | Solanum lycopersicum |
| 3.4.19.9 | 40000 | - |
SDS-PAGE | Solanum lycopersicum |
| 3.4.19.9 | 42000 | - |
SDS-PAGE | Solanum lycopersicum |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.9 | folic acid pentaglutamate + H2O | Solanum lycopersicum | PteGlu5 | ? | - |
? | |
| 3.4.19.9 | additional information | Solanum lycopersicum | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase | ? | - |
? | |
| 3.4.19.9 | additional information | Solanum lycopersicum | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than p-aminobenzoylpentaglutamate | ? | - |
? | |
| 3.4.19.9 | additional information | Solanum lycopersicum | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than pABAGlu5 | ? | - |
? | |
| 3.4.19.9 | p-aminobenzoylpentaglutamate + H2O | Solanum lycopersicum | pABAGlu5 | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | Solanum lycopersicum | B2Z9Y3 | Ailsa Craig | - |
| 3.4.19.9 | Solanum lycopersicum | B2Z9Y4 | Ailsa Craig | - |
| 3.4.19.9 | Solanum lycopersicum | B2Z9Y5 | Ailsa Craig | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.4.19.9 | recombinant proteins are purified by Ni2+ affinity chromatography | Solanum lycopersicum |
| 3.4.19.9 | recombinant proteins are purified by Ni2+ affinity chromatography; in the case of LeGGH1, an additional cation exchange step is required | Solanum lycopersicum |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.4.19.9 | flower bud | - |
Solanum lycopersicum | - |
| 3.4.19.9 | fruit | and all other organs | Solanum lycopersicum | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.4.19.9 | folic acid pentaglutamate + H2O | PteGlu5 | Solanum lycopersicum | ? | - |
? | |
| 3.4.19.9 | additional information | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase | Solanum lycopersicum | ? | - |
? | |
| 3.4.19.9 | additional information | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than p-aminobenzoylpentaglutamate | Solanum lycopersicum | ? | - |
? | |
| 3.4.19.9 | additional information | folates typically have gamma-linked polyglutamyl tails that make them better enzyme substrates and worse transport substrates than the unglutamylated forms. The tail can be shortened or removed by the vacuolar enzyme gamma-glutamyl hydrolase. Cleaves PteGlu5 more efficiently than pABAGlu5 | Solanum lycopersicum | ? | - |
? | |
| 3.4.19.9 | p-aminobenzoylpentaglutamate + H2O | pABAGlu5 | Solanum lycopersicum | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.4.19.9 | heterodimer | - |
Solanum lycopersicum |
| 3.4.19.9 | heterodimer | the homodimer is catalytically inactive | Solanum lycopersicum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.4.19.9 | gamma-glutamyl hydrolase | - |
Solanum lycopersicum |
| 3.4.19.9 | GGH | - |
Solanum lycopersicum |
| 3.4.19.9 | LeGGH1 | - |
Solanum lycopersicum |
| 3.4.19.9 | LeGGH2 | expressed in fruit all other organs | Solanum lycopersicum |
| 3.4.19.9 | LeGGH3 | expressed mainly in flower buds, the homodimer is catalytically inactive | Solanum lycopersicum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.4.19.9 | 0.96 | - |
p-aminobenzoylpentaglutamate | - |
Solanum lycopersicum | |
| 3.4.19.9 | 1.08 | - |
p-aminobenzoylpentaglutamate | - |
Solanum lycopersicum | |
| 3.4.19.9 | 1.69 | - |
folic acid pentaglutamate | - |
Solanum lycopersicum | |
| 3.4.19.9 | 2.13 | - |
folic acid pentaglutamate | - |
Solanum lycopersicum | |
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