Literature summary extracted from
Suzuki, K.; Ito, S.; Shimizu-Ibuka, A.; Sakai, H.
Crystal structure of pyruvate kinase from Geobacillus stearothermophilus (2008), J. Biochem., 144, 305-312.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.7.1.40 |
AMP |
wild-type, A0.5 value 0.013 mM |
Geobacillus stearothermophilus |
|
2.7.1.40 |
D-ribose 5-phosphate |
wild-type, A0.5 value 0.0075 mM |
Geobacillus stearothermophilus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
2.7.1.40 |
expression in Escherichia coli |
Geobacillus stearothermophilus |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
2.7.1.40 |
mutant C9S/C268S, 2.4 A resolution. Crystal belongs to space group P6222. Enzyme is a homotetramer, a sulfate ion is bound in a pocket in the effector-binding C domain |
Geobacillus stearothermophilus |
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
2.7.1.40 |
C9S/C268S |
crystallization data |
Geobacillus stearothermophilus |
2.7.1.40 |
H425A |
the A0.5 values for H425A are increased 8.0- and 60fold for D-ribose 5-phosphate and AMP |
Geobacillus stearothermophilus |
2.7.1.40 |
V435E |
about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical |
Geobacillus stearothermophilus |
2.7.1.40 |
V435K |
about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical |
Geobacillus stearothermophilus |
2.7.1.40 |
V435R |
about fourfold increase in A0.5 values for D-ribose 5-phosphate compared with that of the wild-type enzyme. The A0.5 values for AMP and the S0.5 values are essentially identical |
Geobacillus stearothermophilus |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.7.1.40 |
Geobacillus stearothermophilus |
Q02499 |
- |
- |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.7.1.40 |
tetramer |
crystallization data |
Geobacillus stearothermophilus |