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Literature summary extracted from

  • Castle, S.A.; Owuor, E.A.; Thompson, S.H.; Garnsey, M.R.; Klutts, J.S.; Doering, T.L.; Levery, S.B.
    beta1,2-Xylosyltransferase Cxt1p is solely responsible for xylose incorporation into Cryptococcus neoformans glycosphingolipids (2008), Eukaryot. Cell, 7, 1611-1615.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
2.4.2.38 medicine development of diagnostic and therapeutic strategies due to importance of xylose transfer in cryptococcal biology, pathogenesis and virulence Cryptococcus neoformans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.38 additional information Cryptococcus neoformans preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1 ?
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 ?
2.4.2.38 additional information Cryptococcus neoformans transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids ?
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 ?
2.4.2.38 UDP-xylose + mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide Cryptococcus neoformans no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC, mannosyl-alpha-(1,3)-mannose-alpha motif also present in substrates for capsule polysaccharide synthesis UDP + mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide core structure of glycosylinositol phosphorylceramides, GIPCs ?
2.4.2.38 UDP-xylose + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide Cryptococcus neoformans no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC UDP + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
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 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.38 Cryptococcus neoformans Q5K8R6 serotype D, wild-type strain JEC21 (serotype D MATalpha) and mutant strain JEC21/cxt1delta (serotype D, isogenic MAT alpha, encapsulated and nourseothricin-resistant, lacking CXT1p)
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.38 additional information preferred substrate depends on wild-type strain, strain JEC21: both products detectable (mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide and mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide) at a ratio of approximately 1:1 Cryptococcus neoformans ?
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 ?
2.4.2.38 additional information transfer of beta (1,2)-xylose, synthesis of capsule polysaccharides and fungi-specific glycosphingolipids Cryptococcus neoformans ?
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 ?
2.4.2.38 UDP-xylose + mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC, mannosyl-alpha-(1,3)-mannose-alpha motif also present in substrates for capsule polysaccharide synthesis Cryptococcus neoformans UDP + mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide core structure of glycosylinositol phosphorylceramides, GIPCs ?
2.4.2.38 UDP-xylose + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide no transfer of xylose residues onto substrate in absence of Cxt1p (JEC21/cxt1delta) as revealed by mass spectrometry and HPTLC Cryptococcus neoformans UDP + mannosyl-alpha-(1,6)-mannosyl-alpha-(1,3)-[xylosyl-beta-(1,2)]-mannosyl-alpha-(1,4)-galactosyl-beta-(1,6)-mannosyl-alpha-(1,2)-inositol phosphorylceramide
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 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.38 beta-1,2-xylosyltransferase
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 Cryptococcus neoformans
2.4.2.38 CXT1
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 Cryptococcus neoformans
2.4.2.38 Cxt1p
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 Cryptococcus neoformans
2.4.2.38 Xyl-beta-1,2-transferase
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 Cryptococcus neoformans