EC Number | Application | Comment | Organism |
---|---|---|---|
4.1.1.48 | medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis |
4.1.1.48 | medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis variant bovis BCG |
4.1.1.48 | medicine | IGPS may be a potential target for the development of anti-tuberculosis drugs | Mycobacterium tuberculosis H37Ra |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.48 | expressed in Escherichia coli strain BL21 | Mycobacterium tuberculosis |
4.1.1.48 | expressed in Escherichia coli strain BL21 | Mycobacterium tuberculosis H37Ra |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
4.1.1.48 | E168A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | E168A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | H170A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | H170A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | L193A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | L193A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | L196A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | L196A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | additional information | site directed mutagenesis studies indicate that ATB107 may inhibit IGPS activity by reducing the binding affinity for substrate of residues Glu168 and Asn189 | Mycobacterium tuberculosis |
4.1.1.48 | additional information | site directed mutagenesis studies indicate that ATB107 may inhibit IGPS activity by reducing the binding affinity for substrate of residues Glu168 and Asn189 | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | N189A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | N189A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | N192A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | N192A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | P63A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | P63A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | R191A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | R191A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | S64A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | S64A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
4.1.1.48 | V169A | site directed mutagenesis | Mycobacterium tuberculosis |
4.1.1.48 | V169A | site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.48 | ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis | |
4.1.1.48 | ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | ATB107 | 1-azabicyclo[2.2.2]octan-3-one[4-(phenylamino)-6-(1-piperidinyl)-1,3,5-triazin-2-yl]hydrazone, 0.1 microg/ml minimum inhibitory concentration, potent competitive inhibitor of the substrate 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | Mycobacterium tuberculosis variant bovis BCG | |
4.1.1.48 | L-tryptophan | tryptophan at high concentrations has definite inhibitory activity against Mycobacterium tuberculosis but does not antagonize the activity of ATB107 | Mycobacterium tuberculosis | |
4.1.1.48 | additional information | inhibitory effect of ATB107 can not be reversed by the addition of tryptophan | Mycobacterium tuberculosis | |
4.1.1.48 | additional information | inhibitory effect of ATB107 can not be reversed by the addition of tryptophan | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | tryptophan | tryptophan at high concentrations has definite inhibitory activity against Mycobacterium tuberculosis but does not antagonize the activity of ATB107 | Mycobacterium tuberculosis H37Ra |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.48 | 1.13 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | increased Km value in the presence of ATB107 | Mycobacterium tuberculosis | |
4.1.1.48 | 1.13 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | increased Km value in the presence of ATB107 | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 1.19 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | H170A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 1.19 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | H170A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 1.42 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L193A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 1.42 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L193A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | S64A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | S64A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | V169A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 1.53 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | V169A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 1.81 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L196A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 1.81 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | L196A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 2.49 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | P63A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 2.49 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | P63A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 2.57 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N192A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 2.57 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N192A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 6.63 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | R191A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 6.63 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | R191A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 20.34 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N189A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 20.34 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | N189A site directed mutagenesis | Mycobacterium tuberculosis H37Ra | |
4.1.1.48 | 21.67 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | E168A site directed mutagenesis | Mycobacterium tuberculosis | |
4.1.1.48 | 21.67 | - |
1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate | E168A site directed mutagenesis | Mycobacterium tuberculosis H37Ra |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.48 | Mycobacterium tuberculosis | P9WFX7 | - |
- |
4.1.1.48 | Mycobacterium tuberculosis H37Ra | A5U2W7 | - |
- |
4.1.1.48 | Mycobacterium tuberculosis H37Rv | P9WFX7 | - |
- |
4.1.1.48 | Mycobacterium tuberculosis variant bovis BCG | A1KJ27 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis variant bovis BCG | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis H37Ra | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? | |
4.1.1.48 | 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate + ? | - |
Mycobacterium tuberculosis H37Rv | 1-C-(indol-3-yl)glycerol 3-phosphate + CO2 + H2O | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
4.1.1.48 | More | one typical (beta /alpha)8-barrel structure | Mycobacterium tuberculosis |
4.1.1.48 | More | one typical (beta /alpha)8-barrel structure | Mycobacterium tuberculosis H37Ra |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.48 | IGPS | - |
Mycobacterium tuberculosis |
4.1.1.48 | IGPS | - |
Mycobacterium tuberculosis variant bovis BCG |
4.1.1.48 | IGPS | - |
Mycobacterium tuberculosis H37Ra |
4.1.1.48 | indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis |
4.1.1.48 | indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis variant bovis BCG |
4.1.1.48 | indole-3-glycerol phosphate synthase | - |
Mycobacterium tuberculosis H37Ra |
4.1.1.48 | mIGPS | - |
Mycobacterium tuberculosis |
4.1.1.48 | mIGPS | - |
Mycobacterium tuberculosis variant bovis BCG |
4.1.1.48 | mIGPS | - |
Mycobacterium tuberculosis H37Ra |