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Literature summary extracted from

  • Klutts, J.S.; Doering, T.L.
    Cryptococcal xylosyltransferase 1 (Cxt1p) from Cryptococcus neoformans plays a direct role in the synthesis of capsule polysaccharides (2008), J. Biol. Chem., 283, 14327-14334.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.2.38 medicine xylose residues within the capsule polysaccharide are important for normal host-pathogen interaction, loss of CXT1p attenuates virulence Cryptococcus neoformans
2.4.2.38 medicine loss of XT1 leads to attenuation of virulence Cryptococcus neoformans

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.38 5-kb PCR product cloned into the TOPO pCR2.1 vector and maintained in Escherichia coli DH5alpha Cryptococcus neoformans
2.4.2.38 amplification of the gene with flanking regions (1.3 kb upstream of the start codon, 1 kb downstream of the gene) from serotype D (strain JEC21) genomic DNA for cloning in pCR2.1TOPO, generation of replacement cassettes for replacing endogenous CXT1p: (i) nourseothricin acetyltransferase resistance gene flanked by the CXT1p flanking regions, transformed as linear DNA in CAP67 strain, (ii) cxt1 gene lacking 3’ two-thirds of the encoding sequence flanked by its flanking regions, transformed as linear DNA in JEC21 strain Cryptococcus neoformans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.38 UDP-D-xylose + galactoxylomannan Cryptococcus neoformans
-
 UDP + [beta-D-xylose-(1,2)]-galactoxylomannan JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: almost complete loss of beta-xylose residues (1,2)-linked to mannose, unchanged amounts of beta-xylose (1,3)-linked to galactose ?
2.4.2.38 UDP-D-xylose + glucuronoxylomannan Cryptococcus neoformans
-
 UDP + [beta-D-xylose-(1,2)]-glucuronoxylomannan JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: 30% decrease in amount of xylose, 39% decrease in O-2 xylose-substituted mannose, 31.5% increase in O-2-unsubstituted mannose, loss of 1/3 of beta-(1,2)-linked xylose, revealed by NMR analyses ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.38 Cryptococcus neoformans
-
 wild-type strain JEC20 (serotype D MAT a), wild-type strain JEC21 (serotype D MAT alpha), mutant strain CAP67 (serotype D MAT alpha cap59), mutant strain JEC21/cxt1delta (serotype D, isogenic MAT alpha, encapsulated and nourseothricin-resistant, lacking CXT1p), mutant strain CAP67/cxt1delta (serotype D, isogenic MAT alpha, acapsulated and nourseothricin-resistant, lacking CXT1p)
-
2.4.2.38 Cryptococcus neoformans Q5K8R6 strains JEC20, JEC21 and CAP67
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.38 additional information lack of CXT1p in membranes from JEC21/cxt1delta and CAP67/cxt1delta leads to dramatic reduction in D-xylose-beta-(1,2)-D-mannose-alpha-(1,3)-D-mannose synthesising activity in presence of exogenously added substrate Cryptococcus neoformans ?
-
 ?
2.4.2.38 UDP-D-xylose + D-mannosyl-alpha-(1,3)-D-mannose membranes, radiolabelled UDP-xylose, alpha-(1,3)-mannobiose (resembles backbone or dimannose moiety of natural substrates) Cryptococcus neoformans UDP + D-xylosyl-beta-(1,2)-D-mannosyl-alpha-(1,3)-D-mannose product: trisaccharide with xylose moiety linked to reducing mannose of alpha-(1,3)-mannobiose, radiolabel detection by scintillation counting or thin layer chromatography and autoradiography, product purification for NMR and glycosyl linkage analyses ?
2.4.2.38 UDP-D-xylose + galactoxylomannan
-
 Cryptococcus neoformans UDP + [beta-D-xylose-(1,2)]-galactoxylomannan JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: almost complete loss of beta-xylose residues (1,2)-linked to mannose, unchanged amounts of beta-xylose (1,3)-linked to galactose ?
2.4.2.38 UDP-D-xylose + glucuronoxylomannan
-
 Cryptococcus neoformans UDP + [beta-D-xylose-(1,2)]-glucuronoxylomannan JEC21/cxt1delta and CAP67/cxt1delta lacking CXT1p: 30% decrease in amount of xylose, 39% decrease in O-2 xylose-substituted mannose, 31.5% increase in O-2-unsubstituted mannose, loss of 1/3 of beta-(1,2)-linked xylose, revealed by NMR analyses ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.38 beta-1,2-xylosyltransferase
-
 Cryptococcus neoformans
2.4.2.38 beta1,2-xylosyltransferase
-
 Cryptococcus neoformans
2.4.2.38 Cryptococcal xylosyltransferase
-
 Cryptococcus neoformans
2.4.2.38 Cxt1p
-
 Cryptococcus neoformans
2.4.2.38 XT1
-
 Cryptococcus neoformans
2.4.2.38 Xt1p
-
 Cryptococcus neoformans
2.4.2.38 xylosyltransferase I
-
 Cryptococcus neoformans

General Information

EC Number General Information Comment Organism
2.4.2.38 malfunction loss of XT1 does not affect in vitro growth of mutant cells or general morphology of their capsules. The two main capsule polysaccharides, glucuronoxylomannan and galactoxylomannan, both are missing beta1,2-xylose residues. Deletion of XT1 leads to attenuation of cryptococcal growth in a mouse model of infection Cryptococcus neoformans
2.4.2.38 physiological function Xt1p has a defined role in capsule biosynthesis Cryptococcus neoformans