EC Number | Application | Comment | Organism |
---|---|---|---|
1.8.1.4 | additional information | a mutation in LPD leads to a pyruvate dehydrogenase complex that is less sensitive to inhibition by NADH, allowing the enzyme to function in an anaerobic culture, which changes the fermentation profile of the mutant. Presence and functional activity of such an NADH-insensitive pyruvate dehydrogenase may have significant unexplored physiological and biotechnological applications | Escherichia coli K-12 |
EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.4 | expressed in Escherichia coli M109(lambdaDE3) | Escherichia coli K-12 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.4 | E354K | is significantly less sensitive to NADH inhibition than the native LPD | Escherichia coli K-12 |
1.8.1.4 | H322Y | upon purification LPD loses activity and associated FAD at the gel filtration step | Escherichia coli K-12 |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | FAD | - |
Escherichia coli K-12 | |
1.8.1.4 | NADH | - |
Escherichia coli K-12 |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 0.07 | - |
NAD+ | native LPD | Escherichia coli K-12 | |
1.8.1.4 | 0.08 | - |
NAD+ | mutant E354K | Escherichia coli K-12 | |
1.8.1.4 | 0.11 | - |
NAD+ | mutant H322Y | Escherichia coli K-12 | |
1.8.1.4 | 0.3 | - |
pyruvate | mutant E354K | Escherichia coli K-12 | |
1.8.1.4 | 0.4 | - |
pyruvate | native LPD | Escherichia coli K-12 | |
1.8.1.4 | 0.48 | - |
pyruvate | mutant H322Y | Escherichia coli K-12 |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.4 | Escherichia coli K-12 | - |
strains AH242 SE2378 and SE2382 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.4 | gel filtration | Escherichia coli K-12 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | lipoamide + NADH | - |
Escherichia coli K-12 | dihydrolipoamide + NAD+ | - |
r | |
1.8.1.4 | pyruvate + NADH | - |
Escherichia coli K-12 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.4 | dihydrolipoamide dehydrogenase | - |
Escherichia coli K-12 |
1.8.1.4 | LPD | - |
Escherichia coli K-12 |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | NAD+ | - |
Escherichia coli K-12 | |
1.8.1.4 | NADH | - |
Escherichia coli K-12 |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 0.001 | - |
NADH | native pyruvate dehydrogenase complex | Escherichia coli K-12 | |
1.8.1.4 | 0.0096 | - |
NADH | mutant H322Y | Escherichia coli K-12 | |
1.8.1.4 | 0.01 | - |
NADH | mutant E354K | Escherichia coli K-12 |