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Literature summary extracted from

  • Syed, D.G.; Agasar, D.; Pandey, A.
    Production and partial purification of alpha-amylase from a novel isolate Streptomyces gulbargensis (2009), J. Ind. Microbiol. Biotechnol., 36, 189-194.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.1 industry the alkaphilic nature of the enzyme with its stability is a interesting feature for possible industrial application Streptomyces gulbargensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.1 Ag+ 1 mM, 37°C, 30 min, pH 6.5, 36% relative activity Streptomyces gulbargensis
3.2.1.1 Ba2+ 1 mM, 37°C, 30 min, pH 6.5, 29% relative activity Streptomyces gulbargensis
3.2.1.1 Co2+ 1 mM, 37°C, 30 min, pH 6.5, 23% relative activity Streptomyces gulbargensis
3.2.1.1 Cs2+ 1 mM, 37°C, 30 min, pH 6.5, 89% relative activity Streptomyces gulbargensis
3.2.1.1 Fe2+ 1 mM, 37°C, 30 min, pH 6.5, 81% relative activity Streptomyces gulbargensis
3.2.1.1 Fe3+ 1 mM, 37°C, 30 min, pH 6.5, 57% relative activity Streptomyces gulbargensis
3.2.1.1 Hg2+ 1 mM, 37°C, 30 min, pH 6.5, 44% relative activity Streptomyces gulbargensis
3.2.1.1 Zn2+ 1 mM, 37°C, 30 min, pH 6.5, 83% relative activity Streptomyces gulbargensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.1 Ca2+ 1 mM, 37°C, 30 min, pH 6.5, 148% relative activity Streptomyces gulbargensis
3.2.1.1 Cu2+ 1 mM, 37°C, 30 min, pH 6.5, 100% relative activity Streptomyces gulbargensis
3.2.1.1 Mg2+ 1 mM, 37°C, 30 min, pH 6.5, 98% relative activity Streptomyces gulbargensis
3.2.1.1 Mn2+ 1 mM, 37°C, 30 min, pH 6.5, 96% relative activity Streptomyces gulbargensis
3.2.1.1 Ni2+ 1 mM, 37°C, 30 min, pH 6.5, 100% relative activity Streptomyces gulbargensis
3.2.1.1 Pb2+ 1 mM, 37°C, 30 min, pH 6.5, 91% relative activity Streptomyces gulbargensis
3.2.1.1 Sr2+ 1 mM, 37°C, 30 min, pH 6.5, 92% relative activity Streptomyces gulbargensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.1 55000
-
 SDS-PAGE Streptomyces gulbargensis
3.2.1.1 57900
-
 gel filtration Streptomyces gulbargensis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.1 Streptomyces gulbargensis
-
 DAS 131, alkali-thermotolerant strain
-
3.2.1.1 Streptomyces gulbargensis DAS 131
-
 DAS 131, alkali-thermotolerant strain
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.1
-
 Streptomyces gulbargensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.1 culture condition:peptone-grown cell a good nitrogen source Streptomyces gulbargensis
-
3.2.1.1 culture condition:starch-grown cell a good carbon source, optimum yield is obtained, when the cells are grown in a minimal medium containing 1% (w/v) starch Streptomyces gulbargensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.1 additional information maltose is not hydrolyzed by the alpha-amylase Streptomyces gulbargensis ?
-
 ?
3.2.1.1 additional information maltose is not hydrolyzed by the alpha-amylase Streptomyces gulbargensis DAS 131 ?
-
 ?
3.2.1.1 starch + H2O pH 6.5, 37°C Streptomyces gulbargensis maltose + maltotriose the main products ?
3.2.1.1 starch + H2O pH 6.5, 37°C Streptomyces gulbargensis DAS 131 maltose + maltotriose the main products ?

Subunits

EC Number Subunits Comment Organism
3.2.1.1 monomer 1 * 55000, SDS-PAGE Streptomyces gulbargensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.1 37
-
 assay at Streptomyces gulbargensis
3.2.1.1 45
-
-
 Streptomyces gulbargensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.1 35 55 35°C: ca. 400 U/mg, 55°C: ca. 400 U/mg, temperature optimum at 45°C showing ca. 1300 U/mg protein Streptomyces gulbargensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.1 45 50 more than 40% of the activity is lost Streptomyces gulbargensis
3.2.1.1 60
-
 more than 75-85% if the activity is lost Streptomyces gulbargensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.1 6.5
-
 assay at Streptomyces gulbargensis
3.2.1.1 9
-
 remains active at very high alkaline pH (8.5-11), a characteristic that distinguishes it from most of the other alpha-amylases from bacterial sources Streptomyces gulbargensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.1 11
-
 relatively stable in alkaline condition with at least 70% of the activity at pH 9.0 Streptomyces gulbargensis