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Literature summary extracted from

  • Empadinhas, N.; Albuquerque, L.; Mendes, V.; Macedo-Ribeiro, S.; da Costa, M.S.
    Identification of the mycobacterial glucosyl-3-phosphoglycerate synthase (2008), FEMS Microbiol. Lett., 280, 195-202.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.266 gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree Mycobacterium tuberculosis
2.4.1.266 gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 Mycobacterium tuberculosis variant bovis
2.4.1.266 gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 Mycolicibacterium smegmatis
2.4.1.266 overexpressed in Escherichia coli as a C-terminal His-tagged protein Mycobacterium tuberculosis variant bovis
2.4.1.266 overexpressed in Escherichia coli as a C-terminal His-tagged protein Mycolicibacterium smegmatis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.266 additional information
-
additional information GpgSs exhibits Michaelis-Menten kinetics at 37°C Mycobacterium tuberculosis variant bovis
2.4.1.266 additional information
-
additional information GpgSs exhibits Michaelis-Menten kinetics at 37°C Mycolicibacterium smegmatis
2.4.1.266 0.24
-
3-phospho-D-glycerate pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
2.4.1.266 0.24
-
3-phospho-D-glycerate pH 7.5, 37°C Mycolicibacterium smegmatis
2.4.1.266 1.28
-
UDP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis
2.4.1.266 2.43
-
UDP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
2.4.1.266 6.08
-
ADP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis
2.4.1.266 6.63
-
ADP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
2.4.1.266 27.7
-
GDP-glucose pH 7.5, 37°C Mycobacterium tuberculosis variant bovis
2.4.1.266 32.04
-
GDP-glucose pH 7.5, 37°C Mycolicibacterium smegmatis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.266 Mg2+ strictly dependent on Mycobacterium tuberculosis
2.4.1.266 Mg2+ strictly dependent on Mycobacterium tuberculosis variant bovis
2.4.1.266 Mg2+ strictly dependent on Mycolicibacterium smegmatis
2.4.1.266 Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20 mM Mycolicibacterium smegmatis
2.4.1.266 Mg2+ activity is strictly dependent on Mg2+, maximal activation at 20-75 mM Mycobacterium tuberculosis variant bovis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.266 33900
-
3 * 33900, calculated from sequence Mycolicibacterium smegmatis
2.4.1.266 34400
-
3 * 34400, calculated from sequence Mycobacterium tuberculosis variant bovis
2.4.1.266 101100
-
gel filtration Mycobacterium tuberculosis variant bovis
2.4.1.266 106900
-
gel filtration Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate Mycobacterium tuberculosis variant bovis involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate Mycolicibacterium smegmatis involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate Mycolicibacterium smegmatis ATCC 700084 involved in biosynthesis of methylated polysaccharides UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.266 Mycobacterium tuberculosis
-
gene gpgS or Rv1208
-
2.4.1.266 Mycobacterium tuberculosis H37Rv
-
gene gpgS or Rv1208
-
2.4.1.266 Mycobacterium tuberculosis variant bovis
-
-
-
2.4.1.266 Mycobacterium tuberculosis variant bovis
-
slow-growing strain BCG, gene gpgS
-
2.4.1.266 Mycolicibacterium smegmatis A0R2E6 fast-growing strain 1102, gene gpgS
-
2.4.1.266 Mycolicibacterium smegmatis A0R2E6 strain 1102
-
2.4.1.266 Mycolicibacterium smegmatis ATCC 700084 A0R2E6 strain 1102
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.266 recombinant enzyme from Escherichia coli Mycobacterium tuberculosis variant bovis
2.4.1.266 recombinant enzyme from Escherichia coli Mycolicibacterium smegmatis
2.4.1.266 recombinant GpgS protein Mycobacterium tuberculosis variant bovis
2.4.1.266 recombinant GpgS protein Mycolicibacterium smegmatis

