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Literature summary extracted from

  • Kagami, O.; Shindo, K.; Kyojima, A.; Takeda, K.; Ikenaga, H.; Furukawa, K.; Misawa, N.
    Protein engineering on biphenyl dioxygenase for conferring activity to convert 7-hydroxyflavone and 5,7-dihydroxyflavone (chrysin) (2008), J. Biosci. Bioeng., 106, 121-127.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.18 A central part (amino acid position 268-397 of 458 residues) of the biphenyl dioxygenase large alpha subunit, BphA1, from Pseudomonas pseudoalcaligenes (strain KF707) is exchanged with the corresponding part of BphA1 from Pseudomonas putida strain KF715, to construct hybrid BphA1, BphA1 (amino acid 715-707). When expressed in Escherichia coli together with the bphA2A3A4BC genes from strain KF707, this enzyme is shown to possess activity for degrading both 1-phenylnaphthalene and 2-phenylnaphthalene. Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.18 Pseudomonas putida
-
 and strain KF751
-
1.14.12.18 Pseudomonas putida KF715
-
 and strain KF751
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.12.18 cell culture
-
 Pseudomonas putida
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.18 5,7-dihydroxyflavone + NADH + H+ + O2 BphA1 (1-22) and BphA1 (2-2), expression of the mutants in together with bphA2A3A4B from strain KF707 Pseudomonas putida 2-(2,3-dihydroxyphenyl)-5,7-dihydroxy-chromen-4-one + NAD+
-
 ?
1.14.12.18 5,7-dihydroxyflavone + NADH + H+ + O2 BphA1 (1-22) and BphA1 (2-2), expression of the mutants in together with bphA2A3A4B from strain KF707 Pseudomonas putida KF715 2-(2,3-dihydroxyphenyl)-5,7-dihydroxy-chromen-4-one + NAD+
-
 ?
1.14.12.18 7-hydroxyflavone + NADH + H+ + O2 BphA1 (1-22) and BphA1 (2-2), expression of the mutants together with bphA2A3A4B from strain KF707 Pseudomonas putida 2-(2,3-dihydroxyphenyl)-7-hydroxy-chromen-4-one + NAD+
-
 ?
1.14.12.18 7-hydroxyflavone + NADH + H+ + O2 BphA1 (1-22) and BphA1 (2-2), expression of the mutants together with bphA2A3A4B from strain KF707 Pseudomonas putida KF715 2-(2,3-dihydroxyphenyl)-7-hydroxy-chromen-4-one + NAD+
-
 ?
1.14.12.18 additional information trans-chalcone is converted into 3-(2,3-dihydroxyphenyl)-1-phenylpropan-1-one and further into 1,3-bis-(2,3-dihydroxyphenyl)-propan-1-one Pseudomonas putida ?
-
 ?
1.14.12.18 additional information trans-chalcone is converted into 3-(2,3-dihydroxyphenyl)-1-phenylpropan-1-one and further into 1,3-bis-(2,3-dihydroxyphenyl)-propan-1-one Pseudomonas putida KF715 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.12.18 heterotetramer BphA1 (iron-sulfur protein large alpha subunit), BphA2 (iron-sulfur protein small beta subunit), BphA3 (ferredoxin) and BphA4 (ferredoxin reductase). Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.14.12.18 biphenyl dioxygenase
-
 Pseudomonas putida
1.14.12.18 BphA1 large alpha subunit Pseudomonas putida