Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Suzuki, K.; Ito, S.; Shimizu-Ibuka, A.; Sakai, H.
    Crystal structure of pyruvate kinase from Geobacillus stearothermophilus (2008), J. Biochem., 144, 305-312.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.40 AMP wild-type, A0.5 value 0.013 mM Geobacillus stearothermophilus
2.7.1.40 D-ribose 5-phosphate wild-type, A0.5 value 0.0075 mM Geobacillus stearothermophilus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.40 expression in Escherichia coli Geobacillus stearothermophilus

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.1.40 mutant C9S/C268S, 2.4 A resolution. Crystal belongs to space group P6222. Enzyme is a homotetramer, a sulfate ion is bound in a pocket in the effector-binding C domain Geobacillus stearothermophilus

Protein Variants

EC Number Protein Variants Comment Organism
2.7.1.40 C9S/C268S crystallization data Geobacillus stearothermophilus
2.7.1.40 H425A the A0.5 values for H425A are increased 8.0- and 60fold for D-ribose 5-phosphate and AMP Geobacillus stearothermophilus
2.7.1.40 V435E about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical Geobacillus stearothermophilus
2.7.1.40 V435K about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical Geobacillus stearothermophilus
2.7.1.40 V435R about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical Geobacillus stearothermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.40 Geobacillus stearothermophilus Q02499
-
-

Subunits

EC Number Subunits Comment Organism
2.7.1.40 tetramer crystallization data Geobacillus stearothermophilus