EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.2.27 | expression in Escherichia coli | Methanocaldococcus jannaschii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.2.2.27 | D150E | site-directed mutagenesis, inactive mutant | Methanocaldococcus jannaschii |
3.2.2.27 | D150E | displays reduced activity of about 70% of the wild type value | Methanocaldococcus jannaschii |
3.2.2.27 | D150W | site-directed mutagenesis, the mutant shows 70% of wild-type enzyme activity | Methanocaldococcus jannaschii |
3.2.2.27 | D150W | completely lacks DNA glycosylase activity | Methanocaldococcus jannaschii |
3.2.2.27 | E132K | site-directed mutagenesis, a mutation in the HhH motif with a lysine residue equivalent to Lys120 in endonuclease III leading to conversion of the enzyme into a bifunctional glycosylase/AP lyase capable of both removing uracil at a glycosylic bond and cleaving the phosphodiester backbone at an AP site. Mutant E132K catalyzes a beta-elimination reaction at the AP site via uracil excision and forms a Schiff base intermediate in the form of a protein-DNA complex | Methanocaldococcus jannaschii |
3.2.2.27 | E132K | mutation converts the enzyme into a bifunctional glycosylase/AP lyase capable of both removing uracil at a glycosylic bond and cleaving the phosphodiester backbone at an apurinic/apyrimidinic site. The mutant catalyzes a beta-elimination reaction at the apurinic/apyrimidinic site via uracil excision and forms a Schiff base intermediate in the form of a protein-DNA complex | Methanocaldococcus jannaschii |
3.2.2.27 | Y152E | site-directed mutagenesis, the mutant shows unaltered enzyme activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
3.2.2.27 | Y152E | retains unchanged levels of uracil-DNA glycosylase activity | Methanocaldococcus jannaschii |
3.2.2.27 | Y152N | site-directed mutagenesis, the mutant shows unaltered enzyme activity compared to the wild-type enzyme | Methanocaldococcus jannaschii |
3.2.2.27 | Y152N | retains unchanged levels of uracil-DNA glycosylase activity | Methanocaldococcus jannaschii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 24000 | - |
x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.2.27 | Methanocaldococcus jannaschii | - |
- |
- |
3.2.2.27 | Methanocaldococcus jannaschii | Q58829 | - |
- |
3.2.2.27 | Methanocaldococcus jannaschii DSM 2661 | Q58829 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.2.27 | - |
Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.2.27 | uracil-mismatched double-stranded oligonucleotide + H2O | oligonucleotide duplex containing a U:T mispair | Methanocaldococcus jannaschii | uracil + double-stranded oligonucleotide with abasic site | - |
? | |
3.2.2.27 | uracil-mismatched double-stranded oligonucleotide + H2O | oligonucleotide duplex containing a U:T mispair | Methanocaldococcus jannaschii DSM 2661 | uracil + double-stranded oligonucleotide with abasic site | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.2.27 | ? | x * 24000, SDS-PAGE | Methanocaldococcus jannaschii |
3.2.2.27 | More | UDG contains a helix-hairpin-helix motif and Gly/Pro rich loop in the GPD region important for catalytic activity | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.2.27 | MjUDG | - |
Methanocaldococcus jannaschii |
3.2.2.27 | UDG | - |
Methanocaldococcus jannaschii |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 50 | - |
assay at | Methanocaldococcus jannaschii |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.2.27 | 6 | - |
assay at | Methanocaldococcus jannaschii |