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Literature summary extracted from
- Expression and characterization of Aspergillus thermostable phytases in Pichia pastoris (2009), FEMS Microbiol. Lett., 290, 18-24.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | nutrition | enzyme is at least as efficient as commercial phytase for hydrolyzing phytate in corn-based animal feed | Aspergillus japonicus |
| 3.1.3.8 | nutrition | enzyme is at least as efficient as commercial phytase for hydrolyzing phytate in corn-based animal feed | Aspergillus niger |
| 3.1.3.26 | biotechnology | in vitro digestibility tests show recombinantly expressed phytase is at least as efficient as commercial phytase for hydrolyzing phytate in corn-based animal feed and is therefore suitable sources of phytase supplement | Aspergillus japonicus |
| 3.1.3.26 | biotechnology | in vitro digestibility tests show recombinantly expressed phytase is at least as efficient as commercial phytase for hydrolyzing phytate in corn-based animal feed and is therefore suitable sources of phytase supplement | Aspergillus niger |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.3.8 | expression in Pichia pastoris | Aspergillus japonicus |
| 3.1.3.8 | expression in Pichia pastoris | Aspergillus niger |
| 3.1.3.26 | recombinantly expressed as active extracellular, glycosylated proteins in Pichia pastoris | Aspergillus japonicus |
| 3.1.3.26 | recombinantly expressed as active extracellular, glycosylated proteins in Pichia pastoris | Aspergillus niger |
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.3.8 | enzyme is resistant to pepsin | Aspergillus japonicus |
| 3.1.3.8 | enzyme is resistant to pepsin | Aspergillus niger |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 0.08 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus japonicus |  |
| 3.1.3.8 | 0.14 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus japonicus | |
| 3.1.3.8 | 0.15 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.8 | 0.16 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.26 | 0.08 | - |
myo-inositol hexakisphosphate | phytic acid dipotassium salt | Aspergillus japonicus | |
| 3.1.3.26 | 0.14 | - |
myo-inositol hexakisphosphate | phytic acid sodium salt | Aspergillus japonicus | |
| 3.1.3.26 | 0.15 | - |
myo-inositol hexakisphosphate | phytic acid sodium salt | Aspergillus niger | |
| 3.1.3.26 | 0.16 | - |
myo-inositol hexakisphosphate | phytic acid dipotassium salt | Aspergillus niger | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.1.3.8 | extracellular | recombinant protein, expressed in Pichia pastoris | Aspergillus japonicus | - |
- |
| 3.1.3.8 | extracellular | recombinant protein, expressed in Pichia pastoris | Aspergillus niger | - |
- |
| 3.1.3.26 | extracellular | - |
Aspergillus japonicus | - |
- |
| 3.1.3.26 | extracellular | - |
Aspergillus niger | - |
- |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.3.26 | Ca2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Ca2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Cu2+ | inhibitory effect | Aspergillus japonicus | |
| 3.1.3.26 | Cu2+ | inhibitory effect | Aspergillus niger | |
| 3.1.3.26 | Fe2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Fe2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Mg2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Mg2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Mn2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Mn2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Ni2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Ni2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Sr2+ | stimulatory effect | Aspergillus japonicus | |
| 3.1.3.26 | Sr2+ | stimulatory effect | Aspergillus niger | |
| 3.1.3.26 | Zn2+ | inhibitory effect | Aspergillus japonicus | |
| 3.1.3.26 | Zn2+ | inhibitory effect | Aspergillus niger | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.26 | 66000 | - |
SDS-PAGE | Aspergillus japonicus |
| 3.1.3.26 | 66000 | - |
SDS-PAGE | Aspergillus niger |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.3.8 | Aspergillus japonicus | B3VPB2 | - |
- |
| 3.1.3.8 | Aspergillus japonicus BCC18313 | B3VPB2 | - |
- |
| 3.1.3.8 | Aspergillus niger | B3VPB3 | - |
- |
| 3.1.3.8 | Aspergillus niger BCC18081 | B3VPB3 | - |
- |
| 3.1.3.26 | Aspergillus japonicus | B3VPB2 | BCC18313 (TR86), the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
| 3.1.3.26 | Aspergillus niger | B3VPB3 | BCC18081 (TR170), the enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | - |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.3.26 | glycoprotein | - |
Aspergillus japonicus |
| 3.1.3.26 | glycoprotein | - |
Aspergillus niger |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.3.8 | additional information | no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s | Aspergillus niger | ? | - |
? | |
| 3.1.3.8 | additional information | no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s | Aspergillus japonicus | ? | - |
