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Literature summary extracted from
- Purification and partial characterization of the hydroxylase component of the methanesulfonic acid mono-oxygenase from Methylosulfonomonas methylovora strain M2 (2000), Eur. J. Biochem., 267, 4763-4769.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.14.13.111 | cloning of the reductase encoded by the msmD gene. Cloning and overexpression of the msmD gene, encoding the reductase component, as a GST fusion protein results in the expression of a 65000 Da polypeptide matching the size of the GST protein plus the reductase protein when induced with isopropyl thio-beta-D-galactoside. The fusion between the two proteins is unstable, and purification by affinity chromatography is not possible | Methylosulfonomonas methylovora |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.14.13.111 | cytoplasm | - |
Methylosulfonomonas methylovora | 5737 | - |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.111 | Iron | presence of iron-sulfur centres. The two-component hydroxylase of methanesulfonic acid monooxygenase preparations containes 1 mol sulfide and 3 mol iron per mol alphabeta-monomer | Methylosulfonomonas methylovora |  |
| 1.14.13.111 | additional information | chromium, cobalt, copper, lead, nickel, molybdenum, tungsten and vanadium are not detected in two-component hydroxylase of methanesulfonic acid monooxygenase | Methylosulfonomonas methylovora |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.111 | 23000 | - |
3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE | Methylosulfonomonas methylovora |
| 1.14.13.111 | 43700 | - |
3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE | Methylosulfonomonas methylovora |
| 1.14.13.111 | 209000 | - |
two-component hydroxylase of methanesulfonic acid monooxygenase, gel filtration | Methylosulfonomonas methylovora |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | Methylosulfonomonas methylovora | MSAMO is specifically induced during growth on methanesulfonate | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | Methylosulfonomonas methylovora M2 | MSAMO is specifically induced during growth on methanesulfonate | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.13.111 | Methylosulfonomonas methylovora | - |
- |
- |
| 1.14.13.111 | Methylosulfonomonas methylovora M2 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.14.13.111 | purification of two-component hydroxylase of methanesulfonic acid monooxygenase. Purification of the reductase component (MsmD) using a range of chromatographic techniques fails | Methylosulfonomonas methylovora |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.13.111 | 0.88 | - |
- |
Methylosulfonomonas methylovora |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | - |
Methylosulfonomonas methylovora | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | MSAMO is specifically induced during growth on methanesulfonate | Methylosulfonomonas methylovora | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | - |
Methylosulfonomonas methylovora M2 | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
| 1.14.13.111 | CH3SO3- + O2 + NADH + H+ | MSAMO is specifically induced during growth on methanesulfonate | Methylosulfonomonas methylovora M2 | formaldehyde + HSO3- + NAD+ + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.13.111 | hexamer | 3 * 43700 (MsmA) + 3 * 23000 (MsmB), two-component hydroxylase of methanesulfonic acid monooxygenase, SDS-PAGE | Methylosulfonomonas methylovora |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.14.13.111 | methanesulfonic acid monooxygenase | - |
Methylosulfonomonas methylovora |
| 1.14.13.111 | MSAMO | - |
Methylosulfonomonas methylovora |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.13.111 | additional information | flavin is absent in the two-component hydroxylase of methanesulfonic acid monooxygenase | Methylosulfonomonas methylovora | |
| 1.14.13.111 | NADH | - |
Methylosulfonomonas methylovora | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.13.111 | Methylosulfonomonas methylovora | two-component hydroxylase of methanesulfonic acid monooxygenase, chromatofocusing | - |
4.9 |
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