Literature summary extracted from
Sundaram, S.; Rathinasabapathi, B.; Ma, L.Q.; Rosen, B.P.
An arsenate-activated glutaredoxin from the arsenic hyperaccumulator fern Pteris vittata L. regulates intracellular arsenite (2008), J. Biol. Chem., 283, 6095-6101.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.20.4.1 |
arsenate |
10 mM increases enzyme activity by 1.6fold |
Pteris vittata |
|
Application
EC Number |
Application |
Comment |
Organism |
---|
1.20.4.1 |
degradation |
PvGrx5 has a role in regulating intracellular arsenite levels, by either directly or indirectly modulating the aquaglyceroporin |
Pteris vittata |
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.20.4.1 |
gene Pv5-6 encoding PvGrx5 expressed in Escherichia coli strain XL-1 Blue. PvGrx5 expressed in Escherichia coli mutants in which the arsenic resistance genes of the ars operon are deleted (strain AW3110), a deletion of the gene for the ArsC arsenate reductase (strain WC3110), and a strain in which the ars operon is deleted and the gene for the GlpF aquaglyceroporin is disrupted (strain OSBR1) |
Pteris vittata |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.20.4.1 |
C67A |
loss of both GRX activity and arsenic resistance |
Pteris vittata |
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.20.4.1 |
Pteris vittata |
Q1A5X4 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.20.4.1 |
affinity purified |
Pteris vittata |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.20.4.1 |
arsenate + reduced glutaredoxin |
- |
Pteris vittata |
arsenite + oxidized glutaredoxin |
- |
? |
|
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.20.4.1 |
glutaredoxin |
- |
Pteris vittata |
1.20.4.1 |
Grx |
- |
Pteris vittata |
1.20.4.1 |
PvGRX5 |
- |
Pteris vittata |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
1.20.4.1 |
8 |
- |
- |
Pteris vittata |