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Literature summary extracted from

  • Dippe, M.; Mrestani-Klaus, C.; Schierhorn, A.; Ulbrich-Hofmann, R.
    Phospholipase D-catalyzed synthesis of new phospholipids with polar head groups (2008), Chem. Phys. Lipids, 152, 71-77.
    View publication on PubMed

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.4 Ca2+
-
Streptomyces sp.
3.1.4.4 Ca2+
-
Brassica oleracea

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.4 Brassica oleracea
-
-
-
3.1.4.4 Streptomyces sp.
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.4 0.006
-
transphosphatidylation reaction, substrate: 2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol, specific activity of hydrolysis reaction: 0.029 micromol/min/mg Brassica oleracea
3.1.4.4 2.28
-
transphosphatidylation reaction, substrate: tris(hydroxymethyl)-aminomethane, specific activity of hydrolysis reaction: 0.90 micromol/min/mg Brassica oleracea
3.1.4.4 4.08
-
transphosphatidylation reaction, substrate: triethanolamine, specific activity of hydrolysis reaction: 5.68 micromol/min/mg Brassica oleracea
3.1.4.4 6.18
-
transphosphatidylation reaction, substrate: diethanolamine, specific activity of hydrolysis reaction: 0.64 micromol/min/mg Brassica oleracea
3.1.4.4 7.53
-
transphosphatidylation reaction, substrate: serinol, specific activity of hydrolysis reaction: 0.83 micromol/min/mg Brassica oleracea
3.1.4.4 9.19
-
transphosphatidylation reaction, substrate: ethanolamine, specific activity of hydrolysis reaction: 0.65 micromol/min/mg Brassica oleracea
3.1.4.4 38.5
-
transphosphatidylation reaction, substrate: 2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol, specific activity of hydrolysis reaction: 22 micromol/min/mg Streptomyces sp.
3.1.4.4 124
-
transphosphatidylation reaction, substrate: tris(hydroxymethyl)-aminomethane, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
3.1.4.4 223.5
-
transphosphatidylation reaction, substrate: ethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
3.1.4.4 380.3
-
transphosphatidylation reaction, substrate: diethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
3.1.4.4 410.1
-
transphosphatidylation reaction, substrate: triethanolamine, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.
3.1.4.4 489.7
-
transphosphatidylation reaction, substrate: serinol, specific activity of hydrolysis reaction: value below 5 micromol/min/mg Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + 2-[bis(2-hydroxyethyl)amino]-2-(hydroxymethyl)-1,3-propanediol with BisTris as acceptor alcohol two regioisomeric forms of phosphatidyl-BisTris are obtained, product yield: 19.6% (regioisomer I), 3,1% (regioisomer II), purity of product: 98% (regioisomer I), 97% (regioisomer I) Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-2-[bis(2-hydroxyethyl)imino]-2-(hydroxymethyl)-1,3-propanediol
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine product yield: 44.1%, purity of product: 97.0% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-diethanolamine
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine product yield: 47.2%, purity of product: 81.5% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-diethanolamine
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + H2O
-
Streptomyces sp. choline + 1,2-dioleoyl-sn-glycero-3-phosphatidic acid
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + serinol product yield: 39.5%, purity of product: 99.8% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-serinol
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + serinol product yield: 45.8%, purity of product: 68% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-serinol
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine product yield: 22.6%, purity of product: 99.5% Brassica oleracea choline + 1,2-dioleoyl-sn-glycerophospho-triethanolamine
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine product yield: 33.7%, purity of product: 65.4% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-triethanolamine
-
?
3.1.4.4 1,2-dioleoyl-sn-glycerophosphocholine + tris(hydroxymethyl)-aminomethane product yield: 31.6%, purity of product: 95% Streptomyces sp. choline + 1,2-dioleoyl-sn-glycerophospho-tris(hydroxymethyl)-aminomethane
-
?
3.1.4.4 2 1,2-dioleoyl-sn-glycerophosphocholine + diethanolamine
-
Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-diethanolamine
-
?
3.1.4.4 2 1,2-dioleoyl-sn-glycerophosphocholine + serinol
-
Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-serinol
-
?
3.1.4.4 2 1,2-dioleoyl-sn-glycerophosphocholine + triethanolamine
-
Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-triethanolamine
-
?
3.1.4.4 2 1,2-dioleoyl-sn-glycerophosphocholine + tris(hydroxymethyl)-aminomethane
-
Streptomyces sp. 2 choline + bis(1,2-dioleoyl-sn-glycerophospho)-tris(hydroxymethyl)-aminomethane
-
?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.4 phospholipase D
-
Streptomyces sp.
3.1.4.4 phospholipase D
-
Brassica oleracea
3.1.4.4 PLDcab
-
Brassica oleracea
3.1.4.4 PLDStr
-
Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.4 30
-
assay at Streptomyces sp.
3.1.4.4 30
-
assay at Brassica oleracea

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.4 5.6
-
assay at Streptomyces sp.
3.1.4.4 5.6
-
assay at Brassica oleracea