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Literature summary extracted from

  • Wang, D.; Portis, A.R.; Moose, S.P.; Long, S.P.
    Cool C4 photosynthesis: pyruvate pi dikinase expression and activity corresponds to the exceptional cold tolerance of carbon assimilation in Miscanthus x giganteus (2008), Plant Physiol., 148, 557-567.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.9.1 additional information PPDK is light-activated, activation kinetics at different temperatures, overview Zea mays
2.7.9.1 additional information PPDK is light-activated, activation kinetics at different temperatures, overview Miscanthus x giganteus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.9.1 quantitative real-time reverse transcription (RT)-PCR expression analysis during transfer of plants from 25°C to 14°C growth temperature Zea mays
2.7.9.1 quantitative real-time reverse transcription (RT)-PCR expression analysis during transfer of plants from 25°C to 14°C growth temperature Miscanthus x giganteus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.9.1 additional information
-
 additional information kinetics at different temperature, the t1/2 for dephosphorylation of PPDK from chilling-acclimated Miscanthus x giganteus leaves increased to 2.3fold compared to warm-grown leaves, overview Miscanthus x giganteus
2.7.9.1 additional information
-
 additional information kinetics at different temperature, the t1/2 for dephosphorylation of PPDK from chilling-acclimated Miscanthus x giganteus leaves increased to 3.1fold compared to warm-grown leaves, overview Zea mays

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.9.1 chloroplast
-
 Zea mays 9507
-
2.7.9.1 chloroplast
-
 Miscanthus x giganteus 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 AMP + phosphoenolpyruvate + diphosphate Zea mays
-
 ATP + pyruvate + phosphate
-
 r
2.7.9.1 AMP + phosphoenolpyruvate + diphosphate Miscanthus x giganteus
-
 ATP + pyruvate + phosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.9.1 Miscanthus x giganteus
-
 a bioenergy feedstock grass
-
2.7.9.1 Zea mays P11155
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.9.1 leaf
-
 Zea mays
-
2.7.9.1 leaf
-
 Miscanthus x giganteus
-
2.7.9.1 additional information enzyme activity at different climates, cold climates at 14°C and warm climates at 25°C, overview Zea mays
-
2.7.9.1 additional information optimization of plant culture conditions, the unusual C4 species shows high productivity in cold climates, high activity at 14°C, while PPDK is the key limiting factor for C4 photosynthesis at low temperature in other plants, e.g. Zea mays, overview Miscanthus x giganteus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.9.1 AMP + phosphoenolpyruvate + diphosphate
-
 Zea mays ATP + pyruvate + phosphate
-
 r
2.7.9.1 AMP + phosphoenolpyruvate + diphosphate
-
 Miscanthus x giganteus ATP + pyruvate + phosphate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.9.1 PPDK
-
 Zea mays
2.7.9.1 PPDK
-
 Miscanthus x giganteus
2.7.9.1 pyruvate phosphate dikinase
-
 Zea mays
2.7.9.1 pyruvate phosphate dikinase
-
 Miscanthus x giganteus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.9.1 AMP forward reaction Zea mays
2.7.9.1 AMP forward reaction Miscanthus x giganteus
2.7.9.1 ATP reverse reaction Zea mays
2.7.9.1 ATP reverse reaction Miscanthus x giganteus