Any feedback?
Literature summary extracted from
- Cloning and Characterization of Alginate Lyase from a Marine Bacterium Streptomyces sp. ALG-5 (2009), Mar. Biotechnol., 11, 10-16.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.2.3 | expressed as a His-tagged fusion protein in Escherichia coli | Streptomyces sp. |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.3 | 27500 | - |
SDS-PAGE | Streptomyces sp. |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.2.3 | Streptomyces sp. | A7LFP7 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.2.3 | using Ni-Sepharose affinity chromatography | Streptomyces sp. |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.2.3 | alginate | enzyme lyase appears to be poly-guluronate lyase degrading poly-G block preferentially than poly-M block. The degraded products are determined to be di-, tri-, tetra- and pentasaccharides | Streptomyces sp. | unsaturated algino-oligosaccharides | - |
? |  |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.2.2.3 | ALG-5 | - |
Streptomyces sp. |
| 4.2.2.3 | alginate lyase | - |
Streptomyces sp. |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.3 | 30 | - |
assay at | Streptomyces sp. |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.2.3 | 7 | - |
assay at | Streptomyces sp. |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