? | |
| 3.1.3.8 | additional information | no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.1 per mM and s | Aspergillus japonicus BCC18313 | ? | - |
? | |
| 3.1.3.8 | additional information | no substrate: 4-nitrophenyl phosphate, catalytic efficiency is about 0.04 per mM and s | Aspergillus niger BCC18081 | ? | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate disodium salt + H2O | - |
Aspergillus japonicus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate disodium salt + H2O | - |
Aspergillus niger | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate disodium salt + H2O | - |
Aspergillus japonicus BCC18313 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate disodium salt + H2O | - |
Aspergillus niger BCC18081 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate sodium salt + H2O | - |
Aspergillus japonicus | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate sodium salt + H2O | - |
Aspergillus niger | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate sodium salt + H2O | - |
Aspergillus japonicus BCC18313 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.8 | myo-inositol hexakisphosphate sodium salt + H2O | - |
Aspergillus niger BCC18081 | 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + 1D-myo-inositol-1,2,5,6 tetrakisphosphate + sodium phosphate | - |
? | |
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus japonicus | ? + phosphate | - |
? | |
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus niger | ? + phosphate | - |
? | |
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus japonicus BCC18313 | ? + phosphate | - |
? | |
| 3.1.3.26 | myo-inositol hexakisphosphate + H2O | The enzyme may be a 3-phytase, EC 3.1.3.8, or a 4-phytase (synonym 6-phytase, EC 3.1.3.26). The product of the hydrolysis of myo-inositol hexakisphosphate to 1D-myo-inositol 1,2,4,5,6-pentakisphosphate (3-phytase) or 1D-myo-inositol 1,2,3,5,6-pentakisphosphate (4-phytase) (i.e. 1L-myo-inositol 1,2,3,4,5-pentakisphosphate if 1L numbering is applied) has not been analyzed. The reaction was monitored by analyzing the released phosphate | Aspergillus niger BCC18081 | ? + phosphate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.3.8 | PhyA | - |
Aspergillus japonicus |
| 3.1.3.8 | PhyA | - |
Aspergillus niger |
| 3.1.3.26 | phytase | - |
Aspergillus japonicus |
| 3.1.3.26 | phytase | - |
Aspergillus niger |
| 3.1.3.26 | r-PhyA170 | - |
Aspergillus niger |
| 3.1.3.26 | r-PhyA86 | - |
Aspergillus japonicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 50 | - |
- |
Aspergillus japonicus |
| 3.1.3.8 | 50 | - |
- |
Aspergillus niger |
| 3.1.3.26 | 50 | - |
- |
Aspergillus japonicus |
| 3.1.3.26 | 50 | - |
- |
Aspergillus niger |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 100 | - |
10 min, more than 50% of iniital activity | Aspergillus japonicus |
| 3.1.3.8 | 100 | - |
10 min, more than 50% of iniital activity | Aspergillus niger |
| 3.1.3.26 | additional information | - |
more than 50% activity is retained after heating at 100°C for 10 min | Aspergillus japonicus |
| 3.1.3.26 | additional information | - |
more than 50% activity is retained after heating at 100°C for 10 min | Aspergillus niger |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 231 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus japonicus | |
| 3.1.3.8 | 253 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.8 | 279 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus japonicus | |
| 3.1.3.8 | 281 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.26 | 231 | - |
myo-inositol hexakisphosphate | phytic acid sodium salt | Aspergillus japonicus | |
| 3.1.3.26 | 253.5 | - |
myo-inositol hexakisphosphate | phytic acid dipotassium salt | Aspergillus niger | |
| 3.1.3.26 | 279.3 | - |
myo-inositol hexakisphosphate | phytic acid dipotassium salt | Aspergillus japonicus | |
| 3.1.3.26 | 281 | - |
myo-inositol hexakisphosphate | phytic acid sodium salt | Aspergillus niger | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 5.5 | - |
- |
Aspergillus japonicus |
| 3.1.3.8 | 5.5 | - |
- |
Aspergillus niger |
| 3.1.3.26 | 5.5 | - |
- |
Aspergillus japonicus |
| 3.1.3.26 | 5.5 | - |
- |
Aspergillus niger |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.3.8 | 2 | 8 | - |
Aspergillus japonicus |
| 3.1.3.8 | 2 | 8 | - |
Aspergillus niger |
| 3.1.3.26 | 2 | 8 | - |
Aspergillus japonicus |
| 3.1.3.26 | 2 | 8 | - |
Aspergillus niger |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.1.3.8 | 1584 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.8 | 1651 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus japonicus | |
| 3.1.3.8 | 1874 | - |
myo-inositol hexakisphosphate disodium salt | pH 5.5, 50°C | Aspergillus niger | |
| 3.1.3.8 | 3491 | - |
myo-inositol hexakisphosphate sodium salt | pH 5.5, 50°C | Aspergillus japonicus | |
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