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Literature summary extracted from

  • Sahm, H.; Schuette, H.; Kula, M.R.
    3-Hexulose-phosphate synthase from Methylomonas M15 (1982), Methods Enzymol., 90, 319-323.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 1.1
-
 formaldehyde in 300 mM Tris-HCI buffer (pH 7.5), at 30°C Methylomonas sp.
4.1.2.43 1.6
-
 D-ribulose 5-phosphate in 300 mM Tris-HCI buffer (pH 7.5), at 30°C Methylomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 22000
-
 2 * 22000, SDS-PAGE Methylomonas sp.
4.1.2.43 40000
-
 gel filtration Methylomonas sp.
4.1.2.43 43000
-
 sedimentation equilibrium method Methylomonas sp.

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Methylomonas sp.
-
-
-
4.1.2.43 Methylomonas sp. M15 / DSM 580
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 DEAE-cellulose column chromatography, DEAE-Sephadex A-50 gel filtration, and Sephadex G-75 gel filtration Methylomonas sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 4.5
-
 crude extract, at 30°C Methylomonas sp.
4.1.2.43 66.5
-
 after 14.8fold purification, at 30°C Methylomonas sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde
-
 Methylomonas sp. M15 / DSM 580 D-arabino-hex-3-ulose 6-phosphate
-
 ?
4.1.2.43 additional information no detectable activity with D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 1-phosphate, D-glucose 6-phosphate, lithium hydroxypyruvate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, D-ribose 5-phosphate, and D-xylose 5-phosphate Methylomonas sp. ?
-
 ?
4.1.2.43 additional information no detectable activity with D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 1-phosphate, D-glucose 6-phosphate, lithium hydroxypyruvate, glyceraldehyde 3-phosphate, dihydroxyacetone phosphate, D-ribose 5-phosphate, and D-xylose 5-phosphate Methylomonas sp. M15 / DSM 580 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homodimer 2 * 22000, SDS-PAGE Methylomonas sp.

Synonyms

EC Number Synonyms Comment Organism
4.1.2.43 3-hexulose-phosphate synthase
-
 Methylomonas sp.
4.1.2.43 HPS
-
 Methylomonas sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 7.5 8
-
 Methylomonas sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.43 6 8 remains stable at pH 6.0-8.0 at 30°C for 1 h, enzyme stability decreases at pH values below 6.0 Methylomonas sp.