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Literature summary extracted from

  • Coleman, J.P.; Hudson, L.L.; McKnight, S.L.; Farrow, J.M.; Calfee, M.W.; Lindsey, C.A.; Pesci, E.C.
    Pseudomonas aeruginosa PqsA is an anthranilate-coenzyme A ligase (2008), J. Bacteriol., 190, 1247-1255.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.2.1.32 gene pqsA is part of the pqsABCDE operon, sequence comparisons, expression of the His-tagged enzyme in Escherichia coli Pseudomonas aeruginosa

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.2.1.32 2-amino-3-chlorobenzoate
-
 Pseudomonas aeruginosa
6.2.1.32 3-fluoro-O-anisidine
-
 Pseudomonas aeruginosa
6.2.1.32 5-nitroanthranilonitrile
-
 Pseudomonas aeruginosa
6.2.1.32 anthranilonitrile
-
 Pseudomonas aeruginosa
6.2.1.32 methylanthranilate i.e. 2-aminobenzoate methyl ester Pseudomonas aeruginosa
6.2.1.32 additional information effects of substrate analogues on in vitro anthranilate-CoA ligase activity and in vivo PQS production in Pseudomonas aeruginosa, overview Pseudomonas aeruginosa
6.2.1.32 salicylate
-
 Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.32 Mg2+
-
 Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.32 ATP + anthranilate + CoA Pseudomonas aeruginosa the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview AMP + diphosphate + anthranilyl-CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.32 Pseudomonas aeruginosa Q9I4X3 gene pqsA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.32 recombinant His-tagged PqsA 58fold from Escherichia coli by ammonium sulfate fractionation, metal affinity and hydrophobic interaction chromatography, and anion exchange chromatography Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.32 ATP + anthranilate + CoA
-
 Pseudomonas aeruginosa AMP + diphosphate + anthranilyl-CoA
-
 ?
6.2.1.32 ATP + anthranilate + CoA the Pseudomonas quinolone signal PQS is a 3-hydroxy-4-quinolone with a 2-alkyl substitution, which is synthesized by the condensation of anthranilic acid with a 3-keto-fatty acid, pathway for PQS biosynthesis, anthranilate is activated by PqsA to form anthraniloyl-CoA, and this is condensed with 2-oxo-decanoyl-ACP which is recruited from the fatty acid biosynthetic pathway, overview Pseudomonas aeruginosa AMP + diphosphate + anthranilyl-CoA
-
 ?

Synonyms

EC Number Synonyms Comment Organism
6.2.1.32 anthranilate-coenzyme A ligase
-
 Pseudomonas aeruginosa
6.2.1.32 PqsA
-
 Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.32 37
-
 assay at Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.32 8.5
-
-
 Pseudomonas aeruginosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
6.2.1.32 7 9
-
 Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
6.2.1.32 ATP
-
 Pseudomonas aeruginosa

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
6.2.1.32 0.0129
-
 2-amino-3-chlorobenzoate pH 8.0, 37°C Pseudomonas aeruginosa
6.2.1.32 0.0183
-
 salicylate pH 8.0, 37°C Pseudomonas aeruginosa
6.2.1.32 0.037
-
 5-nitroanthranilonitrile pH 8.0, 37°C Pseudomonas aeruginosa
6.2.1.32 0.0897
-
 3-fluoro-O-anisidine pH 8.0, 37°C Pseudomonas aeruginosa
6.2.1.32 1.3
-
 anthranilonitrile pH 8.0, 37°C Pseudomonas aeruginosa
6.2.1.32 5.8
-
 methylanthranilate pH 8.0, 37°C Pseudomonas aeruginosa