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Literature summary extracted from
- Physical immobilization of Rhizopus oryzae lipase onto cellulose substrate: activity and stability studies (2008), Colloids Surf. B Biointerfaces, 66, 168-177.
General Stability
| EC Number | General Stability | Organism |
|---|---|---|
| 3.1.1.3 | immobilized lipase carried on Cell-450 oxidized cellulose fibers shows an activity yield of 15% | Rhizopus arrhizus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.3 | Rhizopus arrhizus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.3 | partially purified by ammonium sulfate precipitation | Rhizopus arrhizus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.3 | oleic acid + n-butanol | - |
Rhizopus arrhizus | ? | - |
? |  |
| 3.1.1.3 | olive oil + H2O | - |
Rhizopus arrhizus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.3 | lipase | - |
Rhizopus arrhizus |
| 3.1.1.3 | triacylglycerol ester hydrolase | - |
Rhizopus arrhizus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 25 | 50 | the activity of the free lipase is maintained roughly constant until temperature 37°C, above which a continuous decrease in the activity occurs, the activity is preserved till 45°C for the enzyme immobilized on Cell-450 oxidized cellulose fibers | Rhizopus arrhizus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 8.3 | - |
around pH 8.3 | Rhizopus arrhizus |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 7.5 | 9.5 | - |
Rhizopus arrhizus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.3 | 6 | 8.3 | activity of the free lipase drops rapidly above pH 8.3, immobilized lipase is stable within the pH range 3-8, the free lipase is stable only at pH 6 | Rhizopus arrhizus |
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