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Literature summary extracted from
- Structural and evolutionary classification of type II restriction enzymes based on theoretical and experimental analyses (2008), Nucleic Acids Res., 36, 3552-3569.
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.21.4 | Mg2+ | required for activity | Thermus aquaticus |  |
| 3.1.21.4 | Mg2+ | required for activity | Sulfolobus acidocaldarius | |
| 3.1.21.4 | Mg2+ | required for activity | Helicobacter pylori | |
| 3.1.21.4 | Mg2+ | required for activity | Methylococcus capsulatus | |
| 3.1.21.4 | Mg2+ | required for activity | Trichormus variabilis | |
| 3.1.21.4 | Mg2+ | required for activity | Pyrococcus horikoshii | |
| 3.1.21.4 | Mg2+ | required for activity | Caryophanon latum | |
| 3.1.21.4 | Mg2+ | required for activity | Staphylococcus hominis | |
| 3.1.21.4 | Mg2+ | required for activity | Corynebacterium striatum | |
| 3.1.21.4 | Mg2+ | required for activity | Herpetosiphon aurantiacus | |
| 3.1.21.4 | Mg2+ | required for activity | Xanthomonas vasicola | |
| 3.1.21.4 | Mg2+ | required for activity | Staphylococcus aureus | |
| 3.1.21.4 | Mg2+ | required for activity | Nostoc sp. | |
| 3.1.21.4 | Mg2+ | required for activity | Micrococcus sp. | |
| 3.1.21.4 | Mg2+ | required for activity | Sphaerotilus natans | |
| 3.1.21.4 | Mg2+ | required for activity | Methanocaldococcus jannaschii | |
| 3.1.21.4 | Mg2+ | required for activity | Haemophilus parahaemolyticus | |
| 3.1.21.4 | Mg2+ | required for activity | Thermus filiformis | |
| 3.1.21.4 | Mg2+ | required for activity | Sporosarcina sp. | |
| 3.1.21.4 | Mg2+ | required for activity | Geobacillus stearothermophilus | |
| 3.1.21.4 | Mg2+ | required for activity | Neisseria meningitidis | |
| 3.1.21.4 | Mg2+ | required for activity | Cereibacter sphaeroides | |
| 3.1.21.4 | Mg2+ | required for activity | Serratia marcescens | |
| 3.1.21.4 | Mg2+ | required for activity | Lactococcus lactis | |
| 3.1.21.4 | Mg2+ | required for activity | Thalassobius gelatinovorus | |
| 3.1.21.4 | Mg2+ | required for activity | Streptomyces achromogenes | |
| 3.1.21.4 | Mg2+ | required for activity | Moraxella sp. | |
| 3.1.21.4 | Mg2+ | required for activity | Citrobacter freundii | |
| 3.1.21.4 | Mg2+ | required for activity | Haemophilus influenzae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.4 | Caryophanon latum | - |
- |
- |
| 3.1.21.4 | Cereibacter sphaeroides | Q6SA27 | - |
- |
| 3.1.21.4 | Citrobacter freundii | Q04852 | - |
- |
| 3.1.21.4 | Corynebacterium striatum | - |
- |
- |
| 3.1.21.4 | Corynebacterium striatum M82B | - |
- |
- |
| 3.1.21.4 | Geobacillus stearothermophilus | Q8L3A5 | - |
- |
| 3.1.21.4 | Haemophilus influenzae | P20588 | - |
- |
| 3.1.21.4 | Haemophilus parahaemolyticus | P00643 | - |
- |
| 3.1.21.4 | Helicobacter pylori | - |
- |
- |
| 3.1.21.4 | Helicobacter pylori | Q9ZLF0 | - |
- |
| 3.1.21.4 | Helicobacter pylori | Q9ZN14 | - |
- |
| 3.1.21.4 | Herpetosiphon aurantiacus | P25260 | - |
- |
| 3.1.21.4 | Lactococcus lactis | Q93K18 | subspecies Lactococcus lactis cremoris | - |
| 3.1.21.4 | Methanocaldococcus jannaschii | Q58723 | - |
- |
| 3.1.21.4 | Methanocaldococcus jannaschii | Q58895 | - |
- |
| 3.1.21.4 | Methylococcus capsulatus | - |
- |
- |
| 3.1.21.4 | Micrococcus sp. | Q2I0D9 | - |
- |
| 3.1.21.4 | Micrococcus sp. NEB 446 | Q2I0D9 | - |
- |
| 3.1.21.4 | Moraxella sp. | Q6S4U8 | - |
- |
| 3.1.21.4 | Moraxella sp. A1 | Q6S4U8 | - |
- |
| 3.1.21.4 | Neisseria meningitidis | Q9RLM3 | serogroup C | - |
| 3.1.21.4 | Nostoc sp. | P35677 | strain PCC 7524 | - |
| 3.1.21.4 | Pyrococcus horikoshii | - |
- |
- |
| 3.1.21.4 | Serratia marcescens | P14229 | - |
- |
| 3.1.21.4 | Sphaerotilus natans | Q6SA25 | - |
- |
| 3.1.21.4 | Sporosarcina sp. | Q194N8 | - |
- |
| 3.1.21.4 | Staphylococcus aureus | P23736 | - |
- |
| 3.1.21.4 | Staphylococcus hominis | - |
- |
- |
| 3.1.21.4 | Streptomyces achromogenes | O31074 | - |
- |
| 3.1.21.4 | Sulfolobus acidocaldarius | - |
