BRENDA - Enzyme Database show

Protein plasticity: a single amino acid substitution in the Saccharomyces cerevisiae oxidosqualene-lanosterol cyclase generates protosta-13(17),24-dien-3beta-ol, a rearrangement product

Wu, T.K.; Wen, H.Y.; Chang, C.H.; Liu, Y.T.; Org. Lett. 10, 2529-2532 (2008)

Data extracted from this reference:

Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.7
F699C
inactive
Saccharomyces cerevisiae
5.4.99.7
F699Q
inactive
Saccharomyces cerevisiae
5.4.99.7
F699S
inactive
Saccharomyces cerevisiae
5.4.99.7
F699T
the mutant produces protosta-13(17),24-dien-3beta-ol from (S)-2,3-oxidosqualene
Saccharomyces cerevisiae
5.4.99.7
F699Y
inactive
Saccharomyces cerevisiae
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.4.99.7
Saccharomyces cerevisiae
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.7
(S)-2,3-oxidosqualene
-
694523
Saccharomyces cerevisiae
lanosterol
-
-
-
?
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.4.99.7
F699C
inactive
Saccharomyces cerevisiae
5.4.99.7
F699Q
inactive
Saccharomyces cerevisiae
5.4.99.7
F699S
inactive
Saccharomyces cerevisiae
5.4.99.7
F699T
the mutant produces protosta-13(17),24-dien-3beta-ol from (S)-2,3-oxidosqualene
Saccharomyces cerevisiae
5.4.99.7
F699Y
inactive
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.4.99.7
(S)-2,3-oxidosqualene
-
694523
Saccharomyces cerevisiae
lanosterol
-
-
-
?