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Literature summary extracted from
- Salt effect on the pH profile and kinetic parameters of microbial phytases (2008), J. Agric. Food Chem., 56, 3398-3402.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.26 | overexpressed in Pichia pastoris | Escherichia coli |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.26 | NaF | - |
Aspergillus niger |  |
| 3.1.3.26 | NaF | - |
Escherichia coli | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.26 | 0.033 | - |
myo-inositol hexakisphosphate | pH 5.0, no salt | Aspergillus niger | |
| 3.1.3.26 | 0.036 | - |
myo-inositol hexakisphosphate | pH 4.0, no salt | Aspergillus niger | |
| 3.1.3.26 | 0.057 | - |
myo-inositol hexakisphosphate | pH 4.0, 0.5 M NaCl | Aspergillus niger | |
| 3.1.3.26 | 0.072 | - |
myo-inositol hexakisphosphate | pH 2.5, no salt | Escherichia coli | |
| 3.1.3.26 | 0.088 | - |
myo-inositol hexakisphosphate | pH 5.5, 0.5 M NaCl | Aspergillus niger | |
| 3.1.3.26 | 0.121 | - |
myo-inositol hexakisphosphate | pH 2.5, 0.5 M NaCl | Escherichia coli | |
| 3.1.3.26 | 0.2 | - |
myo-inositol hexakisphosphate | pH 6.0, no salt | Escherichia coli | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.26 | Ca2+ | extends the pH range to 8.0 | Aspergillus niger | |
| 3.1.3.26 | Na+ | a high concentration of sodium chloride affects the activity of fungal phytase in the pH 3-4 range | Aspergillus niger | |
| 3.1.3.26 | Na+ | a high concentration of sodium chloride shifts the pH optimum to 2.0 from 5.5 | Escherichia coli | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.26 | Aspergillus niger | - |
the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
| 3.1.3.26 | Escherichia coli | P07102 | the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.3.26 | using a MacroPrep S column | Escherichia coli |
| 3.1.3.26 | using ion-exchange columns Macroprep S and Q columns | Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus niger | ? + phosphate | - |
? | |
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Escherichia coli | ? + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.26 | PhyA | - |
Aspergillus niger |
| 3.1.3.26 | phytase | - |
Aspergillus niger |
| 3.1.3.26 | phytase | - |
Escherichia coli |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.26 | 37 | - |
assay at | Aspergillus niger |
| 3.1.3.26 | 37 | - |
assay at | Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.26 | 389 | - |
myo-inositol hexakisphosphate | pH 4.0, no salt | Aspergillus niger | |
| 3.1.3.26 | 745 | - |
myo-inositol hexakisphosphate | pH 5.0, no salt | Aspergillus niger | |
| 3.1.3.26 | 1037 | - |
myo-inositol hexakisphosphate | pH 4.0, 0.5 M NaCl | Aspergillus niger | |
| 3.1.3.26 | 1060 | - |
myo-inositol hexakisphosphate | pH 2.5, 0.5 M NaCl | Escherichia coli | |
| 3.1.3.26 | 1167 | - |
myo-inositol hexakisphosphate | pH 2.5, no salt | Escherichia coli | |
| 3.1.3.26 | 1305 | - |
myo-inositol hexakisphosphate | pH 5.5, 0.5 M NaCl | Aspergillus niger | |
| 3.1.3.26 | 1857 | - |
myo-inositol hexakisphosphate | pH 6.0, no salt | Escherichia coli | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.26 | 2 | - |
a high concentration of sodium chloride shifts the pH optimum to 2.0 from 5.5 | Escherichia coli |
| 3.1.3.26 | 5.5 | - |
- |
Escherichia coli |
| 3.1.3.26 | 5.5 | - |
assay at | Aspergillus niger |
| 3.1.3.26 | 5.5 | - |
assay at | Escherichia coli |
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