- |
- |
| 3.1.21.4 | Thalassobius gelatinovorus | Q9KHV6 | - |
- |
| 3.1.21.4 | Thermus aquaticus | - |
- |
- |
| 3.1.21.4 | Thermus filiformis | Q9F8S3 | - |
- |
| 3.1.21.4 | Trichormus variabilis | - |
strain ATCC 27892 | - |
| 3.1.21.4 | Xanthomonas vasicola | Q9KVZ7 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.4 | DNA + H2O | - |
Thermus aquaticus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Sulfolobus acidocaldarius | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Helicobacter pylori | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Methylococcus capsulatus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Trichormus variabilis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Pyrococcus horikoshii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Caryophanon latum | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Staphylococcus hominis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Corynebacterium striatum | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Herpetosiphon aurantiacus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Xanthomonas vasicola | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Staphylococcus aureus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Nostoc sp. | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Micrococcus sp. | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Sphaerotilus natans | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Methanocaldococcus jannaschii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Haemophilus parahaemolyticus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Thermus filiformis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Sporosarcina sp. | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Geobacillus stearothermophilus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Neisseria meningitidis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Cereibacter sphaeroides | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Serratia marcescens | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Lactococcus lactis | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Thalassobius gelatinovorus | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Streptomyces achromogenes | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Moraxella sp. | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Citrobacter freundii | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Haemophilus influenzae | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Corynebacterium striatum M82B | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Moraxella sp. A1 | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
| 3.1.21.4 | DNA + H2O | - |
Micrococcus sp. NEB 446 | double-stranded DNA fragments with terminal 5'-phosphates | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.4 | AgeI | - |
Thalassobius gelatinovorus |
| 3.1.21.4 | AvaII | - |
Trichormus variabilis |
| 3.1.21.4 | BssSI | - |
Geobacillus stearothermophilus |
| 3.1.21.4 | CfrBI | - |
Citrobacter freundii |
| 3.1.21.4 | CstMI | - |
Corynebacterium striatum |
| 3.1.21.4 | CviAI | - |
Caryophanon latum |
| 3.1.21.4 | HgiEI | - |
Herpetosiphon aurantiacus |
| 3.1.21.4 | HhaII | - |
Haemophilus parahaemolyticus |
| 3.1.21.4 | HinfI | - |
Haemophilus influenzae |
| 3.1.21.4 | Hpy8I | - |
- |
| 3.1.21.4 | Hpy99II | - |
Helicobacter pylori |
| 3.1.21.4 | Hpy99IV | - |
Helicobacter pylori |
| 3.1.21.4 | Hpy99VIIIP | - |
Helicobacter pylori |
| 3.1.21.4 | LlaDI | - |
Lactococcus lactis |
| 3.1.21.4 | McaTI | - |
Methylococcus capsulatus |
| 3.1.21.4 | MjaIV | - |
Methanocaldococcus jannaschii |
| 3.1.21.4 | MjaV | - |
Methanocaldococcus jannaschii |
| 3.1.21.4 | MseI | - |
Micrococcus sp. |
| 3.1.21.4 | MspA1I | - |
Moraxella sp. |
| 3.1.21.4 | NmeDIP | - |
Neisseria meningitidis |
| 3.1.21.4 | NspV | - |
Nostoc sp. |
| 3.1.21.4 | PhoI | - |
Pyrococcus horikoshii |
| 3.1.21.4 | REase | - |
Thermus aquaticus |
| 3.1.21.4 | REase | - |
Sulfolobus acidocaldarius |
