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Literature summary extracted from

  • Rubio, M.B.; Cardoza, R.E.; Hermosa, R.; Gutierrez, S.; Monte, E.
    Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianum T34 (2008), Curr. Genet., 54, 301-312.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.74 16-hydroxyhexadecanoic acid is strongly induced in vitro by cutin monomer 16-hydroxyhexadecanoic acid Trichoderma harzianum
3.1.1.74 olive oil Thcut1 mRNA is strongly induced in vitro by olive oil Trichoderma harzianum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.74 expressed in Pichia pastoris Trichoderma harzianum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.74 D-glucose Thcut1 mRNA is repressed by glucose Trichoderma harzianum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.74 0.085
-
 p-nitrophenyl palmitate
-
 Trichoderma harzianum
3.1.1.74 0.33
-
 p-nitrophenyl acetate
-
 Trichoderma harzianum
3.1.1.74 0.57
-
 p-nitrophenyl butyrate
-
 Trichoderma harzianum
3.1.1.74 0.82
-
 p-nitrophenyl valerate
-
 Trichoderma harzianum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.74 extracellular
-
 Trichoderma harzianum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.74 29000
-
 SDS-PAGE Trichoderma harzianum

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.74 Trichoderma harzianum A8QPD8 T34
-
3.1.1.74 Trichoderma harzianum T34 A8QPD8 T34
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.74 0.004
-
 4-nitrophenyl palmitate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates Trichoderma harzianum
3.1.1.74 0.12
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h Trichoderma harzianum
3.1.1.74 0.25
-
 4 h cultures against 4-nitrophenyl palmitate Trichoderma harzianum
3.1.1.74 0.4
-
 4-nitrophenyl valerate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates Trichoderma harzianum
3.1.1.74 0.74
-
 4-nitrophenyl butyrate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates Trichoderma harzianum
3.1.1.74 0.85
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h Trichoderma harzianum
3.1.1.74 1.1
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h Trichoderma harzianum
3.1.1.74 1.2
-
 supernatant from 8 h culture on 0.2% cutin monomer 16-hydroxy-hexadecanoic acid shows slightly higher values against 4-nitrophenyl palmitate Trichoderma harzianum
3.1.1.74 1.3
-
 supernatant from 8 h culture on 0.05% cutin monomer 16-hydroxy-hexadecanoic acid shows slightly higher values against 4-nitrophenyl palmitate Trichoderma harzianum
3.1.1.74 1.8
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 1.8, 1.1, and 0.12 micromol/min/mg against 4-nitrophenyl palmitate, at 4, 8 and 24 h Trichoderma harzianum
3.1.1.74 2.84
-
 4-nitrophenyl acetate, esterase activity of the recombinant THCUT1 protein against different chromogenic substrates Trichoderma harzianum
3.1.1.74 4.2
-
 4 h cultures against 4-nitrophenyl butyrate (6.6 and 4.2 micromol/min/mg) Trichoderma harzianum
3.1.1.74 5.4
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h Trichoderma harzianum
3.1.1.74 6.6
-
 4 h cultures against 4-nitrophenyl butyrate (6.6 and 4.2 micromol/min/mg) Trichoderma harzianum
3.1.1.74 7.5
-
 supernatant from 8 h culture on 0.2% cutin monomer 16-hydroxy-hexadecanoic acid shows higher activity values against 4-nitrophenyl butyrate Trichoderma harzianum
3.1.1.74 9.8
-
 supernatant from 8 h culture on 0.05% cutin monomer 16-hydroxy-hexadecanoic acid shows higher activity values against 4-nitrophenyl butyrate Trichoderma harzianum
3.1.1.74 11.4
-
 in 1% olive oil, esterase activity values decreased after 4 h of incubation and are 11.4, 5.4, and 0.85 micromol/min/mg against 4-nitrophenyl butyrate, at 4, 8 and 24 h Trichoderma harzianum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.74 4-nitrophenyl acetate + H2O
-
 Trichoderma harzianum 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl acetate + H2O
-
 Trichoderma harzianum T34 4-nitrophenol + acetate
-
 ?
3.1.1.74 4-nitrophenyl butyrate + H2O
-
 Trichoderma harzianum 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl butyrate + H2O
-
 Trichoderma harzianum T34 4-nitrophenol + butyrate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O
-
 Trichoderma harzianum 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl palmitate + H2O
-
 Trichoderma harzianum T34 4-nitrophenol + palmitate
-
 ?
3.1.1.74 4-nitrophenyl valerate + H2O
-
 Trichoderma harzianum 4-nitrophenol + pentanoate
-
 ?
3.1.1.74 4-nitrophenyl valerate + H2O
-
 Trichoderma harzianum T34 4-nitrophenol + pentanoate
-
 ?
3.1.1.74 cutin + H2O
-
 Trichoderma harzianum cutin monomers
-
 ?
3.1.1.74 p-nitrophenyl valerate + H2O
-
 Trichoderma harzianum p-nitrophenol + pentanoate
-
 ?
3.1.1.74 p-nitrophenyl valerate + H2O
-
 Trichoderma harzianum T34 p-nitrophenol + pentanoate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.1.74 cutinase
-
 Trichoderma harzianum
3.1.1.74 Thcut1
-
 Trichoderma harzianum
3.1.1.74 THCUT1 protein
-
 Trichoderma harzianum