Any feedback?
Literature summary extracted from
- Characterization of the full length uracil-DNA glycosylase in the extreme thermophile Thermotoga maritima (2001), Mutat. Res., 485, 187-195.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.2.27 | gene tmudg, expression of His-tagged TMUDG in Escherichia coli strain BW310(DE3), TMUDG can substitute for the endogenous enzyme in Escherichia coli | Thermotoga maritima |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.2.27 | additional information | weak product inhibition of TMUDG by uracil | Thermotoga maritima |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.27 | 20578 | - |
x * 20578, sequence calculation | Thermotoga maritima |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.2.27 | Thermotoga maritima | - |
gene tmudg | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.2.27 | recombinant His-tagged TMUDG from Escherichia coli strain BW310(DE3) by nickel affinity and ion exchange chromatography, tag cleavage by thrombin | Thermotoga maritima |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.2.27 | additional information | optimal growth temperature of the organism is 80-90°C | Thermotoga maritima | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.2.27 | additional information | the enzyme initiates base excision repair, BER, using a closed circular DNA substrate containing a unique U-G base pair | Thermotoga maritima | ? | - |
? |  |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.2.27 | ? | x * 20578, sequence calculation | Thermotoga maritima |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.2.27 | TMUDG | - |
Thermotoga maritima |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.27 | 75 | - |
the enzyme has an optimal activity temperature below the growth temperature of the organism of 80-90°C | Thermotoga maritima |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.27 | 70 | 85 | - |
Thermotoga maritima |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.27 | additional information | - |
heat inactivation kinetics of the truncated and full length TMUDG, overview | Thermotoga maritima |
| 3.2.2.27 | 70 | 75 | highly stable | Thermotoga maritima |
| 3.2.2.27 | 75 | - |
purified recombinant enzyme, 2.5 h, completely stable | Thermotoga maritima |
| 3.2.2.27 | 85 | - |
purified recombinant enzyme, after 15 min completely stable, after 30 min loss of 80% of activity | Thermotoga maritima |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.2.27 | 7.5 | - |
assay at | Thermotoga maritima |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
