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Literature summary extracted from

  • Coulson-Thomas, Y.M.; Coulson-Thomas, V.J.; Filippo, T.R.; Mortara, R.A.; da Silveira, R.B.; Nader, H.B.; Porcionatto, M.A.
    Adult bone marrow-derived mononuclear cells expressing chondroitinase AC transplanted into CNS injury sites promote local brain chondroitin sulphate degradation (2008), J. Neurosci. Methods, 171, 19-29.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.2.2.5 medicine bone marrow-derived mononuclear cells, transfected with our construct and transplanted into CNS, could be a potential tool for studying an alternative chondroitinase AC delivery method Pedobacter heparinus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.2.11 expressed in Escherichia coli Agarivorans sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.2.2.5 additional information a vector is engineered containing the gene for Flavobacterium heparinum chondroitinase AC for expression in adult bone marrow-derived cells which are then transplanted into an injury site in the CNS. Expression and secretion of active chondroitinase AC is observed in vitro using transfected Chinese hamster ovarian and gliosarcoma cells and in vivo by immunohistochemistry analysis which show degraded chondroitin sulfate coinciding with the location of transfected bone marrow-derived cells. Immunolabelling of the axonal growth-associated protein GAP-43 is observed in vivo and coincides with the location of degraded chondroitin sulfate Pedobacter heparinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.11 (NH4)2SO4 relative activity: 409% Agarivorans sp.
4.2.2.11 CaCl2 relative activity: 76% Agarivorans sp.
4.2.2.11 CH3COO- NH4+ relative activity: 298% Agarivorans sp.
4.2.2.11 CH3COONa relative activity: 233% Agarivorans sp.
4.2.2.11 CoCl2 relative activity: 102% Agarivorans sp.
4.2.2.11 CuSO4 relative activity: 93% Agarivorans sp.
4.2.2.11 EDTA relative activity: 14% Agarivorans sp.
4.2.2.11 EGTA relative activity: 14% Agarivorans sp.
4.2.2.11 HgCl2 relative activity: 29% Agarivorans sp.
4.2.2.11 K2HPO4 relative activity: 439% Agarivorans sp.
4.2.2.11 KCl relative activity: 521% Agarivorans sp.
4.2.2.11 MgCl2 relative activity: 101% Agarivorans sp.
4.2.2.11 MnCl2 relative activity: 72% Agarivorans sp.
4.2.2.11 Na+ activity is increased to 1.8fold by adding 0.2 M NaCl Agarivorans sp.
4.2.2.11 NaBr relative activity: 712% Agarivorans sp.
4.2.2.11 NaCl relative activity: 823% Agarivorans sp.
4.2.2.11 NaF relative activity: 419% Agarivorans sp.
4.2.2.11 NH4Cl relative activity: 549% Agarivorans sp.
4.2.2.11 NiCl2 relative activity: 106% Agarivorans sp.
4.2.2.11 PdCl2 relative activity: 5% Agarivorans sp.
4.2.2.11 SrCl2 relative activity: 54% Agarivorans sp.
4.2.2.11 ZnCl2 relative activity: 111% Agarivorans sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.11 31000
-
 SDS-PAGE Agarivorans sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.5 Pedobacter heparinus Q59288
-
-
4.2.2.11 Agarivorans sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.11 using a SuperQ Toyopearl column and Butyl Toyopearl column Agarivorans sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.2.11 alginate A1m preferably degrades the heteropolymeric MG and G blocks (1,4 linked alpha-L-guluronic acid) to the M block (beta-D-mannuronic acid). The relative activities for alginate, MG, G, and M blocks are 100%, 131.7%, 83.3%, and 27.3%, respectively Agarivorans sp. ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.2.5 chondroitinase AC
-
 Pedobacter heparinus
4.2.2.11 A1m
-
 Agarivorans sp.
4.2.2.11 alginate lyase
-
 Agarivorans sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.11 30
-
-
 Agarivorans sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.11 30 50 enzyme is rapidly inactivated upon incubation at 30-50°C for 15 min Agarivorans sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.11 10
-
-
 Agarivorans sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.11 6 9 A1m is quite stable in incubation at 30°C for 1 h between pH 6 and 9 Agarivorans sp.