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Literature summary extracted from

  • Gunashree, B.S.; Venkateswaran, G.
    Effect of different cultural conditions for phytase production by Aspergillus niger CFR 335 in submerged and solid-state fermentations (2008), J. Ind. Microbiol. Biotechnol., 35, 1587-1596.
    View publication on PubMed

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.8 Aspergillus niger
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the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
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Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.8 a 1.5fold higher enzyme yield in the presence of sucrose in both submerged and solid-state fermentations, while peptone is found to be a favorable nitrogen source for submerged fermentations. NaH2PO4 favors 34% higher enzyme yield than the control, which is followed by 19% higher activity in KH2PO4 in solid-state fermentation at 0.015% w/v. The addition of Tween-20 in submerged fermentations shows a maximum yield of 12.6 U/ml while, SDS suppresses the growth of the fungus. None of the surfactants favors the enzyme yield in solid-state fermentation. CaCl2 is extensively efficient in stimulating more than 55% higher phytase production in submerged fermentation at 0.01% v/v. In solid-state fermentation, none of the metal salts stimulates phytase production Aspergillus niger

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.8 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Aspergillus niger ? + phosphate
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Synonyms

EC Number Synonyms Comment Organism
3.1.3.8 phytase
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Aspergillus niger

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.8 40
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assay at Aspergillus niger

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.8 4.5
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assay at Aspergillus niger