Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Munoz-Bertomeu, J.; Ros, R.; Arrillaga, I.; Segura, J.
    Expression of spearmint limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves (2008), Metab. Eng., 10, 166-177.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.3.16 agriculture putative production of transgenic aromatic plants overexpressing monoterpene synthases Mentha spicata
4.2.3.16 industry limonene is an aromatic component in numerous products like beverages and cosmetics Mentha spicata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.16 Agrobacterium-mediated transformation and regeneration of spike lavender is carried out and the transgenic Lavandula latifolia plants express the limonene synthase gene from Mentha spicata. Expression of limonene synthase in transgenic spike lavender results in an altered monoterpene composition in developing leaves, but does not consistently affect essential oil profile in pooled leaves and flowers of transgenic generation T0. Mentha spicata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.3.16 plastid
-
 Mentha spicata 9536
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.16 geranyl diphosphate Mentha spicata step 3 and 4 of monoterpene biosynthesis, geranyl diphosphate is first ionized and isomerized to produce linalyl diphosphate, which either produces the acyclic monoterpenes or the alpha-terpinyl cation, the universal intermediate for cyclic monoterpenes and transformation of the parent structures to various derivatives limonene + diphosphate limonene is a cyclic monoterpene, minor constituent of the spike lavender oil ?
4.2.3.16 additional information Mentha spicata overexpression of the LS does not alter the photosynthetic pigment content of spike lavender leaves ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.16 Mentha spicata
-
 transgenic spike lavender plants expressing the limonene synthase gene from spearmint
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.16 flower
-
 Mentha spicata
-
4.2.3.16 leaf the first two pairs of leaves from 40-day-old seedlings are used as primary explants for transformation Mentha spicata
-
4.2.3.16 seed from natural populations, germinated under sterile conditions Mentha spicata
-
4.2.3.16 stem
-
 Mentha spicata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.3.16 additional information
-
 increase in the expression of the spearmint LS cDNA at the different leaf developmental stages is accompanied with increases in the limonene content of transgenic spike lavender lines Mentha spicata
4.2.3.16 additional information
-
 leaves of transgenic plants contain increased limonene contents (more than 450% increase compared to controls) that correlates with the highest transcript accumulation of the LS gene. Mentha spicata
4.2.3.16 additional information
-
 the effect of the LS expression on oil composition decreases with leaf age as compared to the control Mentha spicata
4.2.3.16 additional information
-
 transgenic T0 spike lavender lines overexpressing MsLS show quantitative and qualitative alterations in their terpene profiles, particularly increased amounts of limonene as compared to controls (from 19.05% to 53.97% and from 12.50% to 83.33% in pooled leaves and flowers, respectively). The levels of other monoterpene pathway components are not consistently altered in either leaf or flower oils Mentha spicata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.16 geranyl diphosphate step 3 and 4 of monoterpene biosynthesis, geranyl diphosphate is first ionized and isomerized to produce linalyl diphosphate, which either produces the acyclic monoterpenes or the alpha-terpinyl cation, the universal intermediate for cyclic monoterpenes and transformation of the parent structures to various derivatives Mentha spicata limonene + diphosphate limonene is a cyclic monoterpene, minor constituent of the spike lavender oil ?
4.2.3.16 additional information overexpression of the LS does not alter the photosynthetic pigment content of spike lavender leaves Mentha spicata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.16 limonene synthase
-
 Mentha spicata
4.2.3.16 LS
-
 Mentha spicata
4.2.3.16 MsLS
-
 Mentha spicata