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Literature summary extracted from

  • Raiger-Iustman, L.J.; Ruiz, J.A.
    The alternative sigma factor, sigmaS, affects polyhydroxyalkanoate metabolism in Pseudomonas putida (2008), FEMS Microbiol. Lett., 284, 218-224.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.76 a stationary sigma factor (rpoS)negative mutant is constructed to evaluate polyhydroxyalkanoate accumulation and expression of a translational fusion to the promoter region of the genes that code for polyhydroxyalkanoate synthase 1 (phaC1) and polyhydroxyalkanoate depolymerase (phaZ). The rpoS gene of Pseudomaonas putida KT2440 and its promoter region are amplified with primers and cloned into pBBR1MCS-5 to obtain pBBRrpoS. This plasmid is transferred to Pseudomonas putida SDM75 and SDMPC1. Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.76 additional information Pseudomonas putida Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted. ?
-
 ?
3.1.1.76 additional information Pseudomonas putida The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase. ?
-
 ?
3.1.1.76 additional information Pseudomonas putida the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate. ?
-
 ?
3.1.1.76 additional information Pseudomonas putida KT 2240 Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted. ?
-
 ?
3.1.1.76 additional information Pseudomonas putida KT 2240 The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase. ?
-
 ?
3.1.1.76 additional information Pseudomonas putida KT 2240 the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate. ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Pseudomonas putida
-
-
-
3.1.1.76 Pseudomonas putida KT 2240
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 additional information Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted. Pseudomonas putida ?
-
 ?
3.1.1.76 additional information The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase. Pseudomonas putida ?
-
 ?
3.1.1.76 additional information the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate. Pseudomonas putida ?
-
 ?
3.1.1.76 additional information Enhanced depolymerization obtained in the mutant could be attributable to the higher depolymerase gene (phaZ) expression and the fact that during the stationary phase the carbon source for polyhydroxyalkanoate synthesis is depleted. Pseudomonas putida KT 2240 ?
-
 ?
3.1.1.76 additional information The genes phaC1 and phaC2, which encode two polyhydroxyalkanoate polymerases, are separated by phaZ, which encodes a polyhydroxyalkanoate depolymerase. Pseudomonas putida KT 2240 ?
-
 ?
3.1.1.76 additional information the stationary sigma factor might control the genes involved in polyhydroxyalkanoate metabolism. The inactivation of the rpoS gene increases the polyhydroxyalkanoate degradation rate. Pseudomonas putida KT 2240 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 PhaZ
-
 Pseudomonas putida
3.1.1.76 polyhydroxyalkanoate depolymerase
-
 Pseudomonas putida