Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kato, N.
    3-Hexulose-6-phosphate synthase from Mycobacterium gastri MB19 (1990), Methods Enzymol., 188, 397-401.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.43 EDTA the enzyme is completely inhibited by the presence of 2 mM EDTA Mycobacterium gastri
4.1.2.43 additional information the enzyme is inactivated irreversibly on dialysis against buffer without Mg2+ Mycobacterium gastri
4.1.2.43 o-phenanthroline the enzyme is completely inhibited by the presence of 2 mM o-phenanthroline Mycobacterium gastri
4.1.2.43 Tiron the enzyme is completely inhibited by the presence of 2 mM tiron Mycobacterium gastri

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.43 1.4
-
formaldehyde in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C Mycobacterium gastri
4.1.2.43 4.3
-
glycolaldehyde in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C Mycobacterium gastri
4.1.2.43 5.7
-
methylglyoxal in 500 mM potassium phosphate buffer, at pH 7.5, at 30°C Mycobacterium gastri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.2.43 Mg2+ essential requirement for Mg2+ Mycobacterium gastri
4.1.2.43 Mn2+ essential requirement for Mn2+ Mycobacterium gastri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.43 43000
-
TSK gel G-3000SW chromatography Mycobacterium gastri
4.1.2.43 48000
-
2 * 48000, SDS-PAGE Mycobacterium gastri

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.43 Mycobacterium gastri
-
-
-
4.1.2.43 Mycobacterium gastri MB19
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.43 phenyl-Sepharose column chromatography, and DEAE-Sephacel column chromatography Mycobacterium gastri

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.43 3.2
-
cell free extract, at 30°C Mycobacterium gastri
4.1.2.43 74.2
-
after 23fold purification, at 30°C Mycobacterium gastri

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.43 D-ribulose 5-phosphate + formaldehyde the enzyme is highly specific for D-ribulose 5-phosphate as an acceptor of aldehydes Mycobacterium gastri D-arabino-hex-3-ulose 6-phosphate
-
?
4.1.2.43 D-ribulose 5-phosphate + formaldehyde the enzyme is highly specific for D-ribulose 5-phosphate as an acceptor of aldehydes Mycobacterium gastri MB19 D-arabino-hex-3-ulose 6-phosphate
-
?
4.1.2.43 D-ribulose 5-phosphate + glycolaldehyde
-
Mycobacterium gastri ?
-
?
4.1.2.43 D-ribulose 5-phosphate + glycolaldehyde
-
Mycobacterium gastri MB19 ?
-
?
4.1.2.43 D-ribulose 5-phosphate + methylglyoxal
-
Mycobacterium gastri ?
-
?
4.1.2.43 D-ribulose 5-phosphate + methylglyoxal
-
Mycobacterium gastri MB19 ?
-
?
4.1.2.43 additional information no detectable activity with D-ribulose 1,5-bisphosphate, D-ribose 5-phosphate, D-erythrose 4-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, and D-xyluloSe 5-phosphate Mycobacterium gastri ?
-
?
4.1.2.43 additional information no detectable activity with D-ribulose 1,5-bisphosphate, D-ribose 5-phosphate, D-erythrose 4-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, D-glucose 6-phosphate, D-glucose 1-phosphate, and D-xyluloSe 5-phosphate Mycobacterium gastri MB19 ?
-
?

Subunits

EC Number Subunits Comment Organism
4.1.2.43 homodimer 2 * 48000, SDS-PAGE Mycobacterium gastri

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.2.43 6.5 7.5 remains stable in the pH range of 6.5-7.5 at 30°C for 6 h Mycobacterium gastri

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.43 7.5 8 in potassium phosphate buffer Mycobacterium gastri