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Literature summary extracted from

  • Cheetham, P.S.; Hacking, A.J.; Vlitos, M.
    Synthesis of novel disaccharides by a newly isolated fructosyl transferase from Bacillus subtilis (1989), Enzyme Microb. Technol., 11, 212-219.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.162 additional information the fructosyl transferase is unaffected by anionic, cationic, or nonionic detergents such as SDS, cetyltrimethylammonium bromide, and Triton X-100 Bacillus subtilis
2.4.1.162 sucrose the fructosyl transferase activity in strain NCIB 11871 is induced only by sucrose, other sugars tested as inducers (glucose, fructose, maltose, and cellobiose) are ineffective Bacillus subtilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.162 D-glucose
-
Bacillus subtilis
2.4.1.162 D-mellibiose
-
Bacillus subtilis
2.4.1.162 additional information p-hydroxymercuribenzoate does not affect fructosyl transferase activity, the fructosyl transferase is completely resistant to endogenous proteases and to trypsin Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.162 700
-
sucrose using D-xylose as the acceptor sugar, at pH 5.4 and 30°C Bacillus subtilis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.162 additional information no requirement for metal ions Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.162 Bacillus subtilis
-
-
-
2.4.1.162 Bacillus subtilis NCIB 11871
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.162 ammonium sulfate precipitation, DEAE-52 cellulose column chromatography, and Mono Q column chromatography Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.162 additional information
-
the purified enzyme is concentrated 33fold and has an activity of 32 nmol/ml/min Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.162 1',6'-dichlorosucrose + fructose acceptor 1',6'-dichlorosucrose has some donor activity Bacillus subtilis ?
-
?
2.4.1.162 6,1',6'-trichlorosucrose + fructose acceptor 6, 1',6'-trichlorosucrose has some donor activity Bacillus subtilis ?
-
?
2.4.1.162 6,1',6'-trichlorosucrose + fructose acceptor 6, 1',6'-trichlorosucrose has some donor activity Bacillus subtilis NCIB 11871 ?
-
?
2.4.1.162 6-chlorosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 6-chlorosucrose + fructose acceptor
-
Bacillus subtilis NCIB 11871 ?
-
?
2.4.1.162 6-deoxysucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 6-deoxysucrose + fructose acceptor
-
Bacillus subtilis NCIB 11871 ?
-
?
2.4.1.162 D-sucrose + D-galactose 6-acetate
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-6-O-acetyl-galactopyranoside
-
?
2.4.1.162 D-sucrose + D-galactose 6-phosphate
-
Bacillus subtilis ?
-
?
2.4.1.162 D-sucrose + D-gluconic acid
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-gluconic acid
-
?
2.4.1.162 galactosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 additional information when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive Bacillus subtilis ?
-
?
2.4.1.162 additional information when oligosaccharide acceptors are used, the acceptor activity decreases with increasing size, as in the homologous series maltose, maltotriose, maltotetrose, etc., reactivity also decreases with the size of the molecule in the homologous series sucrose, raffinose, stachyose, and verbascose, inulin and levan, and also levan that is partially hydrolysed with acid, do not act as donors, lactulose, isomaltulose, D-glucose-1-phosphate, methyl beta-D-glucoside, and D-xylulose are unreactive Bacillus subtilis NCIB 11871 ?
-
?
2.4.1.162 raffinose + D-xylose
-
Bacillus subtilis beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside + alpha-D-galactopyranosyl alpha-D-glucopyranoside
-
?
2.4.1.162 stachyose + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + 1-thio-D-glucose
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + 3-O-methyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-3-O-methyl-D-glucoside
-
?
2.4.1.162 sucrose + 4-chloro-D-galactose
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + 5-thio-D-glucose
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + 6-chloro-6-deoxy-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-chloro-6-deoxy-D-glucoside
-
?
2.4.1.162 sucrose + 6-deoxy-D-glucose
-
Bacillus subtilis 6-deoxysucrose + D-glucose
-
?
2.4.1.162 sucrose + 6-O-benzyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-benzyl-D-glucoside
-
?
2.4.1.162 sucrose + 6-O-methyl-D-galactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-galactoside
-
?
2.4.1.162 sucrose + 6-O-methyl-D-glucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-6-O-methyl-D-glucoside
-
?
2.4.1.162 sucrose + cellobiose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-cellobioside
-
?
2.4.1.162 sucrose + D-arabinose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-arabinoside
-
?
2.4.1.162 sucrose + D-fucose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-fucoside
-
?
2.4.1.162 sucrose + D-galactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-galactopyranoside
-
?
2.4.1.162 sucrose + D-glucose 6-acetate
-
Bacillus subtilis sucrose 6-acetate + D-glucose
-
?
2.4.1.162 sucrose + D-glucose 6-phosphate
-
Bacillus subtilis sucrose 6-phosphate + D-glucose
-
?
2.4.1.162 sucrose + D-lactose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-lactoside
-
?
2.4.1.162 sucrose + D-lyxose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-lyxoside
-
?
2.4.1.162 sucrose + D-maltose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-maltoside
-
?
2.4.1.162 sucrose + D-mannose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-mannopyranoside
-
?
2.4.1.162 sucrose + D-ribose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-D-riboside
-
?
2.4.1.162 sucrose + D-xylose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-alpha-D-xylopyranoside
-
?
2.4.1.162 sucrose + D-xylose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-2,1-alpha-D-xylopyranoside
-
?
2.4.1.162 sucrose + ethyl maltol
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + glycerol
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + isomaltose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-isomaltoside
-
?
2.4.1.162 sucrose + L-arabinose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-L-arabinoside
-
?
2.4.1.162 sucrose + L-rhamnose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-L-rhamnoside
-
?
2.4.1.162 sucrose + L-sorbose
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + maltohexaose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltohexaoside
-
?
2.4.1.162 sucrose + maltol
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + maltopentaose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltopentaoside
-
?
2.4.1.162 sucrose + maltotetraose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-maltotetraoside
-
?
2.4.1.162 sucrose + maltulose
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + melibiose
-
Bacillus subtilis D-glucose + beta-D-fructofuranosyl-(2,1)-mellibioside
-
?
2.4.1.162 sucrose + xylitol
-
Bacillus subtilis ?
-
?
2.4.1.162 sucrose + xylitol
-
Bacillus subtilis NCIB 11871 ?
-
?
2.4.1.162 sucrose 6-acetate + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 verbascose + fructose acceptor
-
Bacillus subtilis ?
-
?
2.4.1.162 xylosucrose + fructose acceptor
-
Bacillus subtilis ?
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.162 beta-fructofuranoside, acceptor (aldose) fructosyltransferase
-
Bacillus subtilis
2.4.1.162 beta-fructosyl transferase
-
Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.162 40
-
the enzyme is stable up to 40°C Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.162 5
-
-
Bacillus subtilis