EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.211 | synthesis | suitable catalyst for practical syntheses of oligosaccharides | Lachnoclostridium phytofermentans |
2.4.1.247 | synthesis | suitable catalyst for practical syntheses of oligosaccharides | Lachnoclostridium phytofermentans |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.211 | expressed in Escherichia coli BL21(DE3) | Lachnoclostridium phytofermentans |
2.4.1.247 | expressed in Escherichia coli BL21(DE3) | Lachnoclostridium phytofermentans |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.211 | 1.9 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 2.8 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 3.3 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 3.4 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 4.5 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 8 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 10 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 2.4 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 7.9 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 78 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 220 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 290 | - |
2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 460 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 610 | - |
D-galactose | - |
Lachnoclostridium phytofermentans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.211 | cytosol | proteins are predicted not to possess N-terminal signal peptides | Lachnoclostridium phytofermentans | 5829 | - |
2.4.1.247 | cytosol | proteins are predicted not to possess N-terminal signal peptides | Lachnoclostridium phytofermentans | 5829 | - |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 80000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
2.4.1.211 | 84860 | - |
calculated | Lachnoclostridium phytofermentans |
2.4.1.211 | 84940 | - |
calculated | Lachnoclostridium phytofermentans |
2.4.1.247 | 80000 | - |
SDS-PAGE | Lachnoclostridium phytofermentans |
2.4.1.247 | 84230 | - |
calculated | Lachnoclostridium phytofermentans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.211 | Lachnoclostridium phytofermentans | A9KIW5 | bacterium possesses three GalHexNAcP homologous genes, cphy0577 gene; anaerobic cellulolytic bacterium | - |
2.4.1.211 | Lachnoclostridium phytofermentans | A9KQ75 | bacterium possesses three GalHexNAcP homologous genes, Cphy3030 gene; anaerobic cellulolytic bacterium | - |
2.4.1.247 | Lachnoclostridium phytofermentans | A9KHK4 | bacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.211 | - |
Lachnoclostridium phytofermentans |
2.4.1.247 | - |
Lachnoclostridium phytofermentans |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.211 | galacto-N-biose + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-galactosamine | - |
r | |
2.4.1.211 | lacto-N-biose I + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
r | |
2.4.1.211 | lacto-N-biose I + phosphate | - |
Lachnoclostridium phytofermentans | alpha-D-galactose 1-phosphate + N-acetyl-D-glucosamine | - |
? | |
2.4.1.211 | additional information | Cphy0577 protein does not show activity on either L-Rha, D-Glc, or D-Gal | Lachnoclostridium phytofermentans | ? | - |
? | |
2.4.1.211 | additional information | Cphy3030 protein does not show activity on either L-Rha, D-Glc, or D-Gal | Lachnoclostridium phytofermentans | ? | - |
? | |
2.4.1.247 | 2-deoxy-D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate | - |
r | |
2.4.1.247 | alpha-D-galactose 1-phosphate + L-rhamnose | highest activity | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-rhamnose + phosphate | - |
r | |
2.4.1.247 | D-galactose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-galactose + phosphate | - |
r | |
2.4.1.247 | D-glucose + alpha-D-galactose 1-phosphate | when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides | Lachnoclostridium phytofermentans | D-galactosyl-beta-1,3-D-glucose + phosphate | - |
r | |
2.4.1.247 | L-lyxose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | beta-D-galactosyl-(1->4)-L-lyxose + phosphate | - |
r | |
2.4.1.247 | L-mannose + alpha-D-galactose 1-phosphate | - |
Lachnoclostridium phytofermentans | D-galactosyl-beta-1,4-L-mannose + phosphate | - |
r | |
2.4.1.247 | additional information | phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP | Lachnoclostridium phytofermentans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.211 | cphy0577 protein | - |
Lachnoclostridium phytofermentans |
2.4.1.211 | cphy3030 protein | - |
Lachnoclostridium phytofermentans |
2.4.1.211 | D-galactosyl-beta1,3-N-acetyl-D-hexosamine phosphorylase | - |
Lachnoclostridium phytofermentans |
2.4.1.211 | GalHexNAcP | - |
Lachnoclostridium phytofermentans |
2.4.1.211 | GNB/LNB phosphorylase | - |
Lachnoclostridium phytofermentans |
2.4.1.247 | cphy1920 protein | - |
Lachnoclostridium phytofermentans |
2.4.1.247 | D-galactosyl-beta-1,4-L-rhamnose phosphorylase | - |
Lachnoclostridium phytofermentans |
2.4.1.247 | GalRhaP | - |
Lachnoclostridium phytofermentans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 30 | - |
protein is thermostable up to 30°C | Lachnoclostridium phytofermentans |
2.4.1.211 | 45 | - |
protein is thermostable up to 45°C | Lachnoclostridium phytofermentans |
2.4.1.247 | 37 | - |
protein is thermostable up to 37°C | Lachnoclostridium phytofermentans |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.211 | 34 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 39 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 39 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 40 | - |
lacto-N-biose I | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 42 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 48 | - |
N-acetyl-D-galactosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 49 | - |
N-acetyl-D-glucosamine | - |
Lachnoclostridium phytofermentans | |
2.4.1.211 | 50 | - |
galacto-N-biose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 2 | 8 | 2-deoxy-D-glucose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 24 | - |
D-galactose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 45 | - |
D-galactosyl-beta-1,4-L-rhamnose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 74 | - |
L-rhamnose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 85 | - |
D-glucose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 120 | - |
L-Lyxose | - |
Lachnoclostridium phytofermentans | |
2.4.1.247 | 170 | - |
L-Mannose | - |
Lachnoclostridium phytofermentans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.211 | 6 | 7 | optimum activity at neutral pH | Lachnoclostridium phytofermentans |
2.4.1.247 | 6 | 7 | optimum activity at neutral pH | Lachnoclostridium phytofermentans |