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Literature summary extracted from

  • Wierckx, N.J.; Ballerstedt, H.; de Bont, J.A.; de Winde, J.H.; Ruijssenaars, H.J.; Wery, J.
    Transcriptome analysis of a phenol-producing Pseudomonas putida S12 construct: genetic and physiological basis for improved production (2008), J. Bacteriol., 190, 2822-2830.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.99.2 Targeted gene disruptions are performed. Gene replacement vectors for the hpd, pobA, phhA, and aroP1 genes are created from pJQ200SK with different primers. Vectors are used to mutate the selected genes in Pseudomonas putida S12TPL3c by homologous recombination. Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.99.2 phenol In presence of 1 mM, only 23% of the original activity remained. The presence of phenol seriously affects its own production, through the inhibition of TPL Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.99.2 L-tyrosine + H2O Pseudomonas putida
-
 phenol + pyruvate + NH3
-
 r

Organism

EC Number Organism UniProt Comment Textmining
4.1.99.2 Pseudomonas putida
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.1.99.2 cell culture wild-type S12 and its mutant strains S12TPL3 (optimized for phenol production) Pseudomonas putida
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.99.2 0.0022
-
 in strain S12TPL3-delta-hpd mutant (4-hydroxyphenylpyruvate dioxygenase knockout), harvested in logarithmic phase Pseudomonas putida
4.1.99.2 0.0053
-
 in strain S12TPL3, in presence of 1 mM phenol Pseudomonas putida
4.1.99.2 0.0078
-
 in strain S12TPL3, harvested in logarithmic phase Pseudomonas putida
4.1.99.2 0.0232
-
 in strain S12TPL3, without phenol Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.99.2 L-tyrosine + H2O
-
 Pseudomonas putida phenol + pyruvate + NH3
-
 r

Synonyms

EC Number Synonyms Comment Organism
4.1.99.2 TPL
-
 Pseudomonas putida
4.1.99.2 tyrosine-phenol lyase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.1.99.2 30
-
 assay at Pseudomonas putida