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Literature summary extracted from

  • Kandeel, M.; Kitade, Y.
    Molecular characterization, heterologous expression and kinetic analysis of recombinant Plasmodium falciparum thymidylate kinase (2008), J. Biochem., 144, 245-250.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.9 expression in Escherichia coli Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.4.9 dAMP 5 mM, about 15% inhibition Plasmodium falciparum
2.7.4.9 dUMP 5 mM, about 15% inhibition Plasmodium falciparum
2.7.4.9 EDTA 4 mM, 80% loss of activity Plasmodium falciparum
2.7.4.9 additional information not inhibitory: 2-mercaptoethanol up to 5 mM, AMP, CMP, dCMP, GMP and dGMP and Plasmodium falciparum
2.7.4.9 UMP 5 mM, about 15% inhibition Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.9 0.022
-
 dTMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 0.03
-
 dGMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 0.577
-
 dIMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 0.78
-
 dUMP pH 7.2, 30°C Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.9 Plasmodium falciparum
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.9 2.2
-
 substrate dIMP, pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 6.5
-
 substrate dUMP, pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 13
-
 substrate dGMP, pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 15
-
 substrate dTMP, pH 7.2, 30°C Plasmodium falciparum

Storage Stability

EC Number Storage Stability Organism
2.7.4.9 -20°C, 2 months, no loss of activity Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.9 ATP + dGMP 86% of the activity with dTMP Plasmodium falciparum ADP + dGDP
-
 ?
2.7.4.9 ATP + dIMP 15% of the activity with dTMP Plasmodium falciparum ADP + dIDP
-
 ?
2.7.4.9 ATP + dTMP
-
 Plasmodium falciparum ADP + dTDP
-
 ?
2.7.4.9 ATP + dUMP 43% of the activity with dTMP Plasmodium falciparum ADP + dUDP
-
 ?
2.7.4.9 ATP + GMP 1.5% of the activity with dTMP Plasmodium falciparum ADP + GDP
-
 ?
2.7.4.9 dATP + dTMP dATP, 64% of the activity with ATP Plasmodium falciparum dADP + dTDP
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.4.9 60
-
 10 min, 30% residual activity Plasmodium falciparum
2.7.4.9 65
-
 10 min, complete loss of activity Plasmodium falciparum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.4.9 0.44
-
 dIMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 2.9
-
 dGMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 3.4
-
 dTMP pH 7.2, 30°C Plasmodium falciparum
2.7.4.9 4.1
-
 dUMP pH 7.2, 30°C Plasmodium falciparum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.4.9 6
-
 marked drop in activity below Plasmodium falciparum
2.7.4.9 7 9
-
 Plasmodium falciparum