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Literature summary extracted from

  • Singh, B.; Satyanarayana, T.
    Phytase production by Sporotrichum thermophile in a cost-effective cane molasses medium in submerged fermentation and its application in bread (2008), J. Appl. Microbiol., 105, 1858-1865.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.3.26 2,3-Butanedione
-
Thermothelomyces heterothallicus
3.1.3.26 Al3+ inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Ba2+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Co2+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Cu2+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Fe2+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Fe3+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 guanidinium hydrochloride
-
Thermothelomyces heterothallicus
3.1.3.26 KI
-
Thermothelomyces heterothallicus
3.1.3.26 Mg2+ slight stimulatory effect Thermothelomyces heterothallicus
3.1.3.26 Mn2+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Na+ slight inhibitory effect Thermothelomyces heterothallicus
3.1.3.26 Urea
-
Thermothelomyces heterothallicus
3.1.3.26 Zn2+ slight inhibitory effect Thermothelomyces heterothallicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.26 0.156
-
myo-inositol hexakisphosphate Vmax: 83 nmol/mg/s Thermothelomyces heterothallicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.3.26 90000
-
SDS-PAGE Thermothelomyces heterothallicus
3.1.3.26 456000
-
homopentamer, native PAGE Thermothelomyces heterothallicus

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.26 Thermothelomyces heterothallicus
-
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.3.26 glycoprotein 14% carbohydrate Thermothelomyces heterothallicus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.26 purified to homogeneity using acetone precipitation followed by ion-exchange and gel-filtration column chromatography Thermothelomyces heterothallicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.26 4-nitrophenyl phosphate + H2O
-
Thermothelomyces heterothallicus 4-nitrophenol + phosphate
-
?
3.1.3.26 myo-inositol hexakisphosphate + H2O The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate Thermothelomyces heterothallicus ? + phosphate
-
?
3.1.3.26 phosphoenol pyruvate + H2O
-
Thermothelomyces heterothallicus ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.3.26 homopentamer 4 * 90000, SDS-PAGE, gel filtration Thermothelomyces heterothallicus

Synonyms

EC Number Synonyms Comment Organism
3.1.3.26 phytase
-
Thermothelomyces heterothallicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.26 60
-
-
Thermothelomyces heterothallicus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.3.26 additional information
-
T1/2 of 16 h at 60°C and 1.5 h at 80°C Thermothelomyces heterothallicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.26 5
-
-
Thermothelomyces heterothallicus