EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.3.25 | cDNA library screen, expression in Escherichia coli BL21(DE3), pCR4-TOPO, pET-28b vectors | Hordeum vulgare |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.25 | DL-myo-inositol 1-phosphate | - |
Hordeum vulgare | |
3.1.3.25 | Li+ | 0-4 mM LiCl, 3 mM Li+ inhibits activity by approximately 50%, inhibition increases with Li+ concentration | Hordeum vulgare |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.25 | 0.0097 | - |
DL-myo-inositol 1-phosphate | recombinant protein, 9.7 microM substrate, higher affinity as compared with reported Km values for other eukaryotic IMPases | Hordeum vulgare |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | 37000 | - |
SDS-PAGE | Hordeum vulgare |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.25 | Hordeum vulgare | B3DFH0 | cv. Harrington, wild-type and mutant | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.25 | gel filtration, recombinant protein | Hordeum vulgare |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.1.3.25 | leaf | 4 week old plants | Hordeum vulgare | - |
3.1.3.25 | root | 4 week old plants | Hordeum vulgare | - |
3.1.3.25 | seed | early development stage, wild-type and mutant plants | Hordeum vulgare | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.1.3.25 | additional information | - |
initial characterization of the genomic structure including promoter elements, expression of the single copy IMP-1 gene, expression levels not significantly altered by abiotic stress treatments, catalytic properties well suited for a role in myo-inositol synthesis | Hordeum vulgare |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.25 | D-myo-inositol 1-phosphate + H2O | - |
Hordeum vulgare | D-myo-inositol + phosphate | - |
? | |
3.1.3.25 | DL-myo-inositol 1-phosphate + H2O | - |
Hordeum vulgare | DL-myo-inositol + phosphate | - |
? | |
3.1.3.25 | L-myo-inositol 1-phosphate + H2O | - |
Hordeum vulgare | L-myo-inositol + phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.25 | ? | x * 37000, SDS-PAGE | Hordeum vulgare |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.25 | IMP-1 protein | - |
Hordeum vulgare |
3.1.3.25 | IMPase | - |
Hordeum vulgare |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.25 | 1.29 | - |
DL-myo-inositol 1-phosphate | recombinant protein | Hordeum vulgare |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.25 | 3.84 | - |
DL-myo-inositol 1-phosphate | recombinant protein | Hordeum vulgare |