Literature summary extracted from
Chevalier, C.; Huntzinger, E.; Fechter, P.; Boisset, S.; Vandenesch, F.; Romby, P.; Geissmann, T.
Staphylococcus aureus endoribonuclease III purification and properties (2008), Methods Enzymol., 447, 309-327.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
3.1.26.3 |
overexpressed in Escherichia coli |
Staphylococcus aureus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
3.1.26.3 |
additional information |
rnc mutant (obtained by homologous recombination) is viable. Deletion of the rnc gene in Staphylococcus aureus does not affect cell growth in rich medium |
Staphylococcus aureus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
3.1.26.3 |
Ca2+ |
recombinant Sa-RNase III is inhibited by Ca2+ |
Staphylococcus aureus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
3.1.26.3 |
Mg2+ |
recombinant Sa-RNase III requires MgCl2 for activity |
Staphylococcus aureus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
3.1.26.3 |
27900 |
- |
homodimer of 243 amino acid polypeptides |
Staphylococcus aureus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
3.1.26.3 |
Staphylococcus aureus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
3.1.26.3 |
- |
Staphylococcus aureus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
3.1.26.3 |
dsRNA + H2O |
process double-stranded RNAs consisting of two turns of the RNA helix. Although the enzyme plays a role in ribosomal RNA processing and gene regulation, enzyme is not essential for cell growth but regulates virulence gene expression |
Staphylococcus aureus |
mature RNA |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
3.1.26.3 |
homodimer |
- |
Staphylococcus aureus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
3.1.26.3 |
endoribonuclease III |
- |
Staphylococcus aureus |
3.1.26.3 |
Sa-RNase III |
- |
Staphylococcus aureus |