Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Aghaie, A.; Lechaplais, C.; Sirven, P.; Tricot, S.; Besnard-Gonnet, M.; Muselet, D.; de Berardinis, V.; Kreimeyer, A.; Gyapay, G.; Salanoubat, M.; Perret, A.
    New Insights into the Alternative D-Glucarate Degradation Pathway (2008), J. Biol. Chem., 283, 15638-15646.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.2.1.26 degradation involved in an alternative pathway of D-glucarate metabolism Acinetobacter baylyi
1.2.1.26 degradation involved in an alternative pathway of D-glucarate metabolism Pseudomonas putida

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.26 expression in Escherichia coli Pseudomonas putida
1.2.1.26 expression in Escherichia coli BL21 (DE3) Acinetobacter baylyi
4.2.1.40 into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells Acinetobacter baylyi
4.2.1.41 into the vector pEXP5-NT/TOPO for expression in Escherichia coli BL21DE3 cells Acinetobacter baylyi

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.26 additional information alpha-KGSA dehydrogenase activity in cell extracts from the mutant DELTAACIAD0131 grown on succinate as the sole carbon source is undetectable Acinetobacter baylyi

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.26 additional information no inhibitory effect with alpha-ketoglutarate or glutamate Acinetobacter baylyi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.26 0.014
-
alpha-Ketoglutaric semialdehyde
-
Pseudomonas putida
1.2.1.26 0.015
-
NADP+
-
Acinetobacter baylyi
1.2.1.26 0.1
-
Glutaraldehyde with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 0.3
-
propanal with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 0.31
-
Glutaraldehyde with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 0.45
-
succinate semialdehyde with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 0.87
-
NAD+
-
Acinetobacter baylyi
1.2.1.26 1.05
-
propanal with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 1.66
-
succinate semialdehyde with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 3.9
-
acetaldehyde with NADP+ as cofactor Acinetobacter baylyi
4.2.1.40 0.101
-
D-glucarate
-
Acinetobacter baylyi
4.2.1.41 0.203
-
5-dehydro-4-deoxy-D-glucarate
-
Acinetobacter baylyi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.26 62000
-
2 * 62000, SDS-PAGE Acinetobacter baylyi
4.2.1.40 34960
-
calculated Acinetobacter baylyi
4.2.1.40 38000
-
determined by SDS-PAGE Acinetobacter baylyi
4.2.1.41 51360
-
calculated Acinetobacter baylyi
4.2.1.41 55000
-
determined by SDS-PAGE Acinetobacter baylyi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.40 D-glucarate Acinetobacter baylyi
-
5-dehydro-4-deoxy-D-glucarate + H2O
-
?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate Acinetobacter baylyi
-
2,5-dioxopentanoate + H2O + CO2
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.26 Acinetobacter baylyi Q6FFQ0
-
-
1.2.1.26 Pseudomonas putida Q88GW5
-
-
4.2.1.40 Acinetobacter baylyi Q6FFQ2
-
-
4.2.1.41 Acinetobacter baylyi Q6FFQ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.26 by gel filtration Acinetobacter baylyi
1.2.1.26 to homogeneity Pseudomonas putida
4.2.1.40 using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column Acinetobacter baylyi
4.2.1.41 using a HisTrap FF and a Mono Q column, finally the protein is submitted to gel filtration on a Superdex 200 column Acinetobacter baylyi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.26 2,5-dioxopentanoate + NAD+ + H2O
-
Acinetobacter baylyi 2-oxoglutarate + NADH + H+
-
r
1.2.1.26 acetaldehyde + NAD(P)+ + H2O
-
Acinetobacter baylyi acetate + NAD(P)H + H+
-
?
1.2.1.26 alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
Acinetobacter baylyi alpha-ketoglutarate + NAD(P)H + H+
-
?
1.2.1.26 alpha-ketoglutaric semialdehyde + NADP+ + H2O
-
Pseudomonas putida alpha-ketoglutarate + NADPH + H+
-
?
1.2.1.26 glutaraldehyde + NAD(P)+ + H2O
-
Acinetobacter baylyi glutarate + NAD(P)H + H+
-
?
1.2.1.26 propanal + NAD(P)+ + H2O
-
Acinetobacter baylyi propionate + NAD(P)H + H+
-
?
1.2.1.26 succinate semialdehyde + NAD(P)+ + H2O
-
Acinetobacter baylyi ? + NAD(P)H + H+
-
?
4.2.1.40 D-glucarate
-
Acinetobacter baylyi 5-dehydro-4-deoxy-D-glucarate + H2O
-
?
4.2.1.41 5-dehydro-4-deoxy-D-glucarate
-
Acinetobacter baylyi 2,5-dioxopentanoate + H2O + CO2
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.26 homodimer 2 * 62000, SDS-PAGE Acinetobacter baylyi
4.2.1.40 homotetramer
-
Acinetobacter baylyi
4.2.1.41 homotetramer
-
Acinetobacter baylyi

Synonyms

EC Number Synonyms Comment Organism
1.2.1.26 alpha-ketoglutarate semialdehyde dehydrogenase
-
Acinetobacter baylyi
1.2.1.26 alpha-ketoglutarate semialdehyde dehydrogenase
-
Pseudomonas putida
1.2.1.26 alpha-KGSA dehydrogenase
-
Acinetobacter baylyi
1.2.1.26 alpha-KGSA dehydrogenase
-
Pseudomonas putida
1.2.1.26 alphaKGSADH
-
Acinetobacter baylyi
1.2.1.26 alphaKGSADH
-
Pseudomonas putida
4.2.1.40 D-Glucarate dehydratase
-
Acinetobacter baylyi
4.2.1.40 GDH
-
Acinetobacter baylyi
4.2.1.40 glucarate dehydratase
-
Acinetobacter baylyi
4.2.1.41 5-dehydro-4-deoxyglucarate dehydratase
-
Acinetobacter baylyi
4.2.1.41 D-5-keto-4-deoxyglucarate dehydratase
-
Acinetobacter baylyi
4.2.1.41 KDG dehydratase
-
Acinetobacter baylyi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.40 22
-
activity assay Acinetobacter baylyi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.26 0.05
-
succinate semialdehyde with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 0.06
-
succinate semialdehyde with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 0.41
-
acetaldehyde with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 0.58
-
propanal with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 0.65
-
Glutaraldehyde with NADP+ as cofactor Acinetobacter baylyi
1.2.1.26 2.07
-
propanal with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 4.13
-
Glutaraldehyde with NAD+ as cofactor Acinetobacter baylyi
1.2.1.26 55
-
NADP+
-
Acinetobacter baylyi
1.2.1.26 57
-
NAD+
-
Acinetobacter baylyi
1.2.1.26 197
-
alpha-Ketoglutaric semialdehyde
-
Pseudomonas putida
4.2.1.40 6.39
-
D-glucarate
-
Acinetobacter baylyi
4.2.1.41 3.9
-
5-dehydro-4-deoxy-D-glucarate
-
Acinetobacter baylyi

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.26 NAD+
-
Acinetobacter baylyi
1.2.1.26 NADP+
-
Pseudomonas putida
1.2.1.26 NADP+ alpha-KGSA dehydrogenase is an NADP+-preferring enzyme, since it is 58times more efficient with NADP+ than with NAD+ Acinetobacter baylyi