Storage Stability

EC Number Storage Stability Organism
2.4.1.266 0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month Mycobacterium tuberculosis variant bovis
2.4.1.266 0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month Mycolicibacterium smegmatis
2.4.1.266 22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week Mycobacterium tuberculosis variant bovis
2.4.1.266 22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
Mycobacterium tuberculosis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
Mycobacterium tuberculosis variant bovis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
Mycolicibacterium smegmatis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
Mycolicibacterium smegmatis ATCC 700084 ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-alpha-D-glucose + D-3-phosphoglycerate
-
Mycobacterium tuberculosis H37Rv ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
Mycobacterium tuberculosis variant bovis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 ADP-glucose + 3-phospho-D-glycerate
-
Mycolicibacterium smegmatis ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
Mycobacterium tuberculosis variant bovis GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
Mycolicibacterium smegmatis GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 GDP-glucose + 3-phospho-D-glycerate
-
Mycolicibacterium smegmatis ATCC 700084 GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
Mycobacterium tuberculosis variant bovis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
Mycolicibacterium smegmatis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycobacterium tuberculosis variant bovis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycolicibacterium smegmatis UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate
-
Mycolicibacterium smegmatis ATCC 700084 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + 3-phospho-D-glycerate involved in biosynthesis of methylated polysaccharides Mycolicibacterium smegmatis ATCC 700084 UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate
-
?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis variant bovis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate best substrates Mycolicibacterium smegmatis UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate best substrates Mycolicibacterium smegmatis ATCC 700084 UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
?
2.4.1.266 UDP-glucose + D-3-phosphoglycerate best substrates Mycobacterium tuberculosis H37Rv UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.266 trimer 3 * 33900, calculated from sequence Mycolicibacterium smegmatis
2.4.1.266 trimer 3 * 34400, calculated from sequence Mycobacterium tuberculosis variant bovis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.266 glucosyl-3-phosphoglycerate synthase
-
Mycobacterium tuberculosis
2.4.1.266 glucosyl-3-phosphoglycerate synthase
-
Mycobacterium tuberculosis variant bovis
2.4.1.266 glucosyl-3-phosphoglycerate synthase
-
Mycolicibacterium smegmatis
2.4.1.266 GpgS
-
Mycobacterium tuberculosis
2.4.1.266 GpgS
-
Mycobacterium tuberculosis variant bovis
2.4.1.266 GpgS
-
Mycolicibacterium smegmatis
2.4.1.266 GpgS protein
-
Mycobacterium tuberculosis variant bovis
2.4.1.266 GpgS protein
-
Mycolicibacterium smegmatis
2.4.1.266 More the enzyme belongs to the retaining family 81 glycosyltransferases Mycobacterium tuberculosis
2.4.1.266 More the enzyme belongs to the retaining family 81 glycosyltransferases Mycobacterium tuberculosis variant bovis
2.4.1.266 More the enzyme belongs to the retaining family 81 glycosyltransferases Mycolicibacterium smegmatis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.266 45
-
-
Mycobacterium tuberculosis
2.4.1.266 45
-
-
Mycobacterium tuberculosis variant bovis
2.4.1.266 45
-
-
Mycolicibacterium smegmatis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.266 20 55 no activity below 20°C and above 55°C Mycobacterium tuberculosis
2.4.1.266 20 55 no activity below 20°C and above 55°C Mycobacterium tuberculosis variant bovis
2.4.1.266 20 55 no activity below 20°C and above 55°C Mycolicibacterium smegmatis
2.4.1.266 30 50 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity Mycolicibacterium smegmatis
2.4.1.266 35 50 30°C: about 35% of maximal activity, 35°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity Mycobacterium tuberculosis variant bovis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.266 37
-
half-life value for inactivation of GpgS is 12.42 h Mycobacterium tuberculosis variant bovis
2.4.1.266 37
-
half-life value for inactivation of GpgS is 7.28 h Mycolicibacterium smegmatis
2.4.1.266 37
-
half-life 7.28 h Mycolicibacterium smegmatis
2.4.1.266 37
-
half-life: 12.42 h Mycobacterium tuberculosis variant bovis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.266 8
-
-
Mycobacterium tuberculosis
2.4.1.266 8
-
-
Mycobacterium tuberculosis variant bovis
2.4.1.266 8
-
-
Mycolicibacterium smegmatis
2.4.1.266 8.5
-
-
Mycolicibacterium smegmatis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.266 6 9 pH 6.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity Mycobacterium tuberculosis variant bovis
2.4.1.266 6 9.5 pH 6.0: about 55% of maximal activity, pH 9.5: about 60% of maximal activity Mycolicibacterium smegmatis
2.4.1.266 6 10 activity range Mycobacterium tuberculosis variant bovis
2.4.1.266 6 10 activity range Mycolicibacterium smegmatis