EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.32 | additional information | enzyme induction by 4-chlorobenzenesulfonic acid | Pseudomonas aeruginosa | |
1.13.11.1 | additional information | enzyme induction by 4-chlorobenzenesulfonic acid | Pseudomonas aeruginosa | |
3.1.1.45 | additional information | enzyme induction by 4-chlorobenzenesulfonic acid is required for enzyme expression and activity | Pseudomonas aeruginosa | |
5.5.1.1 | additional information | enzyme induction by 4-chlorobenzenesulfonic acid | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | Pseudomonas aeruginosa | - |
3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | Pseudomonas aeruginosa RW41 | - |
3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | additional information | Pseudomonas aeruginosa | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? | |
1.3.1.32 | additional information | Pseudomonas aeruginosa RW41 | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? | |
1.13.11.1 | catechol + O2 | Pseudomonas aeruginosa | - |
cis,cis-muconate | - |
? | |
1.13.11.1 | additional information | Pseudomonas aeruginosa | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? | |
3.1.1.45 | additional information | Pseudomonas aeruginosa | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | Pseudomonas aeruginosa | specific for the trans-isomer, no activity with the cis-isomer | maleylacetate | - |
? | |
5.5.1.1 | cis,cis-muconate | Pseudomonas aeruginosa | i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity | muconolactone | i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate | ? | |
5.5.1.1 | cis,cis-muconate | Pseudomonas aeruginosa RW41 | i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity | muconolactone | i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate | ? | |
5.5.1.1 | additional information | Pseudomonas aeruginosa | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? | |
5.5.1.1 | additional information | Pseudomonas aeruginosa RW41 | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.3.1.32 | Pseudomonas aeruginosa | O87612 | - |
- |
1.3.1.32 | Pseudomonas aeruginosa RW41 | O87612 | - |
- |
1.13.11.1 | Pseudomonas aeruginosa | - |
- |
- |
1.13.11.1 | Pseudomonas aeruginosa RW41 | - |
- |
- |
1.13.11.2 | no activity in Pseudomonas aeruginosa | - |
- |
- |
1.13.11.2 | no activity in Pseudomonas aeruginosa RW41 | - |
- |
- |
3.1.1.45 | Pseudomonas aeruginosa | - |
- |
- |
3.1.1.45 | Pseudomonas aeruginosa RW41 | - |
- |
- |
5.5.1.1 | Pseudomonas aeruginosa | - |
- |
- |
5.5.1.1 | Pseudomonas aeruginosa RW41 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.3.1.32 | native enzyme partially by anion exchange chromatography | Pseudomonas aeruginosa |
1.13.11.1 | native enzyme partially by anion exchange chromatography | Pseudomonas aeruginosa |
3.1.1.45 | native enzyme partially by anion exchange chromatography | Pseudomonas aeruginosa |
5.5.1.1 | native enzyme partially by anion exchange chromatography | Pseudomonas aeruginosa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.3.1.32 | additional information | the enzyme is only present when cells grow on 4-chlorobenzenesulfonic acid | Pseudomonas aeruginosa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.3.1.32 | 0.0034 | - |
substrate 2-maleylacetate, crude enzyme extract | Pseudomonas aeruginosa |
1.13.11.1 | 0.041 | - |
substrate 3-chlorocatechol, crude enzyme extract | Pseudomonas aeruginosa |
1.13.11.1 | 0.137 | - |
substrate 4-chlorocatechol, crude enzyme extract | Pseudomonas aeruginosa |
1.13.11.1 | 0.246 | - |
substrate catechol, crude enzyme extract | Pseudomonas aeruginosa |
3.1.1.45 | 0.02 | - |
substrate trans-dienelactone, crude enzyme extract of cells induced by 4-chlorobenzenesulfonic acid | Pseudomonas aeruginosa |
5.5.1.1 | 0.017 | - |
substrate 3-chloro-cis,cis-muconate, crude enzyme extrat | Pseudomonas aeruginosa |
5.5.1.1 | 0.035 | - |
substrate cis,cis-muconate, crude enzyme extract | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | - |
Pseudomonas aeruginosa | 3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | i.e. 3-hydroxymuconate | Pseudomonas aeruginosa | 3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | - |
Pseudomonas aeruginosa RW41 | 3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | 2-maleylacetate + NAD(P)H + H+ | i.e. 3-hydroxymuconate | Pseudomonas aeruginosa RW41 | 3-oxoadipate + NAD(P)+ | - |
r | |
1.3.1.32 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa | ? | - |
? | |
1.3.1.32 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa RW41 | ? | - |
? | |
1.13.11.1 | 3-chlorocatechol + O2 | - |
Pseudomonas aeruginosa | 2-chloro-cis,cis-muconate | - |
? | |
1.13.11.1 | 4-chlorocatechol + O2 | - |
Pseudomonas aeruginosa | 4-chlorocis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | - |
Pseudomonas aeruginosa | cis,cis-muconate | - |
? | |
1.13.11.1 | catechol + O2 | - |
Pseudomonas aeruginosa | cis,cis-muconate | i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate | ? | |
1.13.11.1 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa | ? | - |
? | |
1.13.11.1 | additional information | no activity with 4-sulfocatechol | Pseudomonas aeruginosa | ? | - |
? | |
3.1.1.45 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa | ? | - |
? | |
3.1.1.45 | additional information | no activity with protoanemonin | Pseudomonas aeruginosa | ? | - |
? | |
3.1.1.45 | trans-dienelactone + H2O | specific for the trans-isomer, no activity with the cis-isomer | Pseudomonas aeruginosa | maleylacetate | - |
? | |
5.5.1.1 | 3-chloro-cis,cis-muconate | - |
Pseudomonas aeruginosa | 4-chloro-muconolactone | unstable | ? | |
5.5.1.1 | 3-chloro-cis,cis-muconate | - |
Pseudomonas aeruginosa RW41 | 4-chloro-muconolactone | unstable | ? | |
5.5.1.1 | 3-chloro-cis,cis-muconate | in vitro activity | Pseudomonas aeruginosa | cis-dienelactone + protoanemonin + Cl- + H2O + CO2 | product ratio is 76:24, protoanemonin is antibiotically active | ? | |
5.5.1.1 | 3-chloro-cis,cis-muconate | in vitro activity | Pseudomonas aeruginosa RW41 | cis-dienelactone + protoanemonin + Cl- + H2O + CO2 | product ratio is 76:24, protoanemonin is antibiotically active | ? | |
5.5.1.1 | cis,cis-muconate | - |
Pseudomonas aeruginosa | muconolactone | - |
? | |
5.5.1.1 | cis,cis-muconate | i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity | Pseudomonas aeruginosa | muconolactone | i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate | ? | |
5.5.1.1 | cis,cis-muconate | - |
Pseudomonas aeruginosa RW41 | muconolactone | - |
? | |
5.5.1.1 | cis,cis-muconate | i.e. cis,cis-hexadienedioate or (2Z,4Z)-hexa-2,4-dienedioate, in vitro activity | Pseudomonas aeruginosa RW41 | muconolactone | i.e. (2,5-dihydro-5-oxofuran-2-yl)acetate | ? | |
5.5.1.1 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa | ? | - |
? | |
5.5.1.1 | additional information | the enzyme is involved in degradation of 4-chlorobenzenesulfonic acid, 4CBSA, the major polar by-product of the chemical synthesis of 1,1,1-trichloro-2,2-bis-(4-chlorophenyl) ethane, DDT, overview, 4-chlorobenzenesulfonic acid catabolic pathway, overview | Pseudomonas aeruginosa RW41 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.3.1.32 | maleylacetate reductase | - |
Pseudomonas aeruginosa |
1.13.11.1 | (chloro-)catechol 1,2-dioxygenase | - |
Pseudomonas aeruginosa |
1.13.11.1 | C12O | - |
Pseudomonas aeruginosa |
3.1.1.45 | dienelactone hydrolase | - |
Pseudomonas aeruginosa |
3.1.1.45 | rans-DLH | - |
Pseudomonas aeruginosa |
5.5.1.1 | (chloro)-muconate cycloisomerase | - |
Pseudomonas aeruginosa |
5.5.1.1 | MCI | - |
Pseudomonas aeruginosa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.3.1.32 | NAD(P)+ | - |
Pseudomonas aeruginosa | |
1.3.1.32 | NAD(P)H | - |
Pseudomonas aeruginosa |