EC Number | Cloned (Comment) | Organism |
---|---|---|
3.1.26.3 | expressed in Escherichia coli BLR(DE3) cells | Escherichia coli |
3.1.26.3 | expression in Escherichia coli BL21(DE3)rnc105,recA | Aquifex aeolicus |
3.1.26.3 | expression in Escherichia coli BL21(DE3)rnc105,recA | Thermotoga maritima |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.1.26.3 | - |
Thermotoga maritima |
3.1.26.3 | Aquifex aeolicus RNase III also has been cocrystallized with dsRNA or specific hairpin substrates | Aquifex aeolicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.26.3 | Aquifex aeolicus | O67082 | - |
- |
3.1.26.3 | Escherichia coli | - |
- |
- |
3.1.26.3 | Thermotoga maritima | Q9X0I6 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.26.3 | affinity chromatography. RNase III heterodimers also can be purified from the inclusion body | Escherichia coli |
3.1.26.3 | Ni2+ affinity column | Aquifex aeolicus |
3.1.26.3 | Ni2+ affinity column | Thermotoga maritima |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.26.3 | double-stranded RNA + H2O | - |
Escherichia coli | 5'-phosphooligonucleotides | - |
? | |
3.1.26.3 | double-stranded RNA + H2O | - |
Aquifex aeolicus | 5'-phosphooligonucleotides | - |
? | |
3.1.26.3 | double-stranded RNA + H2O | - |
Thermotoga maritima | 5'-phosphooligonucleotides | - |
? | |
3.1.26.3 | additional information | cleave internally 32P-labeled R1.1(WC) RNA | Aquifex aeolicus | ? | - |
? | |
3.1.26.3 | additional information | cleaves internally 32P-labeled R1.1(WC) RNA | Escherichia coli | ? | - |
? | |
3.1.26.3 | additional information | cleaves internally 32P-labeled R1.1(WC) RNA | Thermotoga maritima | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.26.3 | heterodimer | - |
Aquifex aeolicus |
3.1.26.3 | heterodimer | preparation of artificial heterodimers of RNase III, which are providing new insight on the subunit and domain interactions involved in dsRNA recognition and cleavage | Escherichia coli |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.26.3 | Aa-RNase III | - |
Aquifex aeolicus |
3.1.26.3 | ribonuclease III | - |
Escherichia coli |
3.1.26.3 | ribonuclease III | - |
Aquifex aeolicus |
3.1.26.3 | ribonuclease III | - |
Thermotoga maritima |
3.1.26.3 | RNase III | - |
Escherichia coli |
3.1.26.3 | RNase III | - |
Aquifex aeolicus |
3.1.26.3 | RNase III | - |
Thermotoga maritima |
3.1.26.3 | Tm-RNase III | - |
Thermotoga maritima |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.26.3 | additional information | - |
hyperthermophilic bacterium | Aquifex aeolicus |
3.1.26.3 | additional information | - |
hyperthermophilic bacterium | Thermotoga maritima |