| 3.1.21.4 | REase | - |
Helicobacter pylori |
| 3.1.21.4 | REase | - |
Methylococcus capsulatus |
| 3.1.21.4 | REase | - |
Trichormus variabilis |
| 3.1.21.4 | REase | - |
Pyrococcus horikoshii |
| 3.1.21.4 | REase | - |
Caryophanon latum |
| 3.1.21.4 | REase | - |
Staphylococcus hominis |
| 3.1.21.4 | REase | - |
Corynebacterium striatum |
| 3.1.21.4 | REase | - |
Herpetosiphon aurantiacus |
| 3.1.21.4 | REase | - |
- |
| 3.1.21.4 | REase | - |
Xanthomonas vasicola |
| 3.1.21.4 | REase | - |
Staphylococcus aureus |
| 3.1.21.4 | REase | - |
Nostoc sp. |
| 3.1.21.4 | REase | - |
Micrococcus sp. |
| 3.1.21.4 | REase | - |
Sphaerotilus natans |
| 3.1.21.4 | REase | - |
Methanocaldococcus jannaschii |
| 3.1.21.4 | REase | - |
Haemophilus parahaemolyticus |
| 3.1.21.4 | REase | - |
Thermus filiformis |
| 3.1.21.4 | REase | - |
Sporosarcina sp. |
| 3.1.21.4 | REase | - |
Geobacillus stearothermophilus |
| 3.1.21.4 | REase | - |
Neisseria meningitidis |
| 3.1.21.4 | REase | - |
Cereibacter sphaeroides |
| 3.1.21.4 | REase | - |
Serratia marcescens |
| 3.1.21.4 | REase | - |
Lactococcus lactis |
| 3.1.21.4 | REase | - |
Thalassobius gelatinovorus |
| 3.1.21.4 | REase | - |
Streptomyces achromogenes |
| 3.1.21.4 | REase | - |
Moraxella sp. |
| 3.1.21.4 | REase | - |
Citrobacter freundii |
| 3.1.21.4 | REase | - |
Haemophilus influenzae |
| 3.1.21.4 | RsaI | - |
Cereibacter sphaeroides |
| 3.1.21.4 | SacI | - |
Streptomyces achromogenes |
| 3.1.21.4 | sau96I | - |
Staphylococcus aureus |
| 3.1.21.4 | Sho27844P | - |
Staphylococcus hominis |
| 3.1.21.4 | SmaI | - |
Serratia marcescens |
| 3.1.21.4 | SnaBI | - |
Sphaerotilus natans |
| 3.1.21.4 | Sse9I | - |
Sporosarcina sp. |
| 3.1.21.4 | SuaI | - |
Sulfolobus acidocaldarius |
| 3.1.21.4 | TaqII | - |
Thermus aquaticus |
| 3.1.21.4 | TfiI | - |
Thermus filiformis |
| 3.1.21.4 | type II restriction enzyme | - |
Thermus aquaticus |
| 3.1.21.4 | type II restriction enzyme | - |
Sulfolobus acidocaldarius |
| 3.1.21.4 | type II restriction enzyme | - |
Helicobacter pylori |
| 3.1.21.4 | type II restriction enzyme | - |
Methylococcus capsulatus |
| 3.1.21.4 | type II restriction enzyme | - |
Trichormus variabilis |
| 3.1.21.4 | type II restriction enzyme | - |
Pyrococcus horikoshii |
| 3.1.21.4 | type II restriction enzyme | - |
Caryophanon latum |
| 3.1.21.4 | type II restriction enzyme | - |
Staphylococcus hominis |
| 3.1.21.4 | type II restriction enzyme | - |
Corynebacterium striatum |
| 3.1.21.4 | type II restriction enzyme | - |
Herpetosiphon aurantiacus |
| 3.1.21.4 | type II restriction enzyme | - |
- |
| 3.1.21.4 | type II restriction enzyme | - |
Xanthomonas vasicola |
| 3.1.21.4 | type II restriction enzyme | - |
Staphylococcus aureus |
| 3.1.21.4 | type II restriction enzyme | - |
Nostoc sp. |
| 3.1.21.4 | type II restriction enzyme | - |
Micrococcus sp. |
| 3.1.21.4 | type II restriction enzyme | - |
Sphaerotilus natans |
| 3.1.21.4 | type II restriction enzyme | - |
Methanocaldococcus jannaschii |
| 3.1.21.4 | type II restriction enzyme | - |
Haemophilus parahaemolyticus |
| 3.1.21.4 | type II restriction enzyme | - |
Thermus filiformis |
| 3.1.21.4 | type II restriction enzyme | - |
Sporosarcina sp. |
| 3.1.21.4 | type II restriction enzyme | - |
Geobacillus stearothermophilus |
| 3.1.21.4 | type II restriction enzyme | - |
Neisseria meningitidis |
| 3.1.21.4 | type II restriction enzyme | - |
Cereibacter sphaeroides |
| 3.1.21.4 | type II restriction enzyme | - |
Serratia marcescens |
| 3.1.21.4 | type II restriction enzyme | - |
Lactococcus lactis |
| 3.1.21.4 | type II restriction enzyme | - |
Thalassobius gelatinovorus |
| 3.1.21.4 | type II restriction enzyme | - |
Streptomyces achromogenes |
| 3.1.21.4 | type II restriction enzyme | - |
Moraxella sp. |
| 3.1.21.4 | type II restriction enzyme | - |
Citrobacter freundii |
| 3.1.21.4 | type II restriction enzyme | - |
Haemophilus influenzae |
| 3.1.21.4 | XhoI | - |
Xanthomonas vasicola |
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