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Literature summary extracted from

  • Wittich, R.M.; Haidour, A.; Van Dillewijn, P.; Ramos, J.L.
    OYE flavoprotein reductases initiate the condensation of TNT-derived intermediates to secondary diarylamines and nitrite (2008), Environ. Sci. Technol., 42, 734-739.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.1.B1 For His-tagged protein production, plasmid is transformed into Escherichia coli BL21 pLysS. Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.1.B1 NH4Cl included in assay medium Pseudomonas putida
1.7.1.B1 Zn2+ included in assay medium Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.B1 Pseudomonas putida
-
-
-
1.7.1.B1 Pseudomonas putida JLR11
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.B1 by nickel affinity chromatography using a continuous imidazole gradient Pseudomonas putida

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.1.B1 2,4,6-trinitrotoluene + 2 NADPH + 2 H+ = N-hydroxy-2-methyl-3,5-dinitroaniline + 2 NADP+ + H2O The amount of N-hydroxy-2-methyl-3,5-dinitroaniline is larger than the amount of N-hydroxy-4-methyl-3,5-dinitroaniline formed. In a further nonenzymatic reaction nitrite is released and N-(2-methyl-3,5-dinitrophenyl)-N-4-methyl-3,5-dinitrophenyl-hydroxylamine and eventually N-(2-methyl-3,5-dinitrophenyl)-N-4-methyl-3,5-dinitroaniline are produced. In adidtion the aromatic ring of 2,4,6-trinitrotoluene is susceptible to nucleophilic attack by hydride ions to form Meisenheimer complex intermediates (monohydride and dihydride complexes) which also form the secondary diaryl hydroxylamines and the secondary diarylamine with release of nitrite Pseudomonas putida
a
1.7.1.B1 2,4,6-trinitrotoluene + 2 NADPH + 2 H+ = N-hydroxy-4-methyl-3,5-dinitroaniline + 2 NADP+ + H2O The amount of N-hydroxy-4-methyl-3,5-dinitroaniline is larger than the amount of N-hydroxy-2-methyl-3,5-dinitroaniline formed. In a further nonenzymatic reaction nitrite is released and N,N-bis-(3,5-dinitrotolyl)-hydroxylamine and eventually N,N-bis-(3,5-dinitrotolyl)-amine are produced. In addition the aromatic ring of 2,4,6-trinitrotoluene is susceptible to nucleophilic attack by hydride ions to form Meisenheimer complex intermediates (monohydride and dihydride complexes) which also form the secondary diaryl hydroxylamines and the secondary diarylamine with release of nitrite Pseudomonas putida
a

Synonyms

EC Number Synonyms Comment Organism
1.7.1.B1 flavoprotein reductase
-
 Pseudomonas putida
1.7.1.B1 xenobiotic reductase B
-
 Pseudomonas putida
1.7.1.B1 xenobiotic reductase B (XenB)-like flavin reductase
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.B1 25
-
 assay at Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.B1 7
-
 assay at Pseudomonas putida