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Literature summary extracted from

  • Naula, C.; Alibu, V.P.; Brock, J.M.; Veitch, N.J.; Burchmore, R.J.; Barrett, M.P.
    A new erythrose 4-phosphate dehydrogenase coupled assay for transketolase (2008), J. Biochem. Biophys. Methods, 70, 1185-1187.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.2.1.72 degradation coupling of a transketolase reaction (using Leishmania mexicana transketolase) that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using E4PD, whereby D-ribose 5-phosphate and D-glyceraldehyde 3-phosphate can both be used as ketol acceptor substrates in the reaction Escherichia coli
2.2.1.1 analysis coupling of a transketolase reaction that converts D-fructose 6-phosphate to D-erythrose 4-phosphate, which can then be converted to 4-phosphate D-erythronate using erythrose-4-phosphate dehydrogenase, a reaction that reduces NAD+ to NADH and can be easily followed spectrophotometrically Leishmania mexicana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.1
-
Leishmania mexicana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.2.1.1 fructose 6-phosphate at concentrations higher than 10 mM fructose 6-phosphate causes an inhibition of the enzyme Leishmania mexicana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.1 0.61
-
fructose 6-phosphate
-
Leishmania mexicana

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.1 Mg2+
-
Leishmania mexicana

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.72 Escherichia coli P0A9B6
-
-
2.2.1.1 Leishmania mexicana Q8MPM3
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.2.1.1 1.8
-
using 10 mM D-glyceraldehyde 3-phosphate Leishmania mexicana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.72 D-erythrose 4-phosphate + NAD+ production of E4P can be coupled to the NADH generating E4PD activity which enables measurement of the transketolase reaction involving F6P as donor substrate Escherichia coli 4-phosphoerythronate + NADH
-
?
1.2.1.72 D-ribose 5-phosphate + NAD+ D-Ribose 5-phosphate can be used as ketol acceptor substrate in the reaction although D-ribose 5-phosphate is also a substrate for the coupling enzyme Escherichia coli ? + NADH
-
?
1.2.1.72 additional information fructose 6-phosphate, glycerol 6-phosphate and glyceraldehyde 3-phosphate can not be used as substrate by E4PD when added up to 50 mM Escherichia coli ?
-
?
2.2.1.1 D-fructose 6-phosphate + D-glyceraldehyde 3-phosphate
-
Leishmania mexicana D-erythrose 4-phosphate + D-xylulose 5-phosphate
-
?
2.2.1.1 D-xylulose 5-phosphate + D-ribose 5-phosphate
-
Leishmania mexicana sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
1.2.1.72 E4PD
-
Escherichia coli
2.2.1.1 TKT
-
Leishmania mexicana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.72 NAD+
-
Escherichia coli
2.2.1.1 thiamine diphosphate
-
Leishmania mexicana