EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.2.4 | additional information | The absence of the aspartate biosynthetic pathway in humans makes it a good target for new pesticides and antibiotics. | Methanocaldococcus jannaschii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.2.4 | pET-41a vector and Rosetta (DE3) Escherichia coli cells for protein expression | Methanocaldococcus jannaschii |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.2.4 | 3C20, 14% PEG4000, 0.1 M Tris, 0.8 M ammonium formate, pH 8.0, vapor diffusion, hanging drop, temperature 293 K, space group C2221, 3C1N, 0.2 M ammonium iodide, 2.2 M ammonium sulfate, pH 5.0, vapor diffusion, hanging drop, temperature 293 K, space group P212121, 3C1M, 14% PEG4000, 100 mM Tris, 800 mM ammonium formate, pH 8.0, vapor diffusion, hanging drop, temperature 293 K, space group P212121, the structure is determined under three different transition states: ternary complex with MgAMP-PNP and L-aspartate, binary complex with L-aspartate, and binary complex in the presence of its allosteric inhibitor L-threonine | Methanocaldococcus jannaschii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.4 | L-threonine | mechanism for allosteric regulation in which the domain movements induced by L-threonine binding causes displacement of the substrates from the enzyme, resulting in a relaxed, inactive conformation | Methanocaldococcus jannaschii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.2.4 | Mg2+ | - |
Methanocaldococcus jannaschii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.4 | ATP + L-aspartate | Methanocaldococcus jannaschii | L-aspartate binds to this recombinant enzyme in two different orientations | ADP + 4-phospho-L-aspartate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.4 | Methanocaldococcus jannaschii | Q57991 | Methanococcus jannaschii has only a single, monofunctional form of AK; pET-41a vector and Rosetta (DE3) Escherichia coli cells for protein expression | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.2.4 | two successive chromatography steps: first a high resolution anionic exchange resin, followed by gel filtration column, the resulting protein is shown by SDS-PAGE to be more than 99% pure, concentration to 30 mg/ml by using a 10000 molecular weight cut-off Amicon concentrator | Methanocaldococcus jannaschii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.4 | ATP + L-aspartate | L-aspartate binds to this recombinant enzyme in two different orientations | Methanocaldococcus jannaschii | ADP + 4-phospho-L-aspartate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.2.4 | tetramer | dimer of dimers | Methanocaldococcus jannaschii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.2.4 | aspartokinase | - |
Methanocaldococcus jannaschii |