EC Number | Application | Comment | Organism |
---|---|---|---|
1.2.1.26 | degradation | involved in an alternative pathway of D-glucarate metabolism | Acinetobacter baylyi |
1.2.1.26 | degradation | involved in an alternative pathway of D-glucarate metabolism | Pseudomonas putida |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.2.1.26 | expression in Escherichia coli | Pseudomonas putida |
1.2.1.26 | expression in Escherichia coli BL21 (DE3) | Acinetobacter baylyi |
4.2.1.40 | into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells | Acinetobacter baylyi |
4.2.1.41 | into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells | Acinetobacter baylyi |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.2.1.26 | additional information | alpha-KGSA dehydrogenase activity in cell extracts from the mutant DELTAACIAD0131 grown on succinate as the sole carbon source is undetectable | Acinetobacter baylyi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.26 | additional information | no inhibitory effect with alpha-ketoglutarate or glutamate | Acinetobacter baylyi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.26 | 0.014 | - |
alpha-Ketoglutaric semialdehyde | - |
Pseudomonas putida | |
1.2.1.26 | 0.015 | - |
NADP+ | - |
Acinetobacter baylyi | |
1.2.1.26 | 0.1 | - |
Glutaraldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.3 | - |
propanal | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.31 | - |
Glutaraldehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.45 | - |
succinate semialdehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.87 | - |
NAD+ | - |
Acinetobacter baylyi | |
1.2.1.26 | 1.05 | - |
propanal | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 1.66 | - |
succinate semialdehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 3.9 | - |
acetaldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
4.2.1.40 | 0.101 | - |
D-glucarate | - |
Acinetobacter baylyi | |
4.2.1.41 | 0.203 | - |
5-dehydro-4-deoxy-D-glucarate | - |
Acinetobacter baylyi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.2.1.26 | 62000 | - |
2 * 62000, SDS-PAGE | Acinetobacter baylyi |
4.2.1.40 | 34960 | - |
calculated | Acinetobacter baylyi |
4.2.1.40 | 38000 | - |
determined by SDS-PAGE | Acinetobacter baylyi |
4.2.1.41 | 51360 | - |
calculated | Acinetobacter baylyi |
4.2.1.41 | 55000 | - |
determined by SDS-PAGE | Acinetobacter baylyi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.40 | D-glucarate | Acinetobacter baylyi | - |
5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | Acinetobacter baylyi | - |
2,5-dioxopentanoate + H2O + CO2 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.2.1.26 | Acinetobacter baylyi | Q6FFQ0 | - |
- |
1.2.1.26 | Pseudomonas putida | Q88GW5 | - |
- |
4.2.1.40 | Acinetobacter baylyi | Q6FFQ2 | - |
- |
4.2.1.41 | Acinetobacter baylyi | Q6FFQ1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.2.1.26 | by gel filtration | Acinetobacter baylyi |
1.2.1.26 | to homogeneity | Pseudomonas putida |
4.2.1.40 | using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column | Acinetobacter baylyi |
4.2.1.41 | using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column | Acinetobacter baylyi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.2.1.26 | 2,5-dioxopentanoate + NAD+ + H2O | - |
Acinetobacter baylyi | 2-oxoglutarate + NADH + H+ | - |
r | |
1.2.1.26 | acetaldehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | acetate + NAD(P)H + H+ | - |
? | |
1.2.1.26 | alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | alpha-ketoglutarate + NAD(P)H + H+ | - |
? | |
1.2.1.26 | alpha-ketoglutaric semialdehyde + NADP+ + H2O | - |
Pseudomonas putida | alpha-ketoglutarate + NADPH + H+ | - |
? | |
1.2.1.26 | glutaraldehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | glutarate + NAD(P)H + H+ | - |
? | |
1.2.1.26 | propanal + NAD(P)+ + H2O | - |
Acinetobacter baylyi | propionate + NAD(P)H + H+ | - |
? | |
1.2.1.26 | succinate semialdehyde + NAD(P)+ + H2O | - |
Acinetobacter baylyi | ? + NAD(P)H + H+ | - |
? | |
4.2.1.40 | D-glucarate | - |
Acinetobacter baylyi | 5-dehydro-4-deoxy-D-glucarate + H2O | - |
? | |
4.2.1.41 | 5-dehydro-4-deoxy-D-glucarate | - |
Acinetobacter baylyi | 2,5-dioxopentanoate + H2O + CO2 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.2.1.26 | homodimer | 2 * 62000, SDS-PAGE | Acinetobacter baylyi |
4.2.1.40 | homotetramer | - |
Acinetobacter baylyi |
4.2.1.41 | homotetramer | - |
Acinetobacter baylyi |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.2.1.26 | alpha-ketoglutarate semialdehyde dehydrogenase | - |
Acinetobacter baylyi |
1.2.1.26 | alpha-ketoglutarate semialdehyde dehydrogenase | - |
Pseudomonas putida |
1.2.1.26 | alpha-KGSA dehydrogenase | - |
Acinetobacter baylyi |
1.2.1.26 | alpha-KGSA dehydrogenase | - |
Pseudomonas putida |
1.2.1.26 | alphaKGSADH | - |
Acinetobacter baylyi |
1.2.1.26 | alphaKGSADH | - |
Pseudomonas putida |
4.2.1.40 | D-Glucarate dehydratase | - |
Acinetobacter baylyi |
4.2.1.40 | GDH | - |
Acinetobacter baylyi |
4.2.1.40 | glucarate dehydratase | - |
Acinetobacter baylyi |
4.2.1.41 | 5-dehydro-4-deoxyglucarate dehydratase | - |
Acinetobacter baylyi |
4.2.1.41 | D-5-keto-4-deoxyglucarate dehydratase | - |
Acinetobacter baylyi |
4.2.1.41 | KDG dehydratase | - |
Acinetobacter baylyi |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.2.1.40 | 22 | - |
activity assay | Acinetobacter baylyi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.2.1.26 | 0.05 | - |
succinate semialdehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.06 | - |
succinate semialdehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.41 | - |
acetaldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.58 | - |
propanal | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 0.65 | - |
Glutaraldehyde | with NADP+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 2.07 | - |
propanal | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 4.13 | - |
Glutaraldehyde | with NAD+ as cofactor | Acinetobacter baylyi | |
1.2.1.26 | 55 | - |
NADP+ | - |
Acinetobacter baylyi | |
1.2.1.26 | 57 | - |
NAD+ | - |
Acinetobacter baylyi | |
1.2.1.26 | 197 | - |
alpha-Ketoglutaric semialdehyde | - |
Pseudomonas putida | |
4.2.1.40 | 6.39 | - |
D-glucarate | - |
Acinetobacter baylyi | |
4.2.1.41 | 3.9 | - |
5-dehydro-4-deoxy-D-glucarate | - |
Acinetobacter baylyi |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.2.1.26 | NAD+ | - |
Acinetobacter baylyi | |
1.2.1.26 | NADP+ | - |
Pseudomonas putida | |
1.2.1.26 | NADP+ | alpha-KGSA dehydrogenase is an NADP+-preferring enzyme, since it is 58times more efficient with NADP+ than with NAD+ | Acinetobacter baylyi |