EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.1.266 | gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree | Mycobacterium tuberculosis |
2.4.1.266 | gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | gene gpgS, DNA and amino acid sequence determination and analysis, phylogenetic tree, expression in Escherichia coli strain BL21 | Mycolicibacterium smegmatis |
2.4.1.266 | overexpressed in Escherichia coli as a C-terminal His-tagged protein | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | overexpressed in Escherichia coli as a C-terminal His-tagged protein | Mycolicibacterium smegmatis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.266 | additional information | - |
additional information | GpgSs exhibits Michaelis-Menten kinetics at 37°C | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | additional information | - |
additional information | GpgSs exhibits Michaelis-Menten kinetics at 37°C | Mycolicibacterium smegmatis | |
2.4.1.266 | 0.24 | - |
3-phospho-D-glycerate | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | 0.24 | - |
3-phospho-D-glycerate | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
2.4.1.266 | 1.28 | - |
UDP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
2.4.1.266 | 2.43 | - |
UDP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | 6.08 | - |
ADP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis | |
2.4.1.266 | 6.63 | - |
ADP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | 27.7 | - |
GDP-glucose | pH 7.5, 37°C | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | 32.04 | - |
GDP-glucose | pH 7.5, 37°C | Mycolicibacterium smegmatis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.266 | Mg2+ | strictly dependent on | Mycobacterium tuberculosis | |
2.4.1.266 | Mg2+ | strictly dependent on | Mycobacterium tuberculosis variant bovis | |
2.4.1.266 | Mg2+ | strictly dependent on | Mycolicibacterium smegmatis | |
2.4.1.266 | Mg2+ | activity is strictly dependent on Mg2+, maximal activation at 20 mM | Mycolicibacterium smegmatis | |
2.4.1.266 | Mg2+ | activity is strictly dependent on Mg2+, maximal activation at 20-75 mM | Mycobacterium tuberculosis variant bovis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 33900 | - |
3 * 33900, calculated from sequence | Mycolicibacterium smegmatis |
2.4.1.266 | 34400 | - |
3 * 34400, calculated from sequence | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 101100 | - |
gel filtration | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 106900 | - |
gel filtration | Mycolicibacterium smegmatis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | Mycobacterium tuberculosis variant bovis | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | Mycolicibacterium smegmatis | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | Mycolicibacterium smegmatis ATCC 700084 | involved in biosynthesis of methylated polysaccharides | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.266 | Mycobacterium tuberculosis | - |
gene gpgS or Rv1208 | - |
2.4.1.266 | Mycobacterium tuberculosis H37Rv | - |
gene gpgS or Rv1208 | - |
2.4.1.266 | Mycobacterium tuberculosis variant bovis | - |
- |
- |
2.4.1.266 | Mycobacterium tuberculosis variant bovis | - |
slow-growing strain BCG, gene gpgS | - |
2.4.1.266 | Mycolicibacterium smegmatis | A0R2E6 | fast-growing strain 1102, gene gpgS | - |
2.4.1.266 | Mycolicibacterium smegmatis | A0R2E6 | strain 1102 | - |
2.4.1.266 | Mycolicibacterium smegmatis ATCC 700084 | A0R2E6 | strain 1102 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.266 | recombinant enzyme from Escherichia coli | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | recombinant enzyme from Escherichia coli | Mycolicibacterium smegmatis |
2.4.1.266 | recombinant GpgS protein | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | recombinant GpgS protein | Mycolicibacterium smegmatis |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.266 | 0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 0°C, purified recombinant enzyme, nearly 100% remaining activity after 1 month | Mycolicibacterium smegmatis |
2.4.1.266 | 22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 22°C, room temperature, purified recombinant enzyme, nearly 100% remaining activity after 1 week | Mycolicibacterium smegmatis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.266 | ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis variant bovis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycolicibacterium smegmatis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-alpha-D-glucose + D-3-phosphoglycerate | - |
Mycobacterium tuberculosis H37Rv | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | ADP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | ADP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | GDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | GDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | - |
Mycobacterium tuberculosis variant bovis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycobacterium tuberculosis variant bovis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycolicibacterium smegmatis | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | - |
Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + 3-phospho-D-glycerate | involved in biosynthesis of methylated polysaccharides | Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-O-(alpha-D-glucopyranosyl)-3-phospho-D-glycerate | - |
? | |
2.4.1.266 | UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
2.4.1.266 | UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis variant bovis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
2.4.1.266 | UDP-glucose + D-3-phosphoglycerate | best substrates | Mycolicibacterium smegmatis | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
2.4.1.266 | UDP-glucose + D-3-phosphoglycerate | best substrates | Mycolicibacterium smegmatis ATCC 700084 | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? | |
2.4.1.266 | UDP-glucose + D-3-phosphoglycerate | best substrates | Mycobacterium tuberculosis H37Rv | UDP + 2-(beta-D-glucosyl)-sn-glycerol 3-phosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.1.266 | trimer | 3 * 33900, calculated from sequence | Mycolicibacterium smegmatis |
2.4.1.266 | trimer | 3 * 34400, calculated from sequence | Mycobacterium tuberculosis variant bovis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.266 | glucosyl-3-phosphoglycerate synthase | - |
Mycobacterium tuberculosis |
2.4.1.266 | glucosyl-3-phosphoglycerate synthase | - |
Mycobacterium tuberculosis variant bovis |
2.4.1.266 | glucosyl-3-phosphoglycerate synthase | - |
Mycolicibacterium smegmatis |
2.4.1.266 | GpgS | - |
Mycobacterium tuberculosis |
2.4.1.266 | GpgS | - |
Mycobacterium tuberculosis variant bovis |
2.4.1.266 | GpgS | - |
Mycolicibacterium smegmatis |
2.4.1.266 | GpgS protein | - |
Mycobacterium tuberculosis variant bovis |
2.4.1.266 | GpgS protein | - |
Mycolicibacterium smegmatis |
2.4.1.266 | More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycobacterium tuberculosis |
2.4.1.266 | More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | More | the enzyme belongs to the retaining family 81 glycosyltransferases | Mycolicibacterium smegmatis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 45 | - |
- |
Mycobacterium tuberculosis |
2.4.1.266 | 45 | - |
- |
Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 45 | - |
- |
Mycolicibacterium smegmatis |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 20 | 55 | no activity below 20°C and above 55°C | Mycobacterium tuberculosis |
2.4.1.266 | 20 | 55 | no activity below 20°C and above 55°C | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 20 | 55 | no activity below 20°C and above 55°C | Mycolicibacterium smegmatis |
2.4.1.266 | 30 | 50 | 30°C: about 40% of maximal activity, 50°C: about 70% of maximal activity | Mycolicibacterium smegmatis |
2.4.1.266 | 35 | 50 | 30°C: about 35% of maximal activity, 35°C: about 60% of maximal activity, 50°C: about 90% of maximal activity, 55°C: about 25% of maximal activity | Mycobacterium tuberculosis variant bovis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 37 | - |
half-life value for inactivation of GpgS is 12.42 h | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 37 | - |
half-life value for inactivation of GpgS is 7.28 h | Mycolicibacterium smegmatis |
2.4.1.266 | 37 | - |
half-life 7.28 h | Mycolicibacterium smegmatis |
2.4.1.266 | 37 | - |
half-life: 12.42 h | Mycobacterium tuberculosis variant bovis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 8 | - |
- |
Mycobacterium tuberculosis |
2.4.1.266 | 8 | - |
- |
Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 8 | - |
- |
Mycolicibacterium smegmatis |
2.4.1.266 | 8.5 | - |
- |
Mycolicibacterium smegmatis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.266 | 6 | 9 | pH 6.0: about 50% of maximal activity, pH 9.5: about 75% of maximal activity | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 6 | 9.5 | pH 6.0: about 55% of maximal activity, pH 9.5: about 60% of maximal activity | Mycolicibacterium smegmatis |
2.4.1.266 | 6 | 10 | activity range | Mycobacterium tuberculosis variant bovis |
2.4.1.266 | 6 | 10 | activity range | Mycolicibacterium smegmatis |