Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Watanabe, S.; Yamada, M.; Ohtsu, I.; Makino, K.
    alpha-ketoglutaric semialdehyde dehydrogenase isozymes involved in metabolic pathways of D-glucarate, D-galactarate, and hydroxy-L-proline. Molecular and metabolic convergent evolution (2007), J. Biol. Chem., 282, 6685-6695.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.26 D-galactarate induces KGSADH-II Azospirillum brasilense
1.2.1.26 D-glucarate induces KGSADH-II Azospirillum brasilense
1.2.1.26 hydroxy-L-proline induces KGSADH-III Azospirillum brasilense
1.2.1.26 L-arabinose induces KGSADH-I Azospirillum brasilense

Application

EC Number Application Comment Organism
1.2.1.26 additional information the ALDH branch including ycbD protein is designated the KGSADH subclass (type III) Bacillus subtilis
1.2.1.26 additional information three different types of KGSADH appear in the bacterial evolutional stage convergently Azospirillum brasilense

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.26 expressed as His6-tagged ycbD protein Bacillus subtilis
1.2.1.26 KGSADH-II overexpressed as His6-tagged protein in Escherichia coli DH5alpha Azospirillum brasilense
1.2.1.26 KGSADH-III overexpressed as His6-tagged protein in Escherichia coli DH5alpha Azospirillum brasilense

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.26 0.0065
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
1.2.1.26 0.0075
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
1.2.1.26 0.011
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
1.2.1.26 0.0149
-
 NADP+
-
 Azospirillum brasilense
1.2.1.26 0.0246
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
1.2.1.26 0.0352
-
 Octylaldehyde
-
 Bacillus subtilis
1.2.1.26 0.0393
-
 heptaldehyde
-
 Bacillus subtilis
1.2.1.26 0.0622
-
 NADP+
-
 Azospirillum brasilense
1.2.1.26 0.0814
-
 hexylaldehyde
-
 Bacillus subtilis
1.2.1.26 0.094
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Bacillus subtilis
1.2.1.26 0.1169
-
 NAD+
-
 Azospirillum brasilense
1.2.1.26 0.145
-
 Valeraldehyde
-
 Bacillus subtilis
1.2.1.26 0.17
-
 butylaldehyde
-
 Bacillus subtilis
1.2.1.26 0.2599
-
 NAD+ KGSADH-I Azospirillum brasilense
1.2.1.26 0.5569
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Bacillus subtilis
1.2.1.26 0.74
-
 NAD+
-
 Bacillus subtilis
1.2.1.26 1.08
-
 NADP+
-
 Bacillus subtilis
1.2.1.26 1.136
-
 NAD+
-
 Azospirillum brasilense
1.2.1.26 1.46
-
 propionaldehyde
-
 Bacillus subtilis
1.2.1.26 2.427
-
 NADP+ KGSADH-I Azospirillum brasilense

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.26 12000
-
 gel filtration Azospirillum brasilense
1.2.1.26 54351
-
 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
1.2.1.26 55185
-
 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense
1.2.1.26 57000
-
 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
1.2.1.26 57000
-
 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.26 Azospirillum brasilense Q08IB7 strain ATCC29145
-
1.2.1.26 Azospirillum brasilense Q08IC0 strain ATCC29145
-
1.2.1.26 Azospirillum brasilense Q1JUP4 strain ATCC29145
-
1.2.1.26 Azospirillum brasilense ATCC 29145 Q08IB7 strain ATCC29145
-
1.2.1.26 Azospirillum brasilense ATCC 29145 Q08IC0 strain ATCC29145
-
1.2.1.26 Azospirillum brasilense ATCC 29145 Q1JUP4 strain ATCC29145
-
1.2.1.26 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.26 KGSADH-II purified on nickel-nitrilotriacetic acid spin column and by gel filtration Azospirillum brasilense
1.2.1.26 KGSADH-III purified on nickel-nitrilotriacetic acid spin column and by gel filtration Azospirillum brasilense

Storage Stability

EC Number Storage Stability Organism
1.2.1.26 -35°C, buffer E, pH 8.0, 250 mM imidazole Azospirillum brasilense

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.26 2,5-dioxopentanoate + NAD+ + H2O
-
 Bacillus subtilis 2-oxoglutarate + NADH + H+
-
 r
1.2.1.26 2,5-dioxopentanoate + NAD+ + H2O
-
 Azospirillum brasilense 2-oxoglutarate + NADH + H+
-
 r
1.2.1.26 2,5-dioxopentanoate + NAD+ + H2O
-
 Azospirillum brasilense ATCC 29145 2-oxoglutarate + NADH + H+
-
 r
1.2.1.26 alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Bacillus subtilis alpha-ketoglutarate + NAD(P)H + H+
-
 ?
1.2.1.26 alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Azospirillum brasilense alpha-ketoglutarate + NAD(P)H + H+
-
 ?
1.2.1.26 alpha-ketoglutaric semialdehyde + NAD(P)+ + H2O
-
 Azospirillum brasilense ATCC 29145 alpha-ketoglutarate + NAD(P)H + H+
-
 ?
1.2.1.26 butyraldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-butanoate + NAD(P)H + H+
-
 ?
1.2.1.26 heptaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-heptanoate + NAD(P)H + H+
-
 ?
1.2.1.26 hexylaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-hexanoate + NAD(P)H + H+
-
 ?
1.2.1.26 octylaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-octanoate + NAD(P)H + H+
-
 ?
1.2.1.26 propionaldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis propionate + NAD(P)H + H+
-
 ?
1.2.1.26 valeraldehyde + NAD(P)+ + H2O
-
 Bacillus subtilis n-pentanoate + NAD(P)H + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.26 dimer 2 * 57000 or 2 * 54351, gel filtration Azospirillum brasilense
1.2.1.26 dimer 2 * 57000 or 2 * 55185, gel filtration Azospirillum brasilense
1.2.1.26 tetramer gel filtration Bacillus subtilis
1.2.1.26 tetramer gel filtration Azospirillum brasilense

Synonyms

EC Number Synonyms Comment Organism
1.2.1.26 alpha-ketoglutaric semialdehyde dehydrogenase
-
 Bacillus subtilis
1.2.1.26 alpha-ketoglutaric semialdehyde dehydrogenase
-
 Azospirillum brasilense
1.2.1.26 alphaKGSA dehydrogenase
-
 Bacillus subtilis
1.2.1.26 alphaKGSA dehydrogenase
-
 Azospirillum brasilense
1.2.1.26 KGSADH
-
 Bacillus subtilis
1.2.1.26 KGSADH-I
-
 Azospirillum brasilense
1.2.1.26 KGSADH-II
-
 Azospirillum brasilense
1.2.1.26 KGSADH-III
-
 Azospirillum brasilense
1.2.1.26 ycbD protein
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.26 0.74
-
 NAD+
-
 Bacillus subtilis
1.2.1.26 0.87
-
 heptaldehyde
-
 Bacillus subtilis
1.2.1.26 0.97
-
 Octylaldehyde
-
 Bacillus subtilis
1.2.1.26 1.08
-
 NADP+
-
 Bacillus subtilis
1.2.1.26 1.12
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Bacillus subtilis
1.2.1.26 1.29
-
 propionaldehyde
-
 Bacillus subtilis
1.2.1.26 1.35
-
 butylaldehyde
-
 Bacillus subtilis
1.2.1.26 1.47
-
 hexylaldehyde
-
 Bacillus subtilis
1.2.1.26 2.1
-
 Valeraldehyde
-
 Bacillus subtilis
1.2.1.26 2.75
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Bacillus subtilis
1.2.1.26 9.86
-
 NADP+ KGSADH-I Azospirillum brasilense
1.2.1.26 10.2
-
 NADP+
-
 Azospirillum brasilense
1.2.1.26 15.3
-
 NADP+
-
 Azospirillum brasilense
1.2.1.26 15.4
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
1.2.1.26 16.7
-
 NAD+ KGSADH-I Azospirillum brasilense
1.2.1.26 21.3
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense
1.2.1.26 23
-
 alpha-Ketoglutaric semialdehyde with NADP+ as cofactor Azospirillum brasilense
1.2.1.26 35.5
-
 NAD+
-
 Azospirillum brasilense
1.2.1.26 52.3
-
 NAD+
-
 Azospirillum brasilense
1.2.1.26 53.9
-
 alpha-Ketoglutaric semialdehyde with NAD+ as cofactor Azospirillum brasilense

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.26 NAD+
-
 Bacillus subtilis
1.2.1.26 NAD+
-
 Azospirillum brasilense
1.2.1.26 NAD+ no significant preference between NAD+ and NADP+ in activity Azospirillum brasilense
1.2.1.26 NAD+ prefered cofactor of KGSADH-II Azospirillum brasilense
1.2.1.26 NADP+
-
 Bacillus subtilis
1.2.1.26 NADP+
-
 Azospirillum brasilense
1.2.1.26 NADP+ prefered cofactor of KGSADH-III Azospirillum brasilense