EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.1.3.26 | 2,3-Butanedione | - |
Thermothelomyces heterothallicus | |
3.1.3.26 | Al3+ | inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Ba2+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Co2+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Cu2+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Fe2+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Fe3+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | guanidinium hydrochloride | - |
Thermothelomyces heterothallicus | |
3.1.3.26 | KI | - |
Thermothelomyces heterothallicus | |
3.1.3.26 | Mg2+ | slight stimulatory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Mn2+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Na+ | slight inhibitory effect | Thermothelomyces heterothallicus | |
3.1.3.26 | Urea | - |
Thermothelomyces heterothallicus | |
3.1.3.26 | Zn2+ | slight inhibitory effect | Thermothelomyces heterothallicus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.1.3.26 | 0.156 | - |
myo-inositol hexakisphosphate | Vmax: 83 nmol/mg/s | Thermothelomyces heterothallicus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | 90000 | - |
SDS-PAGE | Thermothelomyces heterothallicus |
3.1.3.26 | 456000 | - |
homopentamer, native PAGE | Thermothelomyces heterothallicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.1.3.26 | Thermothelomyces heterothallicus | - |
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
3.1.3.26 | glycoprotein | 14% carbohydrate | Thermothelomyces heterothallicus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.1.3.26 | purified to homogeneity using acetone precipitation followed by ion-exchange and gel-filtration column chromatography | Thermothelomyces heterothallicus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.1.3.26 | 4-nitrophenyl phosphate + H2O | - |
Thermothelomyces heterothallicus | 4-nitrophenol + phosphate | - |
? | |
3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Thermothelomyces heterothallicus | ? + phosphate | - |
? | |
3.1.3.26 | phosphoenol pyruvate + H2O | - |
Thermothelomyces heterothallicus | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.1.3.26 | homopentamer | 4 * 90000, SDS-PAGE, gel filtration | Thermothelomyces heterothallicus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.1.3.26 | phytase | - |
Thermothelomyces heterothallicus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | 60 | - |
- |
Thermothelomyces heterothallicus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | additional information | - |
T1/2 of 16 h at 60°C and 1.5 h at 80°C | Thermothelomyces heterothallicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.1.3.26 | 5 | - |
- |
Thermothelomyces heterothallicus |