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Literature summary extracted from

  • Sandigursky, M.; Franklin, W.A.
    Thermostable uracil-DNA glycosylase from Thermotoga maritima, a member of a novel class of DNA repair enzymes (1999), Curr. Biol., 9, 531-534.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.2.27 gene udg, DNA and amino acid sequence determination, analysis and comparison, expression of His-tagged UDG in Escherichia coli strain BW310(DE3) Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Thermotoga maritima UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview uracil + double-stranded DNA with abasic site
-
?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview uracil + double-stranded DNA with abasic site
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.2.27 Thermotoga maritima
-
-
-
3.2.2.27 Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.2.27 recombinant His-tagged UDG from Escherichia coli strain BW310(DE3) by nickel affinity chromatography and gel filtration Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.2.27 uracil-mismatched double-stranded DNA + H2O UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview Thermotoga maritima uracil + double-stranded DNA with abasic site
-
?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O TMUDG removes uracil from double-stranded oligonucleotides containing either a U-G or a U-A base pair, e.g. from a 30mer ds oligonucleotide, DNA containing 3H-labeled uracil substrate preparation by nick translation of calf thymus DNA, overview Thermotoga maritima uracil + double-stranded DNA with abasic site
-
?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O UDG is a DNA repair enzyme removing uracil bases that are present in DNA as a result of either deamination of cytosine or misincorporation of dUMP instead of dTMP, and it is the primary activity in the DNA base excision repair pathway, overview Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 uracil + double-stranded DNA with abasic site
-
?
3.2.2.27 uracil-mismatched double-stranded DNA + H2O TMUDG removes uracil from double-stranded oligonucleotides containing either a U-G or a U-A base pair, e.g. from a 30mer ds oligonucleotide, DNA containing 3H-labeled uracil substrate preparation by nick translation of calf thymus DNA, overview Thermotoga maritima MSB8 / DSM 3109 / ATCC 43589 uracil + double-stranded DNA with abasic site
-
?

Synonyms

EC Number Synonyms Comment Organism
3.2.2.27 More Thermotoga maritima UDG is a member of one of three classes of uracil-DNA glycosylases Thermotoga maritima
3.2.2.27 TMUDG
-
Thermotoga maritima
3.2.2.27 UDG
-
Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.2.27 50
-
assay at Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.2.27 37 75
-
Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.2.27 7.8
-
assay at Thermotoga maritima