Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Kim, Y.; Ingram, L.O.; Shanmugam, K.T.
    Dihydrolipoamide dehydrogenase mutation alters the NADH sensitivity of pyruvate dehydrogenase complex of Escherichia coli K-12 (2008), J. Bacteriol., 190, 3851-3858.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.8.1.4 additional information a mutation in LPD leads to a pyruvate dehydrogenase complex that is less sensitive to inhibition by NADH, allowing the enzyme to function in an anaerobic culture, which changes the fermentation profile of the mutant. Presence and functional activity of such an NADH-insensitive pyruvate dehydrogenase may have significant unexplored physiological and biotechnological applications Escherichia coli K-12

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.1.4 expressed in Escherichia coli M109(lambdaDE3) Escherichia coli K-12

Protein Variants

EC Number Protein Variants Comment Organism
1.8.1.4 E354K is significantly less sensitive to NADH inhibition than the native LPD Escherichia coli K-12
1.8.1.4 H322Y upon purification LPD loses activity and associated FAD at the gel filtration step Escherichia coli K-12

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 FAD
-
Escherichia coli K-12
1.8.1.4 NADH
-
Escherichia coli K-12

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.07
-
NAD+ native LPD Escherichia coli K-12
1.8.1.4 0.08
-
NAD+ mutant E354K Escherichia coli K-12
1.8.1.4 0.11
-
NAD+ mutant H322Y Escherichia coli K-12
1.8.1.4 0.3
-
pyruvate mutant E354K Escherichia coli K-12
1.8.1.4 0.4
-
pyruvate native LPD Escherichia coli K-12
1.8.1.4 0.48
-
pyruvate mutant H322Y Escherichia coli K-12

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Escherichia coli K-12
-
strains AH242 SE2378 and SE2382
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 gel filtration Escherichia coli K-12

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 lipoamide + NADH
-
Escherichia coli K-12 dihydrolipoamide + NAD+
-
r
1.8.1.4 pyruvate + NADH
-
Escherichia coli K-12 ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
Escherichia coli K-12
1.8.1.4 LPD
-
Escherichia coli K-12

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 NAD+
-
Escherichia coli K-12
1.8.1.4 NADH
-
Escherichia coli K-12

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.8.1.4 0.001
-
NADH native pyruvate dehydrogenase complex Escherichia coli K-12
1.8.1.4 0.0096
-
NADH mutant H322Y Escherichia coli K-12
1.8.1.4 0.01
-
NADH mutant E354K Escherichia coli K-12