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Literature summary extracted from

  • Bratkovic, T.; Lunder, M.; Urleb, U.; Strukelj, B.
    Peptide inhibitors of MurD and MurE, essential enzymes of bacterial cell wall biosynthesis (2008), J. Basic Microbiol., 48, 202-206.
    View publication on PubMed

Application

EC Number Application Comment Organism
6.3.2.9 drug development MurD is a target for small molecule chemotherapeutics Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.2.9 CPAHWPHPC i.e. peptide MD-C7C_1, inhibits MurD Escherichia coli
6.3.2.9 CSAWSNKFC i.e. peptide MD-C7C_2, inhibits MurD Escherichia coli
6.3.2.9 HSSWYIQHFPPL i.e. peptide MD-12, inhibits MurD Escherichia coli
6.3.2.9 additional information design of peptidomimetic lead compounds with the ultimate objective of small molecule chemotherapeutic development, affinity selection by immobilized MurD with addition of ATP in an attempt to achieve conformationally homogenous population of target enzymes, overview. No inhibition by peptide MD-C7C_3, i.e. CQSSPHMSC Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
6.3.2.9 cytoplasm
-
 Escherichia coli 5737
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.2.9 Mg2+
-
 Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.2.9 ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate Escherichia coli MurD is essential for cytoplasmic bacterial cell wall biosynthesis ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.2.9 Escherichia coli
-
 gene murD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.2.9 ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate
-
 Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 ?
6.3.2.9 ATP + UDP-N-acetylmuramyl-L-alanine + D-glutamate MurD is essential for cytoplasmic bacterial cell wall biosynthesis Escherichia coli ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.2.9 More all Mur ligases are topologically similar to each other, extremely flexible, and built of three domains connected by two hinges. Each domain binds one of the three substrates, i.e. ATP, growing nucleotide, or the appropriate amino acid Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
6.3.2.9 MurD
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.2.9 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.2.9 8
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.2.9 ATP
-
 Escherichia coli

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.2.9 0.14
-
 pH 8.0, 37°C Escherichia coli HSSWYIQHFPPL
6.3.2.9 0.62
-
 pH 8.0, 37°C Escherichia coli CSAWSNKFC
6.3.2.9 1.5
-
 pH 8.0, 37°C Escherichia coli CPAHWPHPC