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Literature summary extracted from

  • Putrins, M.; Ilves, H.; Kivisaar, M.; Horak, R.
    ColRS two-component system prevents lysis of subpopulation of glucose-grown Pseudomonas putida (2008), Environ. Microbiol., 10, 2886-2893.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.23 ColR and oprB1 knockouts are constructed and expressed in Escherichia coli Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Pseudomonas putida
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-
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Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 cell culture strain PaW85 (isogenic to fully sequenced KT2440) Pseudomonas putida
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 additional information
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 beta-galactosidase activity is barely detectable in supernatants of wild-type and oprB1-deficient strains, the supernatant of glucose-grown colR-deficient strain contains 4% of total beta-galactosidase activity of the cell suspension. Pseudomonas putida
3.2.1.23 additional information
-
 Membrane permeability of colRoprB1 double mutant is similar to that of the wild-type regardless of the presence of phenol in the growth medium. Pseudomonas putida
3.2.1.23 additional information
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 The oprB1 single mutant also behaves like the wild type in membrane permeability test. Pseudomonas putida
3.2.1.23 additional information
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 The presence of phenol in the growth medium of colR mutant increases the proportion of extracellular beta-galactosidase activity up to 15%. Pseudomonas putida
3.2.1.23 additional information
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 To evaluate membrane permeability, measurement of the activity of an intracellular enzyme beta-galactosidase in nonpermeabilized cells is done. beta-galactosidase is assayed after the bacterial cell membrane is permeabilized with SDS and chloroform. beta-galactosidase activity of untreated cells is compared with that of permeabilized cells. As a source of beta-galactosidase, a plasmid pKTlacZS/C containing the tnpA promoter of the transposon Tn4652 upstream the lacZ gene is used. beta-galactosidase activity in permeabilized glucose-grown cells of all studied genetic backgrounds is about 80 Miller units. Pseudomonas putida
3.2.1.23 additional information
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 Up to 15% of total beta-galactosidase activity is detectable in colR-deficient cells grown on glucose minimal plates, and about 35% when cells are grown on glucose medium supplemented with 1 mM phenol. The membrane of colR-deficient strain is more permeable and the presence of phenol further increases the leakiness of the membrane of colR mutant. Pseudomonas putida
3.2.1.23 additional information
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 When cells are assayed without the prior chloroform and SDS treatment, differences between the strains become evident. Only 4% of total beta-galactosidase activity is measurable in nonpermeabilized wild-type cells regardless of the presence of phenol in the growth medium. Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 beta-galactosidase
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 Pseudomonas putida