Literature summary extracted from

Minagawa, A.; Ishii, R.; Takaku, H.; Yokoyama, S.; Nashimoto, M.
The flexible arm of tRNase Z is not essential for pre-tRNA binding but affects cleavage site selection (2008), J. Mol. Biol., 381, 289-299.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.1.26.11	His-tag	Bacillus subtilis
3.1.26.11	His-tag	Escherichia coli
3.1.26.11	His-tag	Thermoplasma acidophilum
3.1.26.11	His-tag	Thermotoga maritima
3.1.26.11	His-tag	Pyrobaculum aerophilum

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.1.26.11	K162L	residue at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	K162L/R166L/K169L	residues at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	K162R	residue at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	K169L	residue at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	K169R	residue at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	Q44S	very low activity	Pyrobaculum aerophilum
3.1.26.11	Q44S/Q46T	double mutant, cleavage of human pre-tRNA(Arg) after U75, very inefficiently	Escherichia coli
3.1.26.11	Q45S/Q47T	very low activity	Thermoplasma acidophilum
3.1.26.11	R166L	residue at the flexible arm of tRNase Z	Thermotoga maritima
3.1.26.11	S31Q	cleavage site selection affected	Thermotoga maritima
3.1.26.11	S31Q/T33Q	cleavage site analyzed	Thermotoga maritima
3.1.26.11	T33Q	cleavage site selection affected	Thermotoga maritima

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.26.11	Bacillus subtilis	-	-	-
3.1.26.11	Escherichia coli	-	-	-
3.1.26.11	Pyrobaculum aerophilum	-	-	-
3.1.26.11	Thermoplasma acidophilum	-	-	-
3.1.26.11	Thermotoga maritima	-	-	-

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.26.11	tRNase Z	-	Bacillus subtilis
3.1.26.11	tRNase Z	-	Escherichia coli
3.1.26.11	tRNase Z	-	Thermoplasma acidophilum
3.1.26.11	tRNase Z	-	Thermotoga maritima
3.1.26.11	tRNase Z	-	Pyrobaculum aerophilum

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Release 2023.1 (January 2023)