EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.4.6 | gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Pseudomonas sp. |
2.7.4.6 | gene ndk, expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha | Halomonas sp. #593 |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.4.6 | A134E | site-directed mutagenesis, the mutant forms a dimer in analogy to the wild-type enzyme of Halomonas sp., which possesses an E134 residue, overview | Pseudomonas sp. |
2.7.4.6 | E134A | site-directed mutagenesis, the mutant forms a tetramer in analogy to the wild-type enzyme of Pseudomonas sp., which possesses an A134 residue, overview | Halomonas sp. #593 |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.4.6 | Mg2+ | - |
Pseudomonas sp. | |
2.7.4.6 | Mg2+ | - |
Halomonas sp. #593 |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.4.6 | 15592 | - |
2 * 15592 | Halomonas sp. #593 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.6 | ATP + TDP | Pseudomonas sp. | - |
ADP + TTP | - |
r | |
2.7.4.6 | ATP + TDP | Halomonas sp. #593 | - |
ADP + TTP | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.4.6 | Halomonas sp. #593 | Q83WH5 | gene ndk | - |
2.7.4.6 | Pseudomonas sp. | - |
gene ndk | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.4.6 | ATP + TDP | - |
Pseudomonas sp. | ADP + TTP | - |
r | |
2.7.4.6 | ATP + TDP | - |
Halomonas sp. #593 | ADP + TTP | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.4.6 | dimer | 2 * 15592 | Halomonas sp. #593 |
2.7.4.6 | More | structure modeling of wild-type and mutant enzyme, overview | Pseudomonas sp. |
2.7.4.6 | More | structure modeling of wild-type and mutant enzyme, overview | Halomonas sp. #593 |
2.7.4.6 | tetramer | - |
Pseudomonas sp. |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.4.6 | NDK | - |
Pseudomonas sp. |
2.7.4.6 | NDK | - |
Halomonas sp. #593 |
2.7.4.6 | nucleoside diphosphate kinase | - |
Pseudomonas sp. |
2.7.4.6 | nucleoside diphosphate kinase | - |
Halomonas sp. #593 |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.4.6 | 30 | - |
assay at | Pseudomonas sp. |
2.7.4.6 | 30 | - |
assay at | Halomonas sp. #593 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.4.6 | 7.5 | - |
assay at | Pseudomonas sp. |
2.7.4.6 | 7.5 | - |
assay at | Halomonas sp. #593 |