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Literature summary extracted from

  • Bai, L.; Jiang, R.; Shan, J.; Guo, L.; Zhang, Y.; Zhang, R.; Li, Y.
    Purification, characterization and functional analysis of asparagine synthetase encoding by ste10 gene in Ebosin biosynthesis of Streptomyces sp. 139 (2008), Enzyme Microb. Technol., 42, 548-553.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.5.4 expression in Escherichia coli Streptomyces sp.

Protein Variants

EC Number Protein Variants Comment Organism
6.3.5.4 additional information after ste10 gene knock-out, the monosaccharide composition of the exopolysaccharide produced by the mutant is changed in comparison with that of native exopolysaccharide Ebosin while its antagonist activity for IL-1R decreases significantly Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.5.4 0.9
-
 L-Asp pH 7.4, 37°C Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.5.4 70000
-
 x * 70000, SDS-PAGE and calculated, His-tagged protein Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
6.3.5.4 Streptomyces sp. Q8KN11
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.5.4 recombinant protein Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.5.4 ATP + L-Asp + L-Gln
-
 Streptomyces sp. AMP + diphosphate + Asn + Glu
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.5.4 ? x * 70000, SDS-PAGE and calculated, His-tagged protein Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
6.3.5.4 Ste10
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.3.5.4 37
-
-
 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.3.5.4 7.4
-
-
 Streptomyces sp.