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Literature summary extracted from

  • Chastain, C.J.; Xu, W.; Parsley, K.; Sarath, G.; Hibberd, J.M.; Chollet, R.
    The pyruvate, orthophosphate dikinase regulatory proteins of Arabidopsis possess a novel, unprecedented Ser/Thr protein kinase primary structure (2008), Plant J., 53, 854-863.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.27 His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana
2.7.11.32 His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3) Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.9.1 additional information phosphorylation of PPDK by AtRP1/2 is negated or greatly reduced when pyruvate is also included in the reaction mixture Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.4.27 chloroplast
-
 Arabidopsis thaliana 9507
-
2.7.9.1 chloroplast C3 chloroplasts Arabidopsis thaliana 9507
-
2.7.9.1 chloroplast C4 chloroplasts Zea mays 9507
-
2.7.11.32 chloroplast
-
 Arabidopsis thaliana 9507
-
2.7.11.32 cytosol
-
 Arabidopsis thaliana 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.9.1 additional information Zea mays differing functions of PPDK in C4 versus C3 plants, C4 PPDK regulatory proteins are involved in regulation of PPDK, mechanism, overview ?
-
 ?
2.7.9.1 additional information Arabidopsis thaliana differing functions of PPDK in C4 versus C3 plants, PPDK activity in C3 chloroplasts is light-regulated via reversible phosphorylation of an active-site Thr residue by the C3 PPDK regulatory proteins, most unusual bifunctional protein kinase /protein phosphatase, mechanism, overview. AtRP1 is chloroplast-targeted in predominant in greening and green tissues and organs, while AtRP2 is cytosol-localized mainly in seeds and pollen, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.27 Arabidopsis thaliana O49562
-
-
2.7.9.1 Arabidopsis thaliana
-
-
-
2.7.9.1 Zea mays
-
-
-
2.7.11.32 Arabidopsis thaliana O49562
-
-
2.7.11.32 Arabidopsis thaliana Q9MAC9
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.9.1 phosphoprotein PPDK activity in C3 chloroplasts is light-regulated via reversible phosphorylation of an active-site Thr residue by the PPDK regulatory protein, i.e. C3 RP, a most unusual bifunctional protein kinase /protein phosphatase, overview Arabidopsis thaliana
2.7.9.1 phosphoprotein PPDK activity in C4 chloroplasts is light-regulated via reversible phosphorylation of an active-site Thr residue by the PPDK regulatory protein, i.e. C4 RP, a most unusual bifunctional protein kinase /protein phosphatase, overview Zea mays

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.27 immobilized metal ion affinity chromatography (Ni2+) Arabidopsis thaliana
2.7.11.32 immobilized metal ion affinity chromatography (Ni2+) Arabidopsis thaliana

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.4.27 additional information green tissues Arabidopsis thaliana
-
2.7.11.32 additional information green tissues Arabidopsis thaliana
-
2.7.11.32 pollen
-
 Arabidopsis thaliana
-
2.7.11.32 seed
-
 Arabidopsis thaliana
-
2.7.11.32 stamen
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.27 [pyruvate, phosphate dikinase] phosphate + phosphate His458 and Thr456 phosphorylated (inactive), strict requirement for the threonyl phosphate, unable to dephosphorylate Ser-P at this same position Arabidopsis thaliana pyruvate, phosphate dikinase + diphosphate His458 phosphorylated (active) ?
2.7.9.1 additional information differing functions of PPDK in C4 versus C3 plants, C4 PPDK regulatory proteins are involved in regulation of PPDK, mechanism, overview Zea mays ?
-
 ?
2.7.9.1 additional information differing functions of PPDK in C4 versus C3 plants, PPDK activity in C3 chloroplasts is light-regulated via reversible phosphorylation of an active-site Thr residue by the C3 PPDK regulatory proteins, most unusual bifunctional protein kinase /protein phosphatase, mechanism, overview. AtRP1 is chloroplast-targeted in predominant in greening and green tissues and organs, while AtRP2 is cytosol-localized mainly in seeds and pollen, overview Arabidopsis thaliana ?
-
 ?
2.7.11.32 pyruvate, phosphate dikinase + ADP His458 phosphorylated (active), maize wild type and T456S mutant pyruvate, phosphate dikinase serve as substrate, T456V mutant pyruvate, phosphate dikinase serves not as substrate Arabidopsis thaliana [pyruvate, phosphate dikinase] phosphate + AMP His458 and Thr456 phosphorylated (inactive) ?
2.7.11.32 pyruvate, phosphate dikinase + ADP His458 phosphorylated, active Arabidopsis thaliana [pyruvate, phosphate dikinase] phosphate + AMP His458 and Thr456 phosphorylated (inactive) ?

Synonyms

EC Number Synonyms Comment Organism
2.7.4.27 AtRP1
-
 Arabidopsis thaliana
2.7.4.27 pyruvate, orthophosphate dikinase regulatory protein 1 bifunctional protein kinase/protein phosphatase Arabidopsis thaliana
2.7.9.1 PPDK
-
 Zea mays
2.7.9.1 PPDK
-
 Arabidopsis thaliana
2.7.9.1 pyruvate, orthophosphate dikinase
-
 Zea mays
2.7.9.1 pyruvate, orthophosphate dikinase
-
 Arabidopsis thaliana
2.7.11.32 AtRP1
-
 Arabidopsis thaliana
2.7.11.32 AtRP2
-
 Arabidopsis thaliana
2.7.11.32 pyruvate, orthophosphate dikinase regulatory protein 1 bifunctional Ser/Thr protein kinase/protein phosphatase Arabidopsis thaliana
2.7.11.32 pyruvate, orthophosphate dikinase regulatory protein 2 monofunctional Ser/Thr protein kinase Arabidopsis thaliana