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Literature summary extracted from

  • Meng, W.; Nicholson, R.H.; Nathania, L.; Pertzev, A.V.; Nicholson, A.W.
    New approaches to understanding double-stranded RNA processing by ribonuclease III purification and assays of homodimeric and heterodimeric forms of RNase III from bacterial extremophiles and mesophiles (2008), Methods Enzymol., 447, 119-129.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.26.3 expressed in Escherichia coli BLR(DE3) cells Escherichia coli
3.1.26.3 expression in Escherichia coli BL21(DE3)rnc105,recA Aquifex aeolicus
3.1.26.3 expression in Escherichia coli BL21(DE3)rnc105,recA Thermotoga maritima

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.26.3
-
Thermotoga maritima
3.1.26.3 Aquifex aeolicus RNase III also has been cocrystallized with dsRNA or specific hairpin substrates Aquifex aeolicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.3 Aquifex aeolicus O67082
-
-
3.1.26.3 Escherichia coli
-
-
-
3.1.26.3 Thermotoga maritima Q9X0I6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.3 affinity chromatography. RNase III heterodimers also can be purified from the inclusion body Escherichia coli
3.1.26.3 Ni2+ affinity column Aquifex aeolicus
3.1.26.3 Ni2+ affinity column Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.3 double-stranded RNA + H2O
-
Escherichia coli 5'-phosphooligonucleotides
-
?
3.1.26.3 double-stranded RNA + H2O
-
Aquifex aeolicus 5'-phosphooligonucleotides
-
?
3.1.26.3 double-stranded RNA + H2O
-
Thermotoga maritima 5'-phosphooligonucleotides
-
?
3.1.26.3 additional information cleave internally 32P-labeled R1.1(WC) RNA Aquifex aeolicus ?
-
?
3.1.26.3 additional information cleaves internally 32P-labeled R1.1(WC) RNA Escherichia coli ?
-
?
3.1.26.3 additional information cleaves internally 32P-labeled R1.1(WC) RNA Thermotoga maritima ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.26.3 heterodimer
-
Aquifex aeolicus
3.1.26.3 heterodimer preparation of artificial heterodimers of RNase III, which are providing new insight on the subunit and domain interactions involved in dsRNA recognition and cleavage Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
3.1.26.3 Aa-RNase III
-
Aquifex aeolicus
3.1.26.3 ribonuclease III
-
Escherichia coli
3.1.26.3 ribonuclease III
-
Aquifex aeolicus
3.1.26.3 ribonuclease III
-
Thermotoga maritima
3.1.26.3 RNase III
-
Escherichia coli
3.1.26.3 RNase III
-
Aquifex aeolicus
3.1.26.3 RNase III
-
Thermotoga maritima
3.1.26.3 Tm-RNase III
-
Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.26.3 additional information
-
hyperthermophilic bacterium Aquifex aeolicus
3.1.26.3 additional information
-
hyperthermophilic bacterium Thermotoga